Cargando…

Systemic Effects Induced by Hyperoxia in a Preclinical Model of Intra-abdominal Sepsis

Supplemental oxygen is a supportive treatment in patients with sepsis to balance tissue oxygen delivery and demand in the tissues. However, hyperoxia may induce some pathological effects. We sought to assess organ damage associated with hyperoxia and its correlation with the production of reactive o...

Descripción completa

Detalles Bibliográficos
Autores principales: García-Laorden, M. Isabel, Rodríguez-González, Raquel, Martín-Barrasa, José L., García-Hernández, Sonia, Ramos-Nuez, Ángela, González-García, H. Celeste, González-Martín, Jesús M., Kacmarek, Robert M., Villar, Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585649/
https://www.ncbi.nlm.nih.gov/pubmed/33122967
http://dx.doi.org/10.1155/2020/5101834
_version_ 1783599835766587392
author García-Laorden, M. Isabel
Rodríguez-González, Raquel
Martín-Barrasa, José L.
García-Hernández, Sonia
Ramos-Nuez, Ángela
González-García, H. Celeste
González-Martín, Jesús M.
Kacmarek, Robert M.
Villar, Jesús
author_facet García-Laorden, M. Isabel
Rodríguez-González, Raquel
Martín-Barrasa, José L.
García-Hernández, Sonia
Ramos-Nuez, Ángela
González-García, H. Celeste
González-Martín, Jesús M.
Kacmarek, Robert M.
Villar, Jesús
author_sort García-Laorden, M. Isabel
collection PubMed
description Supplemental oxygen is a supportive treatment in patients with sepsis to balance tissue oxygen delivery and demand in the tissues. However, hyperoxia may induce some pathological effects. We sought to assess organ damage associated with hyperoxia and its correlation with the production of reactive oxygen species (ROS) in a preclinical model of intra-abdominal sepsis. For this purpose, sepsis was induced in male, Sprague-Dawley rats by cecal ligation and puncture (CLP). We randomly assigned experimental animals to three groups: control (healthy animals), septic (CLP), and sham-septic (surgical intervention without CLP). At 18 h after CLP, septic (n = 39), sham-septic (n = 16), and healthy (n = 24) animals were placed within a sealed Plexiglas cage and randomly distributed into four groups for continuous treatment with 21%, 40%, 60%, or 100% oxygen for 24 h. At the end of the experimental period, we evaluated serum levels of cytokines, organ damage biomarkers, histological examination of brain and lung tissue, and ROS production in each surviving animal. We found that high oxygen concentrations increased IL-6 and biomarkers of organ damage levels in septic animals, although no relevant histopathological lung or brain damage was observed. Healthy rats had an increase in IL-6 and aspartate aminotransferase at high oxygen concentration. IL-6 levels, but not ROS levels, are correlated with markers of organ damage. In our study, the use of high oxygen concentrations in a clinically relevant model of intra-abdominal sepsis was associated with enhanced inflammation and organ damage. These findings were unrelated to ROS release into circulation. Hyperoxia could exacerbate sepsis-induced inflammation, and it could be by itself detrimental. Our study highlights the need of developing safer thresholds for oxygen therapy.
format Online
Article
Text
id pubmed-7585649
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-75856492020-10-28 Systemic Effects Induced by Hyperoxia in a Preclinical Model of Intra-abdominal Sepsis García-Laorden, M. Isabel Rodríguez-González, Raquel Martín-Barrasa, José L. García-Hernández, Sonia Ramos-Nuez, Ángela González-García, H. Celeste González-Martín, Jesús M. Kacmarek, Robert M. Villar, Jesús Mediators Inflamm Research Article Supplemental oxygen is a supportive treatment in patients with sepsis to balance tissue oxygen delivery and demand in the tissues. However, hyperoxia may induce some pathological effects. We sought to assess organ damage associated with hyperoxia and its correlation with the production of reactive oxygen species (ROS) in a preclinical model of intra-abdominal sepsis. For this purpose, sepsis was induced in male, Sprague-Dawley rats by cecal ligation and puncture (CLP). We randomly assigned experimental animals to three groups: control (healthy animals), septic (CLP), and sham-septic (surgical intervention without CLP). At 18 h after CLP, septic (n = 39), sham-septic (n = 16), and healthy (n = 24) animals were placed within a sealed Plexiglas cage and randomly distributed into four groups for continuous treatment with 21%, 40%, 60%, or 100% oxygen for 24 h. At the end of the experimental period, we evaluated serum levels of cytokines, organ damage biomarkers, histological examination of brain and lung tissue, and ROS production in each surviving animal. We found that high oxygen concentrations increased IL-6 and biomarkers of organ damage levels in septic animals, although no relevant histopathological lung or brain damage was observed. Healthy rats had an increase in IL-6 and aspartate aminotransferase at high oxygen concentration. IL-6 levels, but not ROS levels, are correlated with markers of organ damage. In our study, the use of high oxygen concentrations in a clinically relevant model of intra-abdominal sepsis was associated with enhanced inflammation and organ damage. These findings were unrelated to ROS release into circulation. Hyperoxia could exacerbate sepsis-induced inflammation, and it could be by itself detrimental. Our study highlights the need of developing safer thresholds for oxygen therapy. Hindawi 2020-10-15 /pmc/articles/PMC7585649/ /pubmed/33122967 http://dx.doi.org/10.1155/2020/5101834 Text en Copyright © 2020 M. Isabel García-Laorden et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
García-Laorden, M. Isabel
Rodríguez-González, Raquel
Martín-Barrasa, José L.
García-Hernández, Sonia
Ramos-Nuez, Ángela
González-García, H. Celeste
González-Martín, Jesús M.
Kacmarek, Robert M.
Villar, Jesús
Systemic Effects Induced by Hyperoxia in a Preclinical Model of Intra-abdominal Sepsis
title Systemic Effects Induced by Hyperoxia in a Preclinical Model of Intra-abdominal Sepsis
title_full Systemic Effects Induced by Hyperoxia in a Preclinical Model of Intra-abdominal Sepsis
title_fullStr Systemic Effects Induced by Hyperoxia in a Preclinical Model of Intra-abdominal Sepsis
title_full_unstemmed Systemic Effects Induced by Hyperoxia in a Preclinical Model of Intra-abdominal Sepsis
title_short Systemic Effects Induced by Hyperoxia in a Preclinical Model of Intra-abdominal Sepsis
title_sort systemic effects induced by hyperoxia in a preclinical model of intra-abdominal sepsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7585649/
https://www.ncbi.nlm.nih.gov/pubmed/33122967
http://dx.doi.org/10.1155/2020/5101834
work_keys_str_mv AT garcialaordenmisabel systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis
AT rodriguezgonzalezraquel systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis
AT martinbarrasajosel systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis
AT garciahernandezsonia systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis
AT ramosnuezangela systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis
AT gonzalezgarciahceleste systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis
AT gonzalezmartinjesusm systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis
AT kacmarekrobertm systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis
AT villarjesus systemiceffectsinducedbyhyperoxiainapreclinicalmodelofintraabdominalsepsis