Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis

Firstly, optimal parameters of crude polysaccharide from Buddleja officinalis were obtained as follows: ratio of water to raw material of 26 : 1, ultrasonic power of 240 W, ultrasonic time of 45 min, and ultrasonic temperature of 62°C. Secondly, acidic polysaccharide (APBOM) from Buddleja officinali...

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Autores principales: Yan, Xiaoteng, Yan, Zhuan, Xiong, Qingping, Liu, Gaoqin, Zhu, Juanjuan, Lu, Peirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586156/
https://www.ncbi.nlm.nih.gov/pubmed/33123208
http://dx.doi.org/10.1155/2020/5175138
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author Yan, Xiaoteng
Yan, Zhuan
Xiong, Qingping
Liu, Gaoqin
Zhu, Juanjuan
Lu, Peirong
author_facet Yan, Xiaoteng
Yan, Zhuan
Xiong, Qingping
Liu, Gaoqin
Zhu, Juanjuan
Lu, Peirong
author_sort Yan, Xiaoteng
collection PubMed
description Firstly, optimal parameters of crude polysaccharide from Buddleja officinalis were obtained as follows: ratio of water to raw material of 26 : 1, ultrasonic power of 240 W, ultrasonic time of 45 min, and ultrasonic temperature of 62°C. Secondly, acidic polysaccharide (APBOM) from Buddleja officinalis was successfully acquired with the yield of 9.57% by using DEAE-52 cellulose and Sephadex G-100 gel column chromatography. Then, we found that total polysaccharide content of APBOM was 94.37% with a sulfuric acid group of 1.68%, uronic acid content of 17.41%, and average molecular weight of 165.4 kDa. Finally, APBOM was confirmed to have significant antiangiogenic effects.
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spelling pubmed-75861562020-10-28 Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis Yan, Xiaoteng Yan, Zhuan Xiong, Qingping Liu, Gaoqin Zhu, Juanjuan Lu, Peirong Evid Based Complement Alternat Med Research Article Firstly, optimal parameters of crude polysaccharide from Buddleja officinalis were obtained as follows: ratio of water to raw material of 26 : 1, ultrasonic power of 240 W, ultrasonic time of 45 min, and ultrasonic temperature of 62°C. Secondly, acidic polysaccharide (APBOM) from Buddleja officinalis was successfully acquired with the yield of 9.57% by using DEAE-52 cellulose and Sephadex G-100 gel column chromatography. Then, we found that total polysaccharide content of APBOM was 94.37% with a sulfuric acid group of 1.68%, uronic acid content of 17.41%, and average molecular weight of 165.4 kDa. Finally, APBOM was confirmed to have significant antiangiogenic effects. Hindawi 2020-10-17 /pmc/articles/PMC7586156/ /pubmed/33123208 http://dx.doi.org/10.1155/2020/5175138 Text en Copyright © 2020 Xiaoteng Yan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yan, Xiaoteng
Yan, Zhuan
Xiong, Qingping
Liu, Gaoqin
Zhu, Juanjuan
Lu, Peirong
Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis
title Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis
title_full Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis
title_fullStr Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis
title_full_unstemmed Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis
title_short Extraction, Purification, Characterization, and Antiangiogenic Activity of Acidic Polysaccharide from Buddleja officinalis
title_sort extraction, purification, characterization, and antiangiogenic activity of acidic polysaccharide from buddleja officinalis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586156/
https://www.ncbi.nlm.nih.gov/pubmed/33123208
http://dx.doi.org/10.1155/2020/5175138
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