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Bioactive Flavonoid Glycosides and HPLC and UPLC Quantification of Commercial Astragali Complanati Semen

Eleven compounds, including nine known flavonoid glycosides (1–4, 6–8, and 10–11), one isoflavone glycoside (5), and a glansreginic acid (9), were isolated from the 80% ethanol extract of commercial Astragali Complanati Semen (ACS). All chemical structures were determined by spectroscopic analyses,...

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Autores principales: Kuo, Jenny Chun-Ling, Zhang, Li-Jie, Huang, Hung-Tse, Liaw, Chia-Ching, Lin, Zhi-Hu, Liu, Min, Kuo, Yao-Haur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587559/
https://www.ncbi.nlm.nih.gov/pubmed/33081333
http://dx.doi.org/10.3390/molecules25204762
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author Kuo, Jenny Chun-Ling
Zhang, Li-Jie
Huang, Hung-Tse
Liaw, Chia-Ching
Lin, Zhi-Hu
Liu, Min
Kuo, Yao-Haur
author_facet Kuo, Jenny Chun-Ling
Zhang, Li-Jie
Huang, Hung-Tse
Liaw, Chia-Ching
Lin, Zhi-Hu
Liu, Min
Kuo, Yao-Haur
author_sort Kuo, Jenny Chun-Ling
collection PubMed
description Eleven compounds, including nine known flavonoid glycosides (1–4, 6–8, and 10–11), one isoflavone glycoside (5), and a glansreginic acid (9), were isolated from the 80% ethanol extract of commercial Astragali Complanati Semen (ACS). All chemical structures were determined by spectroscopic analyses, including 1D and 2D NMR. Compounds 2, 4, 5, 6, 9, and 10 were isolated and identified from the title plant for the first time. Biological evaluation revealed that all the isolates showed promising anti-NO production, and 1, 2, 3, and 8 were more potent in antioxidant activity than vitamin E. The major peaks in the UPLC and HPLC profiles identified their chemical structures by comparing their retention time and UV spectra with those of the reference substances. Furthermore, nine of the eleven samples collected from North, Middle, and South regions of Taiwan possessed similar HPLC fingerprints and were identified as Astragali Complanati Semen, whereas the other two samples from southern Taiwan would be the adulterants due to the different fingerprinting patterns. In addition, an HPLC-UV method was employed to determine the content of target compound complanatuside (11) with good linear regression (R(2) = 0.9998) for ACS in the Taiwanese market. Of the isolates, flavonol glycosides 1 and 3 were the major peaks in HPLC/UPLC, and showed more potent antioxidant and anti-NO production activities than that of 11, revealing that these compounds can be the available agents for the quality control of ACS.
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spelling pubmed-75875592020-10-29 Bioactive Flavonoid Glycosides and HPLC and UPLC Quantification of Commercial Astragali Complanati Semen Kuo, Jenny Chun-Ling Zhang, Li-Jie Huang, Hung-Tse Liaw, Chia-Ching Lin, Zhi-Hu Liu, Min Kuo, Yao-Haur Molecules Article Eleven compounds, including nine known flavonoid glycosides (1–4, 6–8, and 10–11), one isoflavone glycoside (5), and a glansreginic acid (9), were isolated from the 80% ethanol extract of commercial Astragali Complanati Semen (ACS). All chemical structures were determined by spectroscopic analyses, including 1D and 2D NMR. Compounds 2, 4, 5, 6, 9, and 10 were isolated and identified from the title plant for the first time. Biological evaluation revealed that all the isolates showed promising anti-NO production, and 1, 2, 3, and 8 were more potent in antioxidant activity than vitamin E. The major peaks in the UPLC and HPLC profiles identified their chemical structures by comparing their retention time and UV spectra with those of the reference substances. Furthermore, nine of the eleven samples collected from North, Middle, and South regions of Taiwan possessed similar HPLC fingerprints and were identified as Astragali Complanati Semen, whereas the other two samples from southern Taiwan would be the adulterants due to the different fingerprinting patterns. In addition, an HPLC-UV method was employed to determine the content of target compound complanatuside (11) with good linear regression (R(2) = 0.9998) for ACS in the Taiwanese market. Of the isolates, flavonol glycosides 1 and 3 were the major peaks in HPLC/UPLC, and showed more potent antioxidant and anti-NO production activities than that of 11, revealing that these compounds can be the available agents for the quality control of ACS. MDPI 2020-10-16 /pmc/articles/PMC7587559/ /pubmed/33081333 http://dx.doi.org/10.3390/molecules25204762 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kuo, Jenny Chun-Ling
Zhang, Li-Jie
Huang, Hung-Tse
Liaw, Chia-Ching
Lin, Zhi-Hu
Liu, Min
Kuo, Yao-Haur
Bioactive Flavonoid Glycosides and HPLC and UPLC Quantification of Commercial Astragali Complanati Semen
title Bioactive Flavonoid Glycosides and HPLC and UPLC Quantification of Commercial Astragali Complanati Semen
title_full Bioactive Flavonoid Glycosides and HPLC and UPLC Quantification of Commercial Astragali Complanati Semen
title_fullStr Bioactive Flavonoid Glycosides and HPLC and UPLC Quantification of Commercial Astragali Complanati Semen
title_full_unstemmed Bioactive Flavonoid Glycosides and HPLC and UPLC Quantification of Commercial Astragali Complanati Semen
title_short Bioactive Flavonoid Glycosides and HPLC and UPLC Quantification of Commercial Astragali Complanati Semen
title_sort bioactive flavonoid glycosides and hplc and uplc quantification of commercial astragali complanati semen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587559/
https://www.ncbi.nlm.nih.gov/pubmed/33081333
http://dx.doi.org/10.3390/molecules25204762
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