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Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin

The present study aimed to evaluate the immunopotentiating effect of plant-derived soyasaponin and its immunogenicity in chickens challenged with Newcastle disease virus (NDV). Soyasaponin was extracted from soybean seeds and detected using the phytochemical tests, followed by quantification through...

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Autores principales: Naveed, Ghania, Ehtisham-ul-Haque, Syed, Khan, Iahtasham, Rahman, Sajjad ur, Anam, Sidra, Usman, Muhammad, Shakir, Muhammad Zulqarnain, Naveed, Ahsan, Abbas, Ghazanfar, Anjum, Faisal Rasheed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587664/
https://www.ncbi.nlm.nih.gov/pubmed/32241472
http://dx.doi.org/10.1016/j.psj.2019.11.050
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author Naveed, Ghania
Ehtisham-ul-Haque, Syed
Khan, Iahtasham
Rahman, Sajjad ur
Anam, Sidra
Usman, Muhammad
Shakir, Muhammad Zulqarnain
Naveed, Ahsan
Abbas, Ghazanfar
Anjum, Faisal Rasheed
author_facet Naveed, Ghania
Ehtisham-ul-Haque, Syed
Khan, Iahtasham
Rahman, Sajjad ur
Anam, Sidra
Usman, Muhammad
Shakir, Muhammad Zulqarnain
Naveed, Ahsan
Abbas, Ghazanfar
Anjum, Faisal Rasheed
author_sort Naveed, Ghania
collection PubMed
description The present study aimed to evaluate the immunopotentiating effect of plant-derived soyasaponin and its immunogenicity in chickens challenged with Newcastle disease virus (NDV). Soyasaponin was extracted from soybean seeds and detected using the phytochemical tests, followed by quantification through the dry-weight method. One-day-old broiler chicks (n = 90) were divided into 3 groups, named as A, B, and C. Group A birds were orally administrated with soyasaponin (5 mg/kg), followed by immunization with inactivated ND vaccine intramuscularly (IM), whereas group B birds were vaccinated with inactivated ND vaccine alone. Group C birds were kept unvaccinated. A booster dose on day 21 was also administered IM to group A and B birds. At day 35, all 3 groups were challenged with NDV. To determine the immunogenicity potential of soyasaponin, antibody titer was measured using the hemagglutination inhibition test before and after the NDV challenge. Histochemical examination was performed to determine the pathological changes associated with NDV infection. Foam formation and hemolytic activity confirmed the presence of saponin in soya bean extract. Group A birds showed a higher antibody response compared with group B and C birds. The disease challenge study showed that soyasaponin-adjuvanted NDV vaccine provided complete protection to group A birds against ND. Moreover, no side effects of soyasaponin were observed on the growth performance of birds during the experiment. Therefore, we can conclude that soyasaponin is a potential immunogenic agent and therefore could be a promising candidate to launch a protective humoral response against ND in chickens.
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spelling pubmed-75876642020-10-27 Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin Naveed, Ghania Ehtisham-ul-Haque, Syed Khan, Iahtasham Rahman, Sajjad ur Anam, Sidra Usman, Muhammad Shakir, Muhammad Zulqarnain Naveed, Ahsan Abbas, Ghazanfar Anjum, Faisal Rasheed Poult Sci Immunology, Health and Disease The present study aimed to evaluate the immunopotentiating effect of plant-derived soyasaponin and its immunogenicity in chickens challenged with Newcastle disease virus (NDV). Soyasaponin was extracted from soybean seeds and detected using the phytochemical tests, followed by quantification through the dry-weight method. One-day-old broiler chicks (n = 90) were divided into 3 groups, named as A, B, and C. Group A birds were orally administrated with soyasaponin (5 mg/kg), followed by immunization with inactivated ND vaccine intramuscularly (IM), whereas group B birds were vaccinated with inactivated ND vaccine alone. Group C birds were kept unvaccinated. A booster dose on day 21 was also administered IM to group A and B birds. At day 35, all 3 groups were challenged with NDV. To determine the immunogenicity potential of soyasaponin, antibody titer was measured using the hemagglutination inhibition test before and after the NDV challenge. Histochemical examination was performed to determine the pathological changes associated with NDV infection. Foam formation and hemolytic activity confirmed the presence of saponin in soya bean extract. Group A birds showed a higher antibody response compared with group B and C birds. The disease challenge study showed that soyasaponin-adjuvanted NDV vaccine provided complete protection to group A birds against ND. Moreover, no side effects of soyasaponin were observed on the growth performance of birds during the experiment. Therefore, we can conclude that soyasaponin is a potential immunogenic agent and therefore could be a promising candidate to launch a protective humoral response against ND in chickens. Elsevier 2020-02-27 /pmc/articles/PMC7587664/ /pubmed/32241472 http://dx.doi.org/10.1016/j.psj.2019.11.050 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Immunology, Health and Disease
Naveed, Ghania
Ehtisham-ul-Haque, Syed
Khan, Iahtasham
Rahman, Sajjad ur
Anam, Sidra
Usman, Muhammad
Shakir, Muhammad Zulqarnain
Naveed, Ahsan
Abbas, Ghazanfar
Anjum, Faisal Rasheed
Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin
title Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin
title_full Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin
title_fullStr Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin
title_full_unstemmed Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin
title_short Enhancement in humoral response against inactivated Newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin
title_sort enhancement in humoral response against inactivated newcastle disease vaccine in broiler chickens administered orally with plant-derived soyasaponin
topic Immunology, Health and Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587664/
https://www.ncbi.nlm.nih.gov/pubmed/32241472
http://dx.doi.org/10.1016/j.psj.2019.11.050
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