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Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola

ObjectiveTreponema denticola is involved in ‘chronic’ periodontitis pathogenesis. The mechanism underlying the regulation of the expression of its virulence factors, such as major surface protein (Msp) and prolyl-phenylalanine specific protease (dentilisin) is yet to be clarified. We determined the...

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Autores principales: Arai, Yuki, Kikuchi, Yuichiro, Okamoto-Shibayama, Kazuko, Kokubu, Eitoyo, Shintani, Seikou, Ishihara, Kazuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586716/
https://www.ncbi.nlm.nih.gov/pubmed/33149843
http://dx.doi.org/10.1080/20002297.2020.1829404
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author Arai, Yuki
Kikuchi, Yuichiro
Okamoto-Shibayama, Kazuko
Kokubu, Eitoyo
Shintani, Seikou
Ishihara, Kazuyuki
author_facet Arai, Yuki
Kikuchi, Yuichiro
Okamoto-Shibayama, Kazuko
Kokubu, Eitoyo
Shintani, Seikou
Ishihara, Kazuyuki
author_sort Arai, Yuki
collection PubMed
description ObjectiveTreponema denticola is involved in ‘chronic’ periodontitis pathogenesis. The mechanism underlying the regulation of the expression of its virulence factors, such as major surface protein (Msp) and prolyl-phenylalanine specific protease (dentilisin) is yet to be clarified. We determined the gene expression profiles of Msp- and dentilisin-deficient mutants of T. denticola to identify the regulation network of gene expression concomitant with the inactivation of these virulence genes. Methods Gene expression profiles of T. denticola ATCC 35405 (wild type), dentilisin-deficient mutant K1, and msp-deficient mutant DMSP3 were determined using DNA microarray analysis and quantitative real-time reverse transcription PCR (qRT-PCR). Msp and dentilisin protein levels were determined by immunoblotting and proteolytic activity assays. Results In addition to several differentially expressed genes, dentilisin expression was reduced in DMSP3; msp expression was significantly reduced in K1 (p < 0.05), both at the gene and protein levels. To identify the regulatory system involved, the expression levels of the potential regulators whose expression showed changes in the mutants were evaluated using qRT-PCR. Transcriptional regulators TDE_0127 and TDE_0814 were upregulated in K1, and the potential repressor, TDE_0344, was elevated in DMSP3. Conclusions Dentilisin and Msp expression were interrelated, and gene expression regulators, such as TDE_0127, may be involved in their regulation.
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spelling pubmed-75867162020-11-03 Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola Arai, Yuki Kikuchi, Yuichiro Okamoto-Shibayama, Kazuko Kokubu, Eitoyo Shintani, Seikou Ishihara, Kazuyuki J Oral Microbiol Original Article ObjectiveTreponema denticola is involved in ‘chronic’ periodontitis pathogenesis. The mechanism underlying the regulation of the expression of its virulence factors, such as major surface protein (Msp) and prolyl-phenylalanine specific protease (dentilisin) is yet to be clarified. We determined the gene expression profiles of Msp- and dentilisin-deficient mutants of T. denticola to identify the regulation network of gene expression concomitant with the inactivation of these virulence genes. Methods Gene expression profiles of T. denticola ATCC 35405 (wild type), dentilisin-deficient mutant K1, and msp-deficient mutant DMSP3 were determined using DNA microarray analysis and quantitative real-time reverse transcription PCR (qRT-PCR). Msp and dentilisin protein levels were determined by immunoblotting and proteolytic activity assays. Results In addition to several differentially expressed genes, dentilisin expression was reduced in DMSP3; msp expression was significantly reduced in K1 (p < 0.05), both at the gene and protein levels. To identify the regulatory system involved, the expression levels of the potential regulators whose expression showed changes in the mutants were evaluated using qRT-PCR. Transcriptional regulators TDE_0127 and TDE_0814 were upregulated in K1, and the potential repressor, TDE_0344, was elevated in DMSP3. Conclusions Dentilisin and Msp expression were interrelated, and gene expression regulators, such as TDE_0127, may be involved in their regulation. Taylor & Francis 2020-10-11 /pmc/articles/PMC7586716/ /pubmed/33149843 http://dx.doi.org/10.1080/20002297.2020.1829404 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Arai, Yuki
Kikuchi, Yuichiro
Okamoto-Shibayama, Kazuko
Kokubu, Eitoyo
Shintani, Seikou
Ishihara, Kazuyuki
Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola
title Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola
title_full Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola
title_fullStr Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola
title_full_unstemmed Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola
title_short Investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in Treponema denticola
title_sort investigation of the potential regulator proteins associated with the expression of major surface protein and dentilisin in treponema denticola
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7586716/
https://www.ncbi.nlm.nih.gov/pubmed/33149843
http://dx.doi.org/10.1080/20002297.2020.1829404
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