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Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines
Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptio...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584957/ https://www.ncbi.nlm.nih.gov/pubmed/33123207 http://dx.doi.org/10.1155/2020/4979850 |
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author | Kang, Qianli Wang, Yanyan Cui, Qinghua Gong, Lili Yang, Yong Jiang, Haiqiang Rong, Lijun Rong, Rong Du, Ruikun |
author_facet | Kang, Qianli Wang, Yanyan Cui, Qinghua Gong, Lili Yang, Yong Jiang, Haiqiang Rong, Lijun Rong, Rong Du, Ruikun |
author_sort | Kang, Qianli |
collection | PubMed |
description | Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptions for antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, n-butanol, and water. 500 TCM-simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess antiviral activities against influenza virus. The IC50's of the hit fractions range from 1.08 to 6.45 μg/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny influenza virus significantly at 20 μg/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti-influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating antiviral screening in quick response to emerging and re-emerging viruses such as Ebola virus and current SARS-CoV-2 pandemic. |
format | Online Article Text |
id | pubmed-7584957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-75849572020-10-28 Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines Kang, Qianli Wang, Yanyan Cui, Qinghua Gong, Lili Yang, Yong Jiang, Haiqiang Rong, Lijun Rong, Rong Du, Ruikun Evid Based Complement Alternat Med Research Article Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptions for antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, n-butanol, and water. 500 TCM-simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess antiviral activities against influenza virus. The IC50's of the hit fractions range from 1.08 to 6.45 μg/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny influenza virus significantly at 20 μg/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti-influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating antiviral screening in quick response to emerging and re-emerging viruses such as Ebola virus and current SARS-CoV-2 pandemic. Hindawi 2020-10-14 /pmc/articles/PMC7584957/ /pubmed/33123207 http://dx.doi.org/10.1155/2020/4979850 Text en Copyright © 2020 Qianli Kang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kang, Qianli Wang, Yanyan Cui, Qinghua Gong, Lili Yang, Yong Jiang, Haiqiang Rong, Lijun Rong, Rong Du, Ruikun Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines |
title | Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines |
title_full | Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines |
title_fullStr | Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines |
title_full_unstemmed | Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines |
title_short | Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines |
title_sort | screening for anti-influenza actives of prefractionated traditional chinese medicines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584957/ https://www.ncbi.nlm.nih.gov/pubmed/33123207 http://dx.doi.org/10.1155/2020/4979850 |
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