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Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus

It has been proven that vitamin D was decreased and function of circulating endothelial progenitor cells (EPCs) was injured in systemic lupus erythematosus (SLE) patients. However, the effect of vitamin D on the function of EPCs in vitro and its mechanism need further study. Therefore, we investigat...

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Autores principales: Huang, Zhenhua, Liu, Lixiang, Huang, Shufen, Li, Jianbo, Feng, Shaozhen, Huang, Naya, Ai, Zhen, Long, Weiqing, Jiang, Lanping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586170/
https://www.ncbi.nlm.nih.gov/pubmed/33123377
http://dx.doi.org/10.1155/2020/6802562
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author Huang, Zhenhua
Liu, Lixiang
Huang, Shufen
Li, Jianbo
Feng, Shaozhen
Huang, Naya
Ai, Zhen
Long, Weiqing
Jiang, Lanping
author_facet Huang, Zhenhua
Liu, Lixiang
Huang, Shufen
Li, Jianbo
Feng, Shaozhen
Huang, Naya
Ai, Zhen
Long, Weiqing
Jiang, Lanping
author_sort Huang, Zhenhua
collection PubMed
description It has been proven that vitamin D was decreased and function of circulating endothelial progenitor cells (EPCs) was injured in systemic lupus erythematosus (SLE) patients. However, the effect of vitamin D on the function of EPCs in vitro and its mechanism need further study. Therefore, we investigated whether vitamin D improved the function of EPCs in vitro. The peripheral blood mononuclear cells of the participants were isolated from SLE patients and control subjects and cultured to EPCs. After the EPCs were treated with vitamin D (1,25-(OH)(2)D(3)), we evaluated the number, migratory and proliferative activities, and nitric oxide (NO) production of EPCs in vitro and detected vascular endothelial function by flow-mediated dilatation (FMD). We found that vitamin D in a dose-dependent manner improved number and migratory and proliferative activities of EPCs from SLE patients. Additionally, vitamin D upregulated NO production from EPCs in vitro. A significant correlation between the FMD and plasma NO level was found. There was also a correlation between number, migration, and proliferation of EPCs and NO production. Thus, the present findings indicated that vitamin D improved the function of EPCs from SLE patients via NO secretion.
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spelling pubmed-75861702020-10-28 Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus Huang, Zhenhua Liu, Lixiang Huang, Shufen Li, Jianbo Feng, Shaozhen Huang, Naya Ai, Zhen Long, Weiqing Jiang, Lanping Cardiol Res Pract Research Article It has been proven that vitamin D was decreased and function of circulating endothelial progenitor cells (EPCs) was injured in systemic lupus erythematosus (SLE) patients. However, the effect of vitamin D on the function of EPCs in vitro and its mechanism need further study. Therefore, we investigated whether vitamin D improved the function of EPCs in vitro. The peripheral blood mononuclear cells of the participants were isolated from SLE patients and control subjects and cultured to EPCs. After the EPCs were treated with vitamin D (1,25-(OH)(2)D(3)), we evaluated the number, migratory and proliferative activities, and nitric oxide (NO) production of EPCs in vitro and detected vascular endothelial function by flow-mediated dilatation (FMD). We found that vitamin D in a dose-dependent manner improved number and migratory and proliferative activities of EPCs from SLE patients. Additionally, vitamin D upregulated NO production from EPCs in vitro. A significant correlation between the FMD and plasma NO level was found. There was also a correlation between number, migration, and proliferation of EPCs and NO production. Thus, the present findings indicated that vitamin D improved the function of EPCs from SLE patients via NO secretion. Hindawi 2020-10-16 /pmc/articles/PMC7586170/ /pubmed/33123377 http://dx.doi.org/10.1155/2020/6802562 Text en Copyright © 2020 Zhenhua Huang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Zhenhua
Liu, Lixiang
Huang, Shufen
Li, Jianbo
Feng, Shaozhen
Huang, Naya
Ai, Zhen
Long, Weiqing
Jiang, Lanping
Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus
title Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus
title_full Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus
title_fullStr Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus
title_full_unstemmed Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus
title_short Vitamin D (1,25-(OH)(2)D(3)) Improves Endothelial Progenitor Cells Function via Enhanced NO Secretion in Systemic Lupus Erythematosus
title_sort vitamin d (1,25-(oh)(2)d(3)) improves endothelial progenitor cells function via enhanced no secretion in systemic lupus erythematosus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586170/
https://www.ncbi.nlm.nih.gov/pubmed/33123377
http://dx.doi.org/10.1155/2020/6802562
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