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Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H(2)O(2)-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency

BACKGROUND: Prolonged exposure of free radicals, or known as reactive oxygen species (ROS), in hepatic cells may cause oxidative stress. Without proper treatment, it can induce liver injury and fatal hepatic disease, including cirrhosis. Red betel (Piper crocatum Ruiz and Pav) is one of Indonesia’s...

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Autores principales: Lister, I. Nyoman Ehrich, Ginting, Chrismis Novalinda, Girsang, Ermi, Nataya, Enden Dea, Azizah, Alya Mardhotillah, Widowati, Wahyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584795/
https://www.ncbi.nlm.nih.gov/pubmed/33132711
http://dx.doi.org/10.1016/j.jsps.2020.08.007
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author Lister, I. Nyoman Ehrich
Ginting, Chrismis Novalinda
Girsang, Ermi
Nataya, Enden Dea
Azizah, Alya Mardhotillah
Widowati, Wahyu
author_facet Lister, I. Nyoman Ehrich
Ginting, Chrismis Novalinda
Girsang, Ermi
Nataya, Enden Dea
Azizah, Alya Mardhotillah
Widowati, Wahyu
author_sort Lister, I. Nyoman Ehrich
collection PubMed
description BACKGROUND: Prolonged exposure of free radicals, or known as reactive oxygen species (ROS), in hepatic cells may cause oxidative stress. Without proper treatment, it can induce liver injury and fatal hepatic disease, including cirrhosis. Red betel (Piper crocatum Ruiz and Pav) is one of Indonesia’s medicinal plants that has been known to exhibit antioxidant, anti-inflammatory activities. This study aims to determine hepatoprotective effect of red betel leaves extract (RBLE) towards liver injury. METHOD: Hydrogen peroxide-induced HepG2 cells were used as liver injury model·H(2)O(2)-induced HepG2 cells were treated with 25 µg/mL and 100 µg/mL RBLE. Several parameters were observed, including TNF-α level through ELISA; necrotic, apoptotic, dead, live cells; and ROS level through flow cytometry analysis; and GPX gene expression through qPCR. RESULT: The study showed that treatment with RBLE were able to decrease TNF-α level; necrotic and death cells percentage; as well as ROS level. On the other hand, it were able to increase apoptotic and live cells percentage; as well as GPX gene expression. Low concentration (25 µg/mL) of RBLE treatment exhibited stronger anti-inflammatory activity as it was resulted in the lower TNF-α level and were able to switched hepatic cell death pathway from necrosis to apoptosis as shown by the shifted of apoptotic cells and necrotic cells percentage. This lead to lower death cells and ultimately improve live cells percentage. Meanwhile high concentration of RBLE (100 µg/mL) exhibited stronger antioxidant properties as indicated by lower ROS level and higher GPX gene expression. CONCLUSION: Overall, this study was able to demonstrate hepatoprotective effect of RBLE towards liver injury model through its anti-inflammatory and antioxidant activities.
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spelling pubmed-75847952020-10-30 Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H(2)O(2)-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency Lister, I. Nyoman Ehrich Ginting, Chrismis Novalinda Girsang, Ermi Nataya, Enden Dea Azizah, Alya Mardhotillah Widowati, Wahyu Saudi Pharm J Original Article BACKGROUND: Prolonged exposure of free radicals, or known as reactive oxygen species (ROS), in hepatic cells may cause oxidative stress. Without proper treatment, it can induce liver injury and fatal hepatic disease, including cirrhosis. Red betel (Piper crocatum Ruiz and Pav) is one of Indonesia’s medicinal plants that has been known to exhibit antioxidant, anti-inflammatory activities. This study aims to determine hepatoprotective effect of red betel leaves extract (RBLE) towards liver injury. METHOD: Hydrogen peroxide-induced HepG2 cells were used as liver injury model·H(2)O(2)-induced HepG2 cells were treated with 25 µg/mL and 100 µg/mL RBLE. Several parameters were observed, including TNF-α level through ELISA; necrotic, apoptotic, dead, live cells; and ROS level through flow cytometry analysis; and GPX gene expression through qPCR. RESULT: The study showed that treatment with RBLE were able to decrease TNF-α level; necrotic and death cells percentage; as well as ROS level. On the other hand, it were able to increase apoptotic and live cells percentage; as well as GPX gene expression. Low concentration (25 µg/mL) of RBLE treatment exhibited stronger anti-inflammatory activity as it was resulted in the lower TNF-α level and were able to switched hepatic cell death pathway from necrosis to apoptosis as shown by the shifted of apoptotic cells and necrotic cells percentage. This lead to lower death cells and ultimately improve live cells percentage. Meanwhile high concentration of RBLE (100 µg/mL) exhibited stronger antioxidant properties as indicated by lower ROS level and higher GPX gene expression. CONCLUSION: Overall, this study was able to demonstrate hepatoprotective effect of RBLE towards liver injury model through its anti-inflammatory and antioxidant activities. Elsevier 2020-10 2020-08-18 /pmc/articles/PMC7584795/ /pubmed/33132711 http://dx.doi.org/10.1016/j.jsps.2020.08.007 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Lister, I. Nyoman Ehrich
Ginting, Chrismis Novalinda
Girsang, Ermi
Nataya, Enden Dea
Azizah, Alya Mardhotillah
Widowati, Wahyu
Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H(2)O(2)-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency
title Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H(2)O(2)-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency
title_full Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H(2)O(2)-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency
title_fullStr Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H(2)O(2)-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency
title_full_unstemmed Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H(2)O(2)-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency
title_short Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav) leaves extract towards H(2)O(2)-induced HepG2 cells via anti-inflammatory, antinecrotic, antioxidant potency
title_sort hepatoprotective properties of red betel (piper crocatum ruiz and pav) leaves extract towards h(2)o(2)-induced hepg2 cells via anti-inflammatory, antinecrotic, antioxidant potency
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584795/
https://www.ncbi.nlm.nih.gov/pubmed/33132711
http://dx.doi.org/10.1016/j.jsps.2020.08.007
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