Cargando…

Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers

This research investigated effects of dietary β-sitosterol addition at different levels on serum lipid levels, immune function, oxidative status, and intestinal morphology in broilers. One-day-old broiler chicks were allocated to 5 groups of 6 replicates. Chickens in the 5 groups were fed a basal di...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheng, Yefei, Chen, Yueping, Li, Jun, Qu, Hengman, Zhao, Yurui, Wen, Chao, Zhou, Yanmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587718/
https://www.ncbi.nlm.nih.gov/pubmed/32111314
http://dx.doi.org/10.1016/j.psj.2019.10.025
_version_ 1783600234026237952
author Cheng, Yefei
Chen, Yueping
Li, Jun
Qu, Hengman
Zhao, Yurui
Wen, Chao
Zhou, Yanmin
author_facet Cheng, Yefei
Chen, Yueping
Li, Jun
Qu, Hengman
Zhao, Yurui
Wen, Chao
Zhou, Yanmin
author_sort Cheng, Yefei
collection PubMed
description This research investigated effects of dietary β-sitosterol addition at different levels on serum lipid levels, immune function, oxidative status, and intestinal morphology in broilers. One-day-old broiler chicks were allocated to 5 groups of 6 replicates. Chickens in the 5 groups were fed a basal diet supplemented with 0 (control group), 40, 60, 80, and 100 mg/kg of β-sitosterol for 42 D, respectively. β-Sitosterol linearly decreased (P < 0.05) concentrations of serum total cholesterol, jejunal tumor necrosis factor α (TNF-α), and ileal interleukin 1β (IL-1β) and mRNA relative expressions levels of jejunal TLR4 and ileal MyD88, whereas it linearly increased (P < 0.05) contents of jejunal immunoglobulin G (IgG), ileal secreted IgA and glutathione, jejunal catalase activity and Nrf2 mRNA relative expression level, villus height (VH), and VH-to-crypt depth (CD) ratio (VH:CD) in the jejunum and ileum. Linear and quadratic increases (P < 0.05) in absolute and relative spleen weight were observed by dietary β-sitosterol, whereas malondialdehyde (MDA) concentration in the jejunum and ileum followed the opposite trend (P < 0.05). Compared with the control group, dietary β-sitosterol at higher than or equal to 60 mg/kg level decreased (P < 0.05) contents of serum total cholesterol, ileal MDA, and jejunal TLR4 mRNA relative expression level, whereas it increased (P < 0.05) absolute spleen weight and ileal glutathione content. Higher than or equal to 80 mg/kg level of β-sitosterol enhanced (P < 0.05) jejunal IgG concentration, VH, catalase activity, and Nrf2 relative expression level and ileal secreted IgA content, but reduced (P < 0.05) ileal IL-1β content and MyD88 mRNA relative expression level. β-Sitosterol addition at 60 and 80 mg/kg levels increased (P < 0.05) relative spleen weight, whereas it decreased (P < 0.05) jejunal MDA accumulation. Moreover, 100 mg/kg level of β-sitosterol reduced (P < 0.05) jejunal TNF-α level, but it increased (P < 0.05) VH in the jejunum and VH:CD in the jejunum and ileum. Accordingly, dietary β-sitosterol supplementation could regulate serum cholesterol level, promote immune function, and improve intestinal oxidative status and morphology in broilers.
format Online
Article
Text
id pubmed-7587718
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-75877182020-10-27 Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers Cheng, Yefei Chen, Yueping Li, Jun Qu, Hengman Zhao, Yurui Wen, Chao Zhou, Yanmin Poult Sci Metabolism and Nutrition This research investigated effects of dietary β-sitosterol addition at different levels on serum lipid levels, immune function, oxidative status, and intestinal morphology in broilers. One-day-old broiler chicks were allocated to 5 groups of 6 replicates. Chickens in the 5 groups were fed a basal diet supplemented with 0 (control group), 40, 60, 80, and 100 mg/kg of β-sitosterol for 42 D, respectively. β-Sitosterol linearly decreased (P < 0.05) concentrations of serum total cholesterol, jejunal tumor necrosis factor α (TNF-α), and ileal interleukin 1β (IL-1β) and mRNA relative expressions levels of jejunal TLR4 and ileal MyD88, whereas it linearly increased (P < 0.05) contents of jejunal immunoglobulin G (IgG), ileal secreted IgA and glutathione, jejunal catalase activity and Nrf2 mRNA relative expression level, villus height (VH), and VH-to-crypt depth (CD) ratio (VH:CD) in the jejunum and ileum. Linear and quadratic increases (P < 0.05) in absolute and relative spleen weight were observed by dietary β-sitosterol, whereas malondialdehyde (MDA) concentration in the jejunum and ileum followed the opposite trend (P < 0.05). Compared with the control group, dietary β-sitosterol at higher than or equal to 60 mg/kg level decreased (P < 0.05) contents of serum total cholesterol, ileal MDA, and jejunal TLR4 mRNA relative expression level, whereas it increased (P < 0.05) absolute spleen weight and ileal glutathione content. Higher than or equal to 80 mg/kg level of β-sitosterol enhanced (P < 0.05) jejunal IgG concentration, VH, catalase activity, and Nrf2 relative expression level and ileal secreted IgA content, but reduced (P < 0.05) ileal IL-1β content and MyD88 mRNA relative expression level. β-Sitosterol addition at 60 and 80 mg/kg levels increased (P < 0.05) relative spleen weight, whereas it decreased (P < 0.05) jejunal MDA accumulation. Moreover, 100 mg/kg level of β-sitosterol reduced (P < 0.05) jejunal TNF-α level, but it increased (P < 0.05) VH in the jejunum and VH:CD in the jejunum and ileum. Accordingly, dietary β-sitosterol supplementation could regulate serum cholesterol level, promote immune function, and improve intestinal oxidative status and morphology in broilers. Elsevier 2020-01-28 /pmc/articles/PMC7587718/ /pubmed/32111314 http://dx.doi.org/10.1016/j.psj.2019.10.025 Text en © 2019 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Metabolism and Nutrition
Cheng, Yefei
Chen, Yueping
Li, Jun
Qu, Hengman
Zhao, Yurui
Wen, Chao
Zhou, Yanmin
Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers
title Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers
title_full Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers
title_fullStr Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers
title_full_unstemmed Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers
title_short Dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers
title_sort dietary β-sitosterol regulates serum lipid level and improves immune function, antioxidant status, and intestinal morphology in broilers
topic Metabolism and Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587718/
https://www.ncbi.nlm.nih.gov/pubmed/32111314
http://dx.doi.org/10.1016/j.psj.2019.10.025
work_keys_str_mv AT chengyefei dietarybsitosterolregulatesserumlipidlevelandimprovesimmunefunctionantioxidantstatusandintestinalmorphologyinbroilers
AT chenyueping dietarybsitosterolregulatesserumlipidlevelandimprovesimmunefunctionantioxidantstatusandintestinalmorphologyinbroilers
AT lijun dietarybsitosterolregulatesserumlipidlevelandimprovesimmunefunctionantioxidantstatusandintestinalmorphologyinbroilers
AT quhengman dietarybsitosterolregulatesserumlipidlevelandimprovesimmunefunctionantioxidantstatusandintestinalmorphologyinbroilers
AT zhaoyurui dietarybsitosterolregulatesserumlipidlevelandimprovesimmunefunctionantioxidantstatusandintestinalmorphologyinbroilers
AT wenchao dietarybsitosterolregulatesserumlipidlevelandimprovesimmunefunctionantioxidantstatusandintestinalmorphologyinbroilers
AT zhouyanmin dietarybsitosterolregulatesserumlipidlevelandimprovesimmunefunctionantioxidantstatusandintestinalmorphologyinbroilers