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A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood

Peripheral blood (PB) contains several types of stem/progenitor cells, including hematopoietic stem and endothelial progenitor cells. We identified a population positive for both the pluripotent surface marker SSEA-3 and leukocyte common antigen CD45 that comprises 0.04% ± 0.003% of the mononuclear...

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Autores principales: Sato, Tetsuya, Wakao, Shohei, Kushida, Yoshihiro, Tatsumi, Kazuki, Kitada, Masaaki, Abe, Takatsugu, Niizuma, Kuniyasu, Tominaga, Teiji, Kushimoto, Shigeki, Dezawa, Mari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586270/
https://www.ncbi.nlm.nih.gov/pubmed/32525407
http://dx.doi.org/10.1177/0963689720923574
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author Sato, Tetsuya
Wakao, Shohei
Kushida, Yoshihiro
Tatsumi, Kazuki
Kitada, Masaaki
Abe, Takatsugu
Niizuma, Kuniyasu
Tominaga, Teiji
Kushimoto, Shigeki
Dezawa, Mari
author_facet Sato, Tetsuya
Wakao, Shohei
Kushida, Yoshihiro
Tatsumi, Kazuki
Kitada, Masaaki
Abe, Takatsugu
Niizuma, Kuniyasu
Tominaga, Teiji
Kushimoto, Shigeki
Dezawa, Mari
author_sort Sato, Tetsuya
collection PubMed
description Peripheral blood (PB) contains several types of stem/progenitor cells, including hematopoietic stem and endothelial progenitor cells. We identified a population positive for both the pluripotent surface marker SSEA-3 and leukocyte common antigen CD45 that comprises 0.04% ± 0.003% of the mononuclear cells in human PB. The average size of the SSEA-3(+)/CD45(+) cells was 10.1 ± 0.3 µm and ∼22% were positive for CD105, a mesenchymal marker; ∼85% were positive for CD19, a B cell marker; and ∼94% were positive for HLA-DR, a major histocompatibility complex class II molecule relevant to antigen presentation. These SSEA-3(+)/CD45(+) cells expressed the pluripotency markers Nanog, Oct3/4, and Sox2, as well as sphingosine-1-phosphate (S1P) receptor 2, and migrated toward S1P, although their adherence and proliferative activities in vitro were low. They expressed NeuN at 7 d, Pax7 and desmin at 7 d, and alpha-fetoprotein and cytokeratin-19 at 3 d when supplied to mouse damaged tissues of the brain, skeletal muscle and liver, respectively, suggesting the ability to spontaneously differentiate into triploblastic lineages compatible to the tissue microenvironment. Multilineage-differentiating stress enduring (Muse) cells, identified as SSEA-3(+) in tissues such as the bone marrow and organ connective tissues, express pluripotency markers, migrate to sites of damage via the S1P-S1P receptor 2 system, and differentiate spontaneously into tissue-compatible cells after homing to the damaged tissue where they participate in tissue repair. After the onset of acute myocardial infarction and stroke, patients are reported to have an increase in the number of SSEA-3(+) cells in the PB. The SSEA-3(+)/CD45(+) cells in the PB showed similarity to tissue-Muse cells, although with difference in surface marker expression and cellular properties. Thus, these findings suggest that human PB contains a subset of cells that are distinct from known stem/progenitor cells, and that CD45(+)-mononuclear cells in the PB comprise a novel subpopulation of cells that express pluripotency markers.
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spelling pubmed-75862702020-11-03 A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood Sato, Tetsuya Wakao, Shohei Kushida, Yoshihiro Tatsumi, Kazuki Kitada, Masaaki Abe, Takatsugu Niizuma, Kuniyasu Tominaga, Teiji Kushimoto, Shigeki Dezawa, Mari Cell Transplant Original Article Peripheral blood (PB) contains several types of stem/progenitor cells, including hematopoietic stem and endothelial progenitor cells. We identified a population positive for both the pluripotent surface marker SSEA-3 and leukocyte common antigen CD45 that comprises 0.04% ± 0.003% of the mononuclear cells in human PB. The average size of the SSEA-3(+)/CD45(+) cells was 10.1 ± 0.3 µm and ∼22% were positive for CD105, a mesenchymal marker; ∼85% were positive for CD19, a B cell marker; and ∼94% were positive for HLA-DR, a major histocompatibility complex class II molecule relevant to antigen presentation. These SSEA-3(+)/CD45(+) cells expressed the pluripotency markers Nanog, Oct3/4, and Sox2, as well as sphingosine-1-phosphate (S1P) receptor 2, and migrated toward S1P, although their adherence and proliferative activities in vitro were low. They expressed NeuN at 7 d, Pax7 and desmin at 7 d, and alpha-fetoprotein and cytokeratin-19 at 3 d when supplied to mouse damaged tissues of the brain, skeletal muscle and liver, respectively, suggesting the ability to spontaneously differentiate into triploblastic lineages compatible to the tissue microenvironment. Multilineage-differentiating stress enduring (Muse) cells, identified as SSEA-3(+) in tissues such as the bone marrow and organ connective tissues, express pluripotency markers, migrate to sites of damage via the S1P-S1P receptor 2 system, and differentiate spontaneously into tissue-compatible cells after homing to the damaged tissue where they participate in tissue repair. After the onset of acute myocardial infarction and stroke, patients are reported to have an increase in the number of SSEA-3(+) cells in the PB. The SSEA-3(+)/CD45(+) cells in the PB showed similarity to tissue-Muse cells, although with difference in surface marker expression and cellular properties. Thus, these findings suggest that human PB contains a subset of cells that are distinct from known stem/progenitor cells, and that CD45(+)-mononuclear cells in the PB comprise a novel subpopulation of cells that express pluripotency markers. SAGE Publications 2020-06-11 /pmc/articles/PMC7586270/ /pubmed/32525407 http://dx.doi.org/10.1177/0963689720923574 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Sato, Tetsuya
Wakao, Shohei
Kushida, Yoshihiro
Tatsumi, Kazuki
Kitada, Masaaki
Abe, Takatsugu
Niizuma, Kuniyasu
Tominaga, Teiji
Kushimoto, Shigeki
Dezawa, Mari
A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood
title A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood
title_full A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood
title_fullStr A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood
title_full_unstemmed A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood
title_short A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood
title_sort novel type of stem cells double-positive for ssea-3 and cd45 in human peripheral blood
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586270/
https://www.ncbi.nlm.nih.gov/pubmed/32525407
http://dx.doi.org/10.1177/0963689720923574
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