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Metabolic defects in splenic B cell compartments from patients with liver cirrhosis

Liver cirrhosis is associated with defective vaccine responses and increased infections. Dysregulated B cell compartments in cirrhotic patients have been noticed but not well characterized, especially in the spleen. Here, we comprehensively investigated B cell perturbations from the spleens and peri...

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Autores principales: Huang, Man, Liu, Xiaoju, Ye, Haocheng, Zhao, Xin, Zhao, Juanjuan, Liu, Yang, He, Xiaomeng, Qu, Mengmeng, Pan, Jing, Hou, Baidong, Cheng, Yongqian, Liu, Zhenwen, Li, Zhiwei, Liu, Lei, Sun, Jian, Zhang, Shuye, Zhang, Zheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585577/
https://www.ncbi.nlm.nih.gov/pubmed/33099582
http://dx.doi.org/10.1038/s41419-020-03060-1
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author Huang, Man
Liu, Xiaoju
Ye, Haocheng
Zhao, Xin
Zhao, Juanjuan
Liu, Yang
He, Xiaomeng
Qu, Mengmeng
Pan, Jing
Hou, Baidong
Cheng, Yongqian
Liu, Zhenwen
Li, Zhiwei
Liu, Lei
Sun, Jian
Zhang, Shuye
Zhang, Zheng
author_facet Huang, Man
Liu, Xiaoju
Ye, Haocheng
Zhao, Xin
Zhao, Juanjuan
Liu, Yang
He, Xiaomeng
Qu, Mengmeng
Pan, Jing
Hou, Baidong
Cheng, Yongqian
Liu, Zhenwen
Li, Zhiwei
Liu, Lei
Sun, Jian
Zhang, Shuye
Zhang, Zheng
author_sort Huang, Man
collection PubMed
description Liver cirrhosis is associated with defective vaccine responses and increased infections. Dysregulated B cell compartments in cirrhotic patients have been noticed but not well characterized, especially in the spleen. Here, we comprehensively investigated B cell perturbations from the spleens and peripheral blood of cirrhotic patients. We found that liver cirrhosis significantly depleted both switched and nonswitched splenic memory B cells, which was further confirmed histologically. Bulk RNA-seq revealed significant metabolic defects as the potential mechanism for the impaired splenic B cell functions. Functionally, the splenic memory B cells from cirrhotic patients showed strong metabolic defects and reduced proliferation compared with those from healthy controls. Thus, liver cirrhosis extensively disturbs the splenic and peripheral B cell compartments, which may contribute to defective humoral immunity during liver cirrhosis.
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spelling pubmed-75855772020-10-26 Metabolic defects in splenic B cell compartments from patients with liver cirrhosis Huang, Man Liu, Xiaoju Ye, Haocheng Zhao, Xin Zhao, Juanjuan Liu, Yang He, Xiaomeng Qu, Mengmeng Pan, Jing Hou, Baidong Cheng, Yongqian Liu, Zhenwen Li, Zhiwei Liu, Lei Sun, Jian Zhang, Shuye Zhang, Zheng Cell Death Dis Article Liver cirrhosis is associated with defective vaccine responses and increased infections. Dysregulated B cell compartments in cirrhotic patients have been noticed but not well characterized, especially in the spleen. Here, we comprehensively investigated B cell perturbations from the spleens and peripheral blood of cirrhotic patients. We found that liver cirrhosis significantly depleted both switched and nonswitched splenic memory B cells, which was further confirmed histologically. Bulk RNA-seq revealed significant metabolic defects as the potential mechanism for the impaired splenic B cell functions. Functionally, the splenic memory B cells from cirrhotic patients showed strong metabolic defects and reduced proliferation compared with those from healthy controls. Thus, liver cirrhosis extensively disturbs the splenic and peripheral B cell compartments, which may contribute to defective humoral immunity during liver cirrhosis. Nature Publishing Group UK 2020-10-24 /pmc/articles/PMC7585577/ /pubmed/33099582 http://dx.doi.org/10.1038/s41419-020-03060-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Huang, Man
Liu, Xiaoju
Ye, Haocheng
Zhao, Xin
Zhao, Juanjuan
Liu, Yang
He, Xiaomeng
Qu, Mengmeng
Pan, Jing
Hou, Baidong
Cheng, Yongqian
Liu, Zhenwen
Li, Zhiwei
Liu, Lei
Sun, Jian
Zhang, Shuye
Zhang, Zheng
Metabolic defects in splenic B cell compartments from patients with liver cirrhosis
title Metabolic defects in splenic B cell compartments from patients with liver cirrhosis
title_full Metabolic defects in splenic B cell compartments from patients with liver cirrhosis
title_fullStr Metabolic defects in splenic B cell compartments from patients with liver cirrhosis
title_full_unstemmed Metabolic defects in splenic B cell compartments from patients with liver cirrhosis
title_short Metabolic defects in splenic B cell compartments from patients with liver cirrhosis
title_sort metabolic defects in splenic b cell compartments from patients with liver cirrhosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585577/
https://www.ncbi.nlm.nih.gov/pubmed/33099582
http://dx.doi.org/10.1038/s41419-020-03060-1
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