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X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components

Thanks to its non-invasive nature, X-ray phase contrast tomography is a very versatile imaging tool for biomedical studies. In contrast, histology is a well-established method, though having its limitations: it requires extensive sample preparation and it is quite time consuming. Therefore, the deve...

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Autores principales: Longo, E., Sancey, L., Flenner, S., Kubec, A., Bonnin, A., David, C., Müller, M., Greving, I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587279/
https://www.ncbi.nlm.nih.gov/pubmed/33149967
http://dx.doi.org/10.1364/BOE.396695
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author Longo, E.
Sancey, L.
Flenner, S.
Kubec, A.
Bonnin, A.
David, C.
Müller, M.
Greving, I.
author_facet Longo, E.
Sancey, L.
Flenner, S.
Kubec, A.
Bonnin, A.
David, C.
Müller, M.
Greving, I.
author_sort Longo, E.
collection PubMed
description Thanks to its non-invasive nature, X-ray phase contrast tomography is a very versatile imaging tool for biomedical studies. In contrast, histology is a well-established method, though having its limitations: it requires extensive sample preparation and it is quite time consuming. Therefore, the development of nano-imaging techniques for studying anatomic details at the cellular level is gaining more and more importance. In this article, full field transmission X-ray nanotomography is used in combination with Zernike phase contrast to image millimeter sized unstained tissue samples at high spatial resolution. The regions of interest (ROI) scans of different tissues were obtained from mouse kidney, spleen and mammalian carcinoma. Thanks to the relatively large field of view and effective pixel sizes down to 36 nm, this 3D approach enabled the visualization of the specific morphology of each tissue type without staining or complex sample preparation. As a proof of concept technique, we show that the high-quality images even permitted the 3D segmentation of multiple structures down to a sub-cellular level. Using stitching techniques, volumes larger than the field of view are accessible. This method can lead to a deeper understanding of the organs’ nano-anatomy, filling the resolution gap between histology and transmission electron microscopy.
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spelling pubmed-75872792020-11-03 X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components Longo, E. Sancey, L. Flenner, S. Kubec, A. Bonnin, A. David, C. Müller, M. Greving, I. Biomed Opt Express Article Thanks to its non-invasive nature, X-ray phase contrast tomography is a very versatile imaging tool for biomedical studies. In contrast, histology is a well-established method, though having its limitations: it requires extensive sample preparation and it is quite time consuming. Therefore, the development of nano-imaging techniques for studying anatomic details at the cellular level is gaining more and more importance. In this article, full field transmission X-ray nanotomography is used in combination with Zernike phase contrast to image millimeter sized unstained tissue samples at high spatial resolution. The regions of interest (ROI) scans of different tissues were obtained from mouse kidney, spleen and mammalian carcinoma. Thanks to the relatively large field of view and effective pixel sizes down to 36 nm, this 3D approach enabled the visualization of the specific morphology of each tissue type without staining or complex sample preparation. As a proof of concept technique, we show that the high-quality images even permitted the 3D segmentation of multiple structures down to a sub-cellular level. Using stitching techniques, volumes larger than the field of view are accessible. This method can lead to a deeper understanding of the organs’ nano-anatomy, filling the resolution gap between histology and transmission electron microscopy. Optical Society of America 2020-09-10 /pmc/articles/PMC7587279/ /pubmed/33149967 http://dx.doi.org/10.1364/BOE.396695 Text en Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) . Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
spellingShingle Article
Longo, E.
Sancey, L.
Flenner, S.
Kubec, A.
Bonnin, A.
David, C.
Müller, M.
Greving, I.
X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components
title X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components
title_full X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components
title_fullStr X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components
title_full_unstemmed X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components
title_short X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components
title_sort x-ray zernike phase contrast tomography: 3d roi visualization of mm-sized mice organ tissues down to sub-cellular components
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587279/
https://www.ncbi.nlm.nih.gov/pubmed/33149967
http://dx.doi.org/10.1364/BOE.396695
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