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Evaluation of antibacterial properties of Matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria

BACKGROUND: Chronic periodontitis has an interplay between different species of bacteria found in dental biofilms act a crucial role in pathogenesis and disease progression. The existing antibacterial therapy is inadequate, associated with many side effects as well as evolving multidrug resistance....

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Autores principales: Ahmad, Irfan, Wahab, Shadma, Nisar, Nazima, Dera, Ayed A., Alshahrani, Mohammad Y, Abullias, Shahabe Saquib, Irfan, Safia, Alam, Mohammad Mahtab, Srivastava, Saurabh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584800/
https://www.ncbi.nlm.nih.gov/pubmed/33132714
http://dx.doi.org/10.1016/j.jsps.2020.08.010
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author Ahmad, Irfan
Wahab, Shadma
Nisar, Nazima
Dera, Ayed A.
Alshahrani, Mohammad Y
Abullias, Shahabe Saquib
Irfan, Safia
Alam, Mohammad Mahtab
Srivastava, Saurabh
author_facet Ahmad, Irfan
Wahab, Shadma
Nisar, Nazima
Dera, Ayed A.
Alshahrani, Mohammad Y
Abullias, Shahabe Saquib
Irfan, Safia
Alam, Mohammad Mahtab
Srivastava, Saurabh
author_sort Ahmad, Irfan
collection PubMed
description BACKGROUND: Chronic periodontitis has an interplay between different species of bacteria found in dental biofilms act a crucial role in pathogenesis and disease progression. The existing antibacterial therapy is inadequate, associated with many side effects as well as evolving multidrug resistance. Hence, novel drugs development with minimum or no toxicity is an immediate priority. METHODS: Antibacterial efficacy of ethanolic extract of Matricaria aurea was tested against clinical isolates, ie. Treponema denticol, Tannerella forsythia, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis from the patients with chronic periodontitis. Zone of inhibition, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were investigated by well diffusion method and micro broth dilution assay using alamar blue. Anti-virulence properties of the extract, which include adherence property and the biofilm formation, were investigated by adherence as well as biofilm formation assay. RESULTS: Matricaria aurea extract showed potent inhibitory effect against pathogenic periodontal bacteria with the significant inhibitory zone (13–23 mm), MIC (0.39–1.56 mg/ml) as well as MBC (1.56–6.25 mg/ml). The M. aurea extract was able to inhibit bacterial adhesion ranged from 30 to 45%, 35 to 63% and 55 to 80% of MIC at MIC × 0.5, MIC × 1 and MIC × 2 respectively. Significant inhibition was found in biofilm formation to all the tested periodontal bacterial strains after the treatment with various concentrations of M. aurea extract for 24 and 48hrs. CONCLUSION: These results reveal for the first time that the Matricaria aurea extract might be the source of various compounds to be applied for chronic periodontitis therapy, which might draw these valuable compounds to the subsequent phase of development of the drug.
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spelling pubmed-75848002020-10-30 Evaluation of antibacterial properties of Matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria Ahmad, Irfan Wahab, Shadma Nisar, Nazima Dera, Ayed A. Alshahrani, Mohammad Y Abullias, Shahabe Saquib Irfan, Safia Alam, Mohammad Mahtab Srivastava, Saurabh Saudi Pharm J Original Article BACKGROUND: Chronic periodontitis has an interplay between different species of bacteria found in dental biofilms act a crucial role in pathogenesis and disease progression. The existing antibacterial therapy is inadequate, associated with many side effects as well as evolving multidrug resistance. Hence, novel drugs development with minimum or no toxicity is an immediate priority. METHODS: Antibacterial efficacy of ethanolic extract of Matricaria aurea was tested against clinical isolates, ie. Treponema denticol, Tannerella forsythia, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis from the patients with chronic periodontitis. Zone of inhibition, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were investigated by well diffusion method and micro broth dilution assay using alamar blue. Anti-virulence properties of the extract, which include adherence property and the biofilm formation, were investigated by adherence as well as biofilm formation assay. RESULTS: Matricaria aurea extract showed potent inhibitory effect against pathogenic periodontal bacteria with the significant inhibitory zone (13–23 mm), MIC (0.39–1.56 mg/ml) as well as MBC (1.56–6.25 mg/ml). The M. aurea extract was able to inhibit bacterial adhesion ranged from 30 to 45%, 35 to 63% and 55 to 80% of MIC at MIC × 0.5, MIC × 1 and MIC × 2 respectively. Significant inhibition was found in biofilm formation to all the tested periodontal bacterial strains after the treatment with various concentrations of M. aurea extract for 24 and 48hrs. CONCLUSION: These results reveal for the first time that the Matricaria aurea extract might be the source of various compounds to be applied for chronic periodontitis therapy, which might draw these valuable compounds to the subsequent phase of development of the drug. Elsevier 2020-10 2020-08-17 /pmc/articles/PMC7584800/ /pubmed/33132714 http://dx.doi.org/10.1016/j.jsps.2020.08.010 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Ahmad, Irfan
Wahab, Shadma
Nisar, Nazima
Dera, Ayed A.
Alshahrani, Mohammad Y
Abullias, Shahabe Saquib
Irfan, Safia
Alam, Mohammad Mahtab
Srivastava, Saurabh
Evaluation of antibacterial properties of Matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria
title Evaluation of antibacterial properties of Matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria
title_full Evaluation of antibacterial properties of Matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria
title_fullStr Evaluation of antibacterial properties of Matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria
title_full_unstemmed Evaluation of antibacterial properties of Matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria
title_short Evaluation of antibacterial properties of Matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria
title_sort evaluation of antibacterial properties of matricaria aurea on clinical isolates of periodontitis patients with special reference to red complex bacteria
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584800/
https://www.ncbi.nlm.nih.gov/pubmed/33132714
http://dx.doi.org/10.1016/j.jsps.2020.08.010
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