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Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus
It has been proven that vitamin D was decreased and function of circulating endothelial progenitor cells (EPCs) was injured in systemic lupus erythematosus (SLE) patients. However, the effect of vitamin D on the function of EPCs in vitro and its mechanism need further study. Therefore, we investigat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586170/ https://www.ncbi.nlm.nih.gov/pubmed/33123377 http://dx.doi.org/10.1155/2020/6802562 |
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author | Huang, Zhenhua Liu, Lixiang Huang, Shufen Li, Jianbo Feng, Shaozhen Huang, Naya Ai, Zhen Long, Weiqing Jiang, Lanping |
author_facet | Huang, Zhenhua Liu, Lixiang Huang, Shufen Li, Jianbo Feng, Shaozhen Huang, Naya Ai, Zhen Long, Weiqing Jiang, Lanping |
author_sort | Huang, Zhenhua |
collection | PubMed |
description | It has been proven that vitamin D was decreased and function of circulating endothelial progenitor cells (EPCs) was injured in systemic lupus erythematosus (SLE) patients. However, the effect of vitamin D on the function of EPCs in vitro and its mechanism need further study. Therefore, we investigated whether vitamin D improved the function of EPCs in vitro. The peripheral blood mononuclear cells of the participants were isolated from SLE patients and control subjects and cultured to EPCs. After the EPCs were treated with vitamin D (1,25-(OH)(2)D(3)), we evaluated the number, migratory and proliferative activities, and nitric oxide (NO) production of EPCs in vitro and detected vascular endothelial function by flow-mediated dilatation (FMD). We found that vitamin D in a dose-dependent manner improved number and migratory and proliferative activities of EPCs from SLE patients. Additionally, vitamin D upregulated NO production from EPCs in vitro. A significant correlation between the FMD and plasma NO level was found. There was also a correlation between number, migration, and proliferation of EPCs and NO production. Thus, the present findings indicated that vitamin D improved the function of EPCs from SLE patients via NO secretion. |
format | Online Article Text |
id | pubmed-7586170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-75861702020-10-28 Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus Huang, Zhenhua Liu, Lixiang Huang, Shufen Li, Jianbo Feng, Shaozhen Huang, Naya Ai, Zhen Long, Weiqing Jiang, Lanping Cardiol Res Pract Research Article It has been proven that vitamin D was decreased and function of circulating endothelial progenitor cells (EPCs) was injured in systemic lupus erythematosus (SLE) patients. However, the effect of vitamin D on the function of EPCs in vitro and its mechanism need further study. Therefore, we investigated whether vitamin D improved the function of EPCs in vitro. The peripheral blood mononuclear cells of the participants were isolated from SLE patients and control subjects and cultured to EPCs. After the EPCs were treated with vitamin D (1,25-(OH)(2)D(3)), we evaluated the number, migratory and proliferative activities, and nitric oxide (NO) production of EPCs in vitro and detected vascular endothelial function by flow-mediated dilatation (FMD). We found that vitamin D in a dose-dependent manner improved number and migratory and proliferative activities of EPCs from SLE patients. Additionally, vitamin D upregulated NO production from EPCs in vitro. A significant correlation between the FMD and plasma NO level was found. There was also a correlation between number, migration, and proliferation of EPCs and NO production. Thus, the present findings indicated that vitamin D improved the function of EPCs from SLE patients via NO secretion. Hindawi 2020-10-16 /pmc/articles/PMC7586170/ /pubmed/33123377 http://dx.doi.org/10.1155/2020/6802562 Text en Copyright © 2020 Zhenhua Huang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Huang, Zhenhua Liu, Lixiang Huang, Shufen Li, Jianbo Feng, Shaozhen Huang, Naya Ai, Zhen Long, Weiqing Jiang, Lanping Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus |
title | Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus |
title_full | Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus |
title_fullStr | Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus |
title_full_unstemmed | Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus |
title_short | Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus |
title_sort | vitamin d (1,25-(oh)(2)d(3)) improves endothelial progenitor cells function via enhanced no secretion in systemic lupus erythematosus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586170/ https://www.ncbi.nlm.nih.gov/pubmed/33123377 http://dx.doi.org/10.1155/2020/6802562 |
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