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Mass Spectrometric Analysis of Antibody—Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization

Electrospray mass spectrometry is applied to determine apparent binding energies and quasi equilibrium dissociation constants of immune complex dissociation reactions in the gas phase. Myoglobin, a natural protein-ligand complex, has been used to develop the procedure which starts from determining m...

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Detalles Bibliográficos
Autores principales:	Danquah, Bright D., Opuni, Kwabena F. M., Roewer, Claudia, Koy, Cornelia, Glocker, Michael O.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587528/ https://www.ncbi.nlm.nih.gov/pubmed/33080923 http://dx.doi.org/10.3390/molecules25204776

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