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Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase
PURPOSE: Corneal injury that occurs after burning with alkali initiates wound-healing processes, including inflammation, neovascularization, and fibrosis. Excessive reactions to injury can reduce corneal transparency and thereby compromise vision. The NADPH oxidase (Nox) enzyme complex is known to b...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585390/ https://www.ncbi.nlm.nih.gov/pubmed/33079994 http://dx.doi.org/10.1167/iovs.61.12.20 |
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author | Hakami, Nora Y. Dusting, Gregory J. Chan, Elsa C. Shah, Manisha H. Peshavariya, Hitesh M. |
author_facet | Hakami, Nora Y. Dusting, Gregory J. Chan, Elsa C. Shah, Manisha H. Peshavariya, Hitesh M. |
author_sort | Hakami, Nora Y. |
collection | PubMed |
description | PURPOSE: Corneal injury that occurs after burning with alkali initiates wound-healing processes, including inflammation, neovascularization, and fibrosis. Excessive reactions to injury can reduce corneal transparency and thereby compromise vision. The NADPH oxidase (Nox) enzyme complex is known to be involved in cell signaling for wound-healing angiogenesis, but its role in corneal neovascularization has been little studied. METHODS: The center corneas of wild-type and Nox4 knockout (KO) mice were injured with 3 µL 1 M NaOH, while the contralateral corneas remained untouched. On day 7, mRNA expression levels of NADPH oxidase isoforms, the proangiogenic factors VEGF-A and TGFβ1, and proinflammatory genes ICAM-1 and VCAM-1 were determined. Corneal neovascularization and fibrosis were visualized using PECAM-1 antibody and picrosirius red staining, respectively, on the same day. RESULTS: Expressions of both Nox2 and Nox4 gene isoforms as well as the above genes were markedly increased in the injured corneas at 7 days. Injured corneas showed neovascularization and fibrosis as well as an increase in clinical opacity score. All responses stimulated by alkali burn were abrogated in Nox4 KO mice. CONCLUSIONS: Nox4 could be a new target to treat pathologic corneal wound-healing responses and such targeting might prevent blindness caused by burn injuries. |
format | Online Article Text |
id | pubmed-7585390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-75853902020-11-03 Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase Hakami, Nora Y. Dusting, Gregory J. Chan, Elsa C. Shah, Manisha H. Peshavariya, Hitesh M. Invest Ophthalmol Vis Sci Cornea PURPOSE: Corneal injury that occurs after burning with alkali initiates wound-healing processes, including inflammation, neovascularization, and fibrosis. Excessive reactions to injury can reduce corneal transparency and thereby compromise vision. The NADPH oxidase (Nox) enzyme complex is known to be involved in cell signaling for wound-healing angiogenesis, but its role in corneal neovascularization has been little studied. METHODS: The center corneas of wild-type and Nox4 knockout (KO) mice were injured with 3 µL 1 M NaOH, while the contralateral corneas remained untouched. On day 7, mRNA expression levels of NADPH oxidase isoforms, the proangiogenic factors VEGF-A and TGFβ1, and proinflammatory genes ICAM-1 and VCAM-1 were determined. Corneal neovascularization and fibrosis were visualized using PECAM-1 antibody and picrosirius red staining, respectively, on the same day. RESULTS: Expressions of both Nox2 and Nox4 gene isoforms as well as the above genes were markedly increased in the injured corneas at 7 days. Injured corneas showed neovascularization and fibrosis as well as an increase in clinical opacity score. All responses stimulated by alkali burn were abrogated in Nox4 KO mice. CONCLUSIONS: Nox4 could be a new target to treat pathologic corneal wound-healing responses and such targeting might prevent blindness caused by burn injuries. The Association for Research in Vision and Ophthalmology 2020-10-20 /pmc/articles/PMC7585390/ /pubmed/33079994 http://dx.doi.org/10.1167/iovs.61.12.20 Text en Copyright 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Cornea Hakami, Nora Y. Dusting, Gregory J. Chan, Elsa C. Shah, Manisha H. Peshavariya, Hitesh M. Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase |
title | Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase |
title_full | Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase |
title_fullStr | Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase |
title_full_unstemmed | Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase |
title_short | Wound Healing After Alkali Burn Injury of the Cornea Involves Nox4-Type NADPH Oxidase |
title_sort | wound healing after alkali burn injury of the cornea involves nox4-type nadph oxidase |
topic | Cornea |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585390/ https://www.ncbi.nlm.nih.gov/pubmed/33079994 http://dx.doi.org/10.1167/iovs.61.12.20 |
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