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Stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus

OBJECTIVE(S): Chikungunya virus (ChikV) infection is characterized by persistent infection in joints and lymphoid organs. The ChikV Capsid protein plays an important role in regulating virus replication. In this study, we hypothesized that capsid protein may stimulate dendritic cell (DC) activation...

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Autores principales: Nghia, Vu Xuan, Giang, Nguyen Van, Canh, Nguyen Xuan, Ha, Nguyen Hai, Duong, Nguyen Thuy, Hoang, Nguyen Huy, Xuan, Nguyen Thi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585544/
https://www.ncbi.nlm.nih.gov/pubmed/33149858
http://dx.doi.org/10.22038/IJBMS.2020.40386.9558
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author Nghia, Vu Xuan
Giang, Nguyen Van
Canh, Nguyen Xuan
Ha, Nguyen Hai
Duong, Nguyen Thuy
Hoang, Nguyen Huy
Xuan, Nguyen Thi
author_facet Nghia, Vu Xuan
Giang, Nguyen Van
Canh, Nguyen Xuan
Ha, Nguyen Hai
Duong, Nguyen Thuy
Hoang, Nguyen Huy
Xuan, Nguyen Thi
author_sort Nghia, Vu Xuan
collection PubMed
description OBJECTIVE(S): Chikungunya virus (ChikV) infection is characterized by persistent infection in joints and lymphoid organs. The ChikV Capsid protein plays an important role in regulating virus replication. In this study, we hypothesized that capsid protein may stimulate dendritic cell (DC) activation and maturation and trigger an inflammatory response in mice. MATERIALS AND METHODS: Mice were intraperitoneally injected with capsid protein and examined for changes in immunophenotype in lymph nodes (LNs). Next, DCs were treated with capsid protein or LPS and then expression of maturation markers, cytokine production, and ability to stimulate CD4(+) T cells in allo-MLR were analyzed. RESULTS: Injection of mice with capsid protein led to recruitment of myeloid cells and increased activation of T lymphocytes in LNs. Importantly, treatment of DCs with capsid protein prolonged the activation of IKB-α and up-regulated the number of CD11c(+)CD86(+)DCs and release of TNF-α and IL-12p70 as well as reducing DC apoptosis, all effects were abolished in the presence of Bay 11-7082. In addition, IL-2 production was higher by CD4(+) T cells stimulated with capsid-treated as compared with LPS-induced DCs. CONCLUSION: The observations revealed that capsid protein participates in the regulation of NF-κB signaling and maturation of DCs.
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spelling pubmed-75855442020-11-03 Stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus Nghia, Vu Xuan Giang, Nguyen Van Canh, Nguyen Xuan Ha, Nguyen Hai Duong, Nguyen Thuy Hoang, Nguyen Huy Xuan, Nguyen Thi Iran J Basic Med Sci Original Article OBJECTIVE(S): Chikungunya virus (ChikV) infection is characterized by persistent infection in joints and lymphoid organs. The ChikV Capsid protein plays an important role in regulating virus replication. In this study, we hypothesized that capsid protein may stimulate dendritic cell (DC) activation and maturation and trigger an inflammatory response in mice. MATERIALS AND METHODS: Mice were intraperitoneally injected with capsid protein and examined for changes in immunophenotype in lymph nodes (LNs). Next, DCs were treated with capsid protein or LPS and then expression of maturation markers, cytokine production, and ability to stimulate CD4(+) T cells in allo-MLR were analyzed. RESULTS: Injection of mice with capsid protein led to recruitment of myeloid cells and increased activation of T lymphocytes in LNs. Importantly, treatment of DCs with capsid protein prolonged the activation of IKB-α and up-regulated the number of CD11c(+)CD86(+)DCs and release of TNF-α and IL-12p70 as well as reducing DC apoptosis, all effects were abolished in the presence of Bay 11-7082. In addition, IL-2 production was higher by CD4(+) T cells stimulated with capsid-treated as compared with LPS-induced DCs. CONCLUSION: The observations revealed that capsid protein participates in the regulation of NF-κB signaling and maturation of DCs. Mashhad University of Medical Sciences 2020-10 /pmc/articles/PMC7585544/ /pubmed/33149858 http://dx.doi.org/10.22038/IJBMS.2020.40386.9558 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Nghia, Vu Xuan
Giang, Nguyen Van
Canh, Nguyen Xuan
Ha, Nguyen Hai
Duong, Nguyen Thuy
Hoang, Nguyen Huy
Xuan, Nguyen Thi
Stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus
title Stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus
title_full Stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus
title_fullStr Stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus
title_full_unstemmed Stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus
title_short Stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus
title_sort stimulation of dendritic cell functional maturation by capsid protein from chikungunya virus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585544/
https://www.ncbi.nlm.nih.gov/pubmed/33149858
http://dx.doi.org/10.22038/IJBMS.2020.40386.9558
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