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Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling
MicroProteins are small, often single-domain proteins that are sequence-related to larger, often multidomain proteins. Here, we used a combination of comparative genomics and heterologous synthetic misexpression to isolate functional cereal microProtein regulators. Our approach identified LITTLE NIN...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584889/ https://www.ncbi.nlm.nih.gov/pubmed/33033229 http://dx.doi.org/10.1073/pnas.2005198117 |
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author | Hong, Shin-Young Sun, Bin Straub, Daniel Blaakmeer, Anko Mineri, Lorenzo Koch, Jonas Brinch-Pedersen, Henrik Holme, Inger B. Burow, Meike Lyngs Jørgensen, Hans Jørgen Albà, M. Mar Wenkel, Stephan |
author_facet | Hong, Shin-Young Sun, Bin Straub, Daniel Blaakmeer, Anko Mineri, Lorenzo Koch, Jonas Brinch-Pedersen, Henrik Holme, Inger B. Burow, Meike Lyngs Jørgensen, Hans Jørgen Albà, M. Mar Wenkel, Stephan |
author_sort | Hong, Shin-Young |
collection | PubMed |
description | MicroProteins are small, often single-domain proteins that are sequence-related to larger, often multidomain proteins. Here, we used a combination of comparative genomics and heterologous synthetic misexpression to isolate functional cereal microProtein regulators. Our approach identified LITTLE NINJA (LNJ), a microProtein that acts as a modulator of jasmonic acid (JA) signaling. Ectopic expression of LNJ in Arabidopsis resulted in stunted plants that resembled the decuple JAZ (jazD) mutant. In fact, comparing the transcriptomes of transgenic LNJ overexpressor plants and jazD revealed a large overlap of deregulated genes, suggesting that ectopic LNJ expression altered JA signaling. Transgenic Brachypodium plants with elevated LNJ expression levels showed deregulation of JA signaling as well and displayed reduced growth and enhanced production of side shoots (tiller). This tillering effect was transferable between grass species, and overexpression of LNJ in barley and rice caused similar traits. We used a clustered regularly interspaced short palindromic repeats (CRISPR) approach and created a LNJ-like protein in Arabidopsis by deleting parts of the coding sentence of the AFP2 gene that encodes a NINJA-domain protein. These afp2-crispr mutants were also stunted in size and resembled jazD. Thus, similar genome-engineering approaches can be exploited as a future tool to create LNJ proteins and produce cereals with altered architectures. |
format | Online Article Text |
id | pubmed-7584889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-75848892020-10-30 Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling Hong, Shin-Young Sun, Bin Straub, Daniel Blaakmeer, Anko Mineri, Lorenzo Koch, Jonas Brinch-Pedersen, Henrik Holme, Inger B. Burow, Meike Lyngs Jørgensen, Hans Jørgen Albà, M. Mar Wenkel, Stephan Proc Natl Acad Sci U S A Biological Sciences MicroProteins are small, often single-domain proteins that are sequence-related to larger, often multidomain proteins. Here, we used a combination of comparative genomics and heterologous synthetic misexpression to isolate functional cereal microProtein regulators. Our approach identified LITTLE NINJA (LNJ), a microProtein that acts as a modulator of jasmonic acid (JA) signaling. Ectopic expression of LNJ in Arabidopsis resulted in stunted plants that resembled the decuple JAZ (jazD) mutant. In fact, comparing the transcriptomes of transgenic LNJ overexpressor plants and jazD revealed a large overlap of deregulated genes, suggesting that ectopic LNJ expression altered JA signaling. Transgenic Brachypodium plants with elevated LNJ expression levels showed deregulation of JA signaling as well and displayed reduced growth and enhanced production of side shoots (tiller). This tillering effect was transferable between grass species, and overexpression of LNJ in barley and rice caused similar traits. We used a clustered regularly interspaced short palindromic repeats (CRISPR) approach and created a LNJ-like protein in Arabidopsis by deleting parts of the coding sentence of the AFP2 gene that encodes a NINJA-domain protein. These afp2-crispr mutants were also stunted in size and resembled jazD. Thus, similar genome-engineering approaches can be exploited as a future tool to create LNJ proteins and produce cereals with altered architectures. National Academy of Sciences 2020-10-20 2020-10-08 /pmc/articles/PMC7584889/ /pubmed/33033229 http://dx.doi.org/10.1073/pnas.2005198117 Text en Copyright © 2020 the Author(s). Published by PNAS. http://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Biological Sciences Hong, Shin-Young Sun, Bin Straub, Daniel Blaakmeer, Anko Mineri, Lorenzo Koch, Jonas Brinch-Pedersen, Henrik Holme, Inger B. Burow, Meike Lyngs Jørgensen, Hans Jørgen Albà, M. Mar Wenkel, Stephan Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling |
title | Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling |
title_full | Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling |
title_fullStr | Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling |
title_full_unstemmed | Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling |
title_short | Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling |
title_sort | heterologous microprotein expression identifies little ninja, a dominant regulator of jasmonic acid signaling |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584889/ https://www.ncbi.nlm.nih.gov/pubmed/33033229 http://dx.doi.org/10.1073/pnas.2005198117 |
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