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Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines

Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptio...

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Autores principales: Kang, Qianli, Wang, Yanyan, Cui, Qinghua, Gong, Lili, Yang, Yong, Jiang, Haiqiang, Rong, Lijun, Rong, Rong, Du, Ruikun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584957/
https://www.ncbi.nlm.nih.gov/pubmed/33123207
http://dx.doi.org/10.1155/2020/4979850
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author Kang, Qianli
Wang, Yanyan
Cui, Qinghua
Gong, Lili
Yang, Yong
Jiang, Haiqiang
Rong, Lijun
Rong, Rong
Du, Ruikun
author_facet Kang, Qianli
Wang, Yanyan
Cui, Qinghua
Gong, Lili
Yang, Yong
Jiang, Haiqiang
Rong, Lijun
Rong, Rong
Du, Ruikun
author_sort Kang, Qianli
collection PubMed
description Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptions for antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, n-butanol, and water. 500 TCM-simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess antiviral activities against influenza virus. The IC50's of the hit fractions range from 1.08 to 6.45 μg/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny influenza virus significantly at 20 μg/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti-influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating antiviral screening in quick response to emerging and re-emerging viruses such as Ebola virus and current SARS-CoV-2 pandemic.
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spelling pubmed-75849572020-10-28 Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines Kang, Qianli Wang, Yanyan Cui, Qinghua Gong, Lili Yang, Yong Jiang, Haiqiang Rong, Lijun Rong, Rong Du, Ruikun Evid Based Complement Alternat Med Research Article Traditional Chinese medicines (TCMs) have proven to possess advantages in counteracting virus infections according to clinical practices. It's therefore of great value to discover novel antivirals from TCMs. In this paper, One hundred medicinal plants which have been included in TCM prescriptions for antiviral treatment were selected and prefractionated into 5 fractions each by sequentially using cyclohexane, dichloromethane, ethyl acetate, n-butanol, and water. 500 TCM-simplified extracts were then subjected to a phenotypic screening using a recombinant IAV expressing Gaussia luciferase. Ten TCM fractions were identified to possess antiviral activities against influenza virus. The IC50's of the hit fractions range from 1.08 to 6.45 μg/mL, while the SIs, from 7.52 to 98.40. Furthermore, all the ten hit fractions inhibited the propagation of progeny influenza virus significantly at 20 μg/mL. The hit TCM fractions deserve further isolation for responsible constituents leading towards anti-influenza drugs. Moreover, a library consisting of 500 simplified TCM extracts was established, facilitating antiviral screening in quick response to emerging and re-emerging viruses such as Ebola virus and current SARS-CoV-2 pandemic. Hindawi 2020-10-14 /pmc/articles/PMC7584957/ /pubmed/33123207 http://dx.doi.org/10.1155/2020/4979850 Text en Copyright © 2020 Qianli Kang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kang, Qianli
Wang, Yanyan
Cui, Qinghua
Gong, Lili
Yang, Yong
Jiang, Haiqiang
Rong, Lijun
Rong, Rong
Du, Ruikun
Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines
title Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines
title_full Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines
title_fullStr Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines
title_full_unstemmed Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines
title_short Screening for Anti-Influenza Actives of Prefractionated Traditional Chinese Medicines
title_sort screening for anti-influenza actives of prefractionated traditional chinese medicines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584957/
https://www.ncbi.nlm.nih.gov/pubmed/33123207
http://dx.doi.org/10.1155/2020/4979850
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