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Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer

Culture medium composition is one of the most important parameters to analyze in biotechnological processes with industrial purposes. The aim of this study was to design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and b...

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Autores principales: Morales-Borrell, Dayana, González-Fernández, Nemecio, Mora-González, Néstor, Pérez-Heredia, Carlos, Campal-Espinosa, Ana, Bover-Fuentes, Eddy, Salazar-Gómez, Eladio, Morales-Espinosa, Yissel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584722/
https://www.ncbi.nlm.nih.gov/pubmed/33095435
http://dx.doi.org/10.1186/s13568-020-01127-y
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author Morales-Borrell, Dayana
González-Fernández, Nemecio
Mora-González, Néstor
Pérez-Heredia, Carlos
Campal-Espinosa, Ana
Bover-Fuentes, Eddy
Salazar-Gómez, Eladio
Morales-Espinosa, Yissel
author_facet Morales-Borrell, Dayana
González-Fernández, Nemecio
Mora-González, Néstor
Pérez-Heredia, Carlos
Campal-Espinosa, Ana
Bover-Fuentes, Eddy
Salazar-Gómez, Eladio
Morales-Espinosa, Yissel
author_sort Morales-Borrell, Dayana
collection PubMed
description Culture medium composition is one of the most important parameters to analyze in biotechnological processes with industrial purposes. The aim of this study was to design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer. The influence of several carbon and nitrogen sources and their molar ratios on P. indica H32 growth was investigated. The effect of different micronutrients such as mineral salts and vitamin on P. indica H32 growth was determined as well. A mixture design based on Design-Expert 10.0 Software was performed to optimize the culture medium concentration. Finally, in the designed medium, an attribute of the biological mechanism of action of the P. indica H32 against nematodes, was evaluated: the hydrogen sulfide production. It was found that tested carbon/nitrogen ratios were not a significant influence on P. indica H32 growth. Growth of P. indica H32 was favored with use of sucrose, yeast extract and phosphate buffer without the addition of any tested micronutrients. An optimal concentration of 10 g/L sucrose and 5 g/L yeast extract were obtained at a cost of 0.10 $/L. In this concentration, the specific growth rate (µ) and maximal optical density (Xmax) were equal to 0.439 h(− 1) and 8.00 respectively. It was evidenced that under the culture conditions used, P. indica H32 produced hydrogen sulfide. The designed medium led to a 1.08 $/L reduction of costs in comparison to LB medium. These results were critical to carry on with biotechnological development of P. indica H32 as a bioproduct.
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spelling pubmed-75847222020-10-26 Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer Morales-Borrell, Dayana González-Fernández, Nemecio Mora-González, Néstor Pérez-Heredia, Carlos Campal-Espinosa, Ana Bover-Fuentes, Eddy Salazar-Gómez, Eladio Morales-Espinosa, Yissel AMB Express Original Article Culture medium composition is one of the most important parameters to analyze in biotechnological processes with industrial purposes. The aim of this study was to design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer. The influence of several carbon and nitrogen sources and their molar ratios on P. indica H32 growth was investigated. The effect of different micronutrients such as mineral salts and vitamin on P. indica H32 growth was determined as well. A mixture design based on Design-Expert 10.0 Software was performed to optimize the culture medium concentration. Finally, in the designed medium, an attribute of the biological mechanism of action of the P. indica H32 against nematodes, was evaluated: the hydrogen sulfide production. It was found that tested carbon/nitrogen ratios were not a significant influence on P. indica H32 growth. Growth of P. indica H32 was favored with use of sucrose, yeast extract and phosphate buffer without the addition of any tested micronutrients. An optimal concentration of 10 g/L sucrose and 5 g/L yeast extract were obtained at a cost of 0.10 $/L. In this concentration, the specific growth rate (µ) and maximal optical density (Xmax) were equal to 0.439 h(− 1) and 8.00 respectively. It was evidenced that under the culture conditions used, P. indica H32 produced hydrogen sulfide. The designed medium led to a 1.08 $/L reduction of costs in comparison to LB medium. These results were critical to carry on with biotechnological development of P. indica H32 as a bioproduct. Springer Berlin Heidelberg 2020-10-23 /pmc/articles/PMC7584722/ /pubmed/33095435 http://dx.doi.org/10.1186/s13568-020-01127-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Morales-Borrell, Dayana
González-Fernández, Nemecio
Mora-González, Néstor
Pérez-Heredia, Carlos
Campal-Espinosa, Ana
Bover-Fuentes, Eddy
Salazar-Gómez, Eladio
Morales-Espinosa, Yissel
Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer
title Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer
title_full Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer
title_fullStr Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer
title_full_unstemmed Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer
title_short Design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer
title_sort design of a culture medium for optimal growth of the bacterium pseudoxanthomonas indica h32 allowing its production as biopesticide and biofertilizer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584722/
https://www.ncbi.nlm.nih.gov/pubmed/33095435
http://dx.doi.org/10.1186/s13568-020-01127-y
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