Cargando…

Dried blood spot cards: A reliable sampling method to detect human antibodies against rabies virus

BACKGROUND: Although preventable by vaccination for more than a century, rabies virus still causes numerous fatalities every year. To determine antibody levels in humans, blood collected with a finger prick and applied on dried blood spot (DBS) cards is an alternative for venipuncture. The use of DB...

Descripción completa

Detalles Bibliográficos
Autores principales: Doornekamp, Laura, Embregts, Carmen W. E., Aron, Georgina I., Goeijenbier, Simone, van de Vijver, David A. M. C., van Gorp, Eric C. M., GeurtsvanKessel, Corine H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584180/
https://www.ncbi.nlm.nih.gov/pubmed/33048925
http://dx.doi.org/10.1371/journal.pntd.0008784
_version_ 1783599543242194944
author Doornekamp, Laura
Embregts, Carmen W. E.
Aron, Georgina I.
Goeijenbier, Simone
van de Vijver, David A. M. C.
van Gorp, Eric C. M.
GeurtsvanKessel, Corine H.
author_facet Doornekamp, Laura
Embregts, Carmen W. E.
Aron, Georgina I.
Goeijenbier, Simone
van de Vijver, David A. M. C.
van Gorp, Eric C. M.
GeurtsvanKessel, Corine H.
author_sort Doornekamp, Laura
collection PubMed
description BACKGROUND: Although preventable by vaccination for more than a century, rabies virus still causes numerous fatalities every year. To determine antibody levels in humans, blood collected with a finger prick and applied on dried blood spot (DBS) cards is an alternative for venipuncture. The use of DBS is specifically valuable in remote areas, as it is easy to perform, store and transport. Therefore, the technique is frequently used for epidemiological studies of tropical diseases. Up to present, determination of rabies virus antibody levels on human DBS has not been validated. METHODOLOGY/PRINCIPAL FINDINGS: We evaluated the use of human DBS for rabies serology and analyzed 99 pre- or post-vaccination serum and DBS samples with a fluorescent antibody virus neutralization test (FAVNt), which is the gold standard to detect protective antibody levels, and a Bio-Rad Platelia Rabies II ELISA. Sensitivity and specificity of DBS eluates tested with the FAVNt were 97% and 92%, respectively and 87% and 96% when tested with the Platelia-II ELISA. Antibody levels measured in serum with the FAVNt, correlated best with antibody levels measured in DBS with the FAVNt (R = 0.88). CONCLUSIONS/SIGNIFICANCE: This is the first study that applies DBS for reliable detection of human antibodies against rabies virus. Both the FAVNt and Platelia-II ELISA demonstrate an acceptable performance on DBS, providing opportunities for rabies serology in remote areas. This technique could drastically ease studies evaluating (novel) rabies vaccination strategies and monitoring persisting immunity in humans at risk, living in rabies endemic regions.
format Online
Article
Text
id pubmed-7584180
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-75841802020-10-27 Dried blood spot cards: A reliable sampling method to detect human antibodies against rabies virus Doornekamp, Laura Embregts, Carmen W. E. Aron, Georgina I. Goeijenbier, Simone van de Vijver, David A. M. C. van Gorp, Eric C. M. GeurtsvanKessel, Corine H. PLoS Negl Trop Dis Research Article BACKGROUND: Although preventable by vaccination for more than a century, rabies virus still causes numerous fatalities every year. To determine antibody levels in humans, blood collected with a finger prick and applied on dried blood spot (DBS) cards is an alternative for venipuncture. The use of DBS is specifically valuable in remote areas, as it is easy to perform, store and transport. Therefore, the technique is frequently used for epidemiological studies of tropical diseases. Up to present, determination of rabies virus antibody levels on human DBS has not been validated. METHODOLOGY/PRINCIPAL FINDINGS: We evaluated the use of human DBS for rabies serology and analyzed 99 pre- or post-vaccination serum and DBS samples with a fluorescent antibody virus neutralization test (FAVNt), which is the gold standard to detect protective antibody levels, and a Bio-Rad Platelia Rabies II ELISA. Sensitivity and specificity of DBS eluates tested with the FAVNt were 97% and 92%, respectively and 87% and 96% when tested with the Platelia-II ELISA. Antibody levels measured in serum with the FAVNt, correlated best with antibody levels measured in DBS with the FAVNt (R = 0.88). CONCLUSIONS/SIGNIFICANCE: This is the first study that applies DBS for reliable detection of human antibodies against rabies virus. Both the FAVNt and Platelia-II ELISA demonstrate an acceptable performance on DBS, providing opportunities for rabies serology in remote areas. This technique could drastically ease studies evaluating (novel) rabies vaccination strategies and monitoring persisting immunity in humans at risk, living in rabies endemic regions. Public Library of Science 2020-10-13 /pmc/articles/PMC7584180/ /pubmed/33048925 http://dx.doi.org/10.1371/journal.pntd.0008784 Text en © 2020 Doornekamp et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Doornekamp, Laura
Embregts, Carmen W. E.
Aron, Georgina I.
Goeijenbier, Simone
van de Vijver, David A. M. C.
van Gorp, Eric C. M.
GeurtsvanKessel, Corine H.
Dried blood spot cards: A reliable sampling method to detect human antibodies against rabies virus
title Dried blood spot cards: A reliable sampling method to detect human antibodies against rabies virus
title_full Dried blood spot cards: A reliable sampling method to detect human antibodies against rabies virus
title_fullStr Dried blood spot cards: A reliable sampling method to detect human antibodies against rabies virus
title_full_unstemmed Dried blood spot cards: A reliable sampling method to detect human antibodies against rabies virus
title_short Dried blood spot cards: A reliable sampling method to detect human antibodies against rabies virus
title_sort dried blood spot cards: a reliable sampling method to detect human antibodies against rabies virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584180/
https://www.ncbi.nlm.nih.gov/pubmed/33048925
http://dx.doi.org/10.1371/journal.pntd.0008784
work_keys_str_mv AT doornekamplaura driedbloodspotcardsareliablesamplingmethodtodetecthumanantibodiesagainstrabiesvirus
AT embregtscarmenwe driedbloodspotcardsareliablesamplingmethodtodetecthumanantibodiesagainstrabiesvirus
AT arongeorginai driedbloodspotcardsareliablesamplingmethodtodetecthumanantibodiesagainstrabiesvirus
AT goeijenbiersimone driedbloodspotcardsareliablesamplingmethodtodetecthumanantibodiesagainstrabiesvirus
AT vandevijverdavidamc driedbloodspotcardsareliablesamplingmethodtodetecthumanantibodiesagainstrabiesvirus
AT vangorpericcm driedbloodspotcardsareliablesamplingmethodtodetecthumanantibodiesagainstrabiesvirus
AT geurtsvankesselcorineh driedbloodspotcardsareliablesamplingmethodtodetecthumanantibodiesagainstrabiesvirus