Long-term single-cell passaging of human iPSC fully supports pluripotency and high-efficient trilineage differentiation capacity

OBJECTIVES: To establish a straightforward single-cell passaging cultivation method that enables high-quality maintenance of human induced pluripotent stem cells without the appearance of karyotypic abnormalities or loss of pluripotency. METHODS: Cells were kept in culture for over 50 passages, foll...

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Detalles Bibliográficos
Autores principales: Cruvinel, Estela, Ogusuku, Isabella, Cerioni, Rosanna, Rodrigues, Sirlene, Gonçalves, Jéssica, Góes, Maria Elisa, Alvim, Juliana Morais, Silva, Anderson Carlos, Lino, Vanesca de Souza, Boccardo, Enrique, Goulart, Ernesto, Pereira, Alexandre, Dariolli, Rafael, Valadares, Marcos, Biagi, Diogo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586033/
https://www.ncbi.nlm.nih.gov/pubmed/33149912
http://dx.doi.org/10.1177/2050312120966456

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