Cargando…

Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods

The self-assembly of proteins is an essential process for a variety of cellular functions including cell respiration, mobility and division. On the other hand, protein or peptide misfolding and aggregation is related to the development of Parkinson’s disease and Alzheimer’s disease, among other aggr...

Descripción completa

Detalles Bibliográficos
Autores principales: Pignataro, María Florencia, Herrera, María Georgina, Dodero, Verónica Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587993/
https://www.ncbi.nlm.nih.gov/pubmed/33096797
http://dx.doi.org/10.3390/molecules25204854
_version_ 1783600287680823296
author Pignataro, María Florencia
Herrera, María Georgina
Dodero, Verónica Isabel
author_facet Pignataro, María Florencia
Herrera, María Georgina
Dodero, Verónica Isabel
author_sort Pignataro, María Florencia
collection PubMed
description The self-assembly of proteins is an essential process for a variety of cellular functions including cell respiration, mobility and division. On the other hand, protein or peptide misfolding and aggregation is related to the development of Parkinson’s disease and Alzheimer’s disease, among other aggregopathies. As a consequence, significant research efforts are directed towards the understanding of this process. In this review, we are focused on the use of UV-Visible Absorption Spectroscopy, Fluorescence Spectroscopy and Circular Dichroism to evaluate the self-organization of proteins and peptides in solution. These spectroscopic techniques are commonly available in most chemistry and biochemistry research laboratories, and together they are a powerful approach for initial as well as routine evaluation of protein and peptide self-assembly and aggregation under different environmental stimulus. Furthermore, these spectroscopic techniques are even suitable for studying complex systems like those in the food industry or pharmaceutical formulations, providing an overall idea of the folding, self-assembly, and aggregation processes, which is challenging to obtain with high-resolution methods. Here, we compiled and discussed selected examples, together with our results and those that helped us better to understand the process of protein and peptide aggregation. We put particular emphasis on the basic description of the methods as well as on the experimental considerations needed to obtain meaningful information, to help those who are just getting into this exciting area of research. Moreover, this review is particularly useful to those out of the field who would like to improve reproducibility in their cellular and biomedical experiments, especially while working with peptide and protein systems as an external stimulus. Our final aim is to show the power of these low-resolution techniques to improve our understanding of the self-assembly of peptides and proteins and translate this fundamental knowledge in biomedical research or food applications.
format Online
Article
Text
id pubmed-7587993
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75879932020-10-29 Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods Pignataro, María Florencia Herrera, María Georgina Dodero, Verónica Isabel Molecules Review The self-assembly of proteins is an essential process for a variety of cellular functions including cell respiration, mobility and division. On the other hand, protein or peptide misfolding and aggregation is related to the development of Parkinson’s disease and Alzheimer’s disease, among other aggregopathies. As a consequence, significant research efforts are directed towards the understanding of this process. In this review, we are focused on the use of UV-Visible Absorption Spectroscopy, Fluorescence Spectroscopy and Circular Dichroism to evaluate the self-organization of proteins and peptides in solution. These spectroscopic techniques are commonly available in most chemistry and biochemistry research laboratories, and together they are a powerful approach for initial as well as routine evaluation of protein and peptide self-assembly and aggregation under different environmental stimulus. Furthermore, these spectroscopic techniques are even suitable for studying complex systems like those in the food industry or pharmaceutical formulations, providing an overall idea of the folding, self-assembly, and aggregation processes, which is challenging to obtain with high-resolution methods. Here, we compiled and discussed selected examples, together with our results and those that helped us better to understand the process of protein and peptide aggregation. We put particular emphasis on the basic description of the methods as well as on the experimental considerations needed to obtain meaningful information, to help those who are just getting into this exciting area of research. Moreover, this review is particularly useful to those out of the field who would like to improve reproducibility in their cellular and biomedical experiments, especially while working with peptide and protein systems as an external stimulus. Our final aim is to show the power of these low-resolution techniques to improve our understanding of the self-assembly of peptides and proteins and translate this fundamental knowledge in biomedical research or food applications. MDPI 2020-10-21 /pmc/articles/PMC7587993/ /pubmed/33096797 http://dx.doi.org/10.3390/molecules25204854 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pignataro, María Florencia
Herrera, María Georgina
Dodero, Verónica Isabel
Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods
title Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods
title_full Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods
title_fullStr Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods
title_full_unstemmed Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods
title_short Evaluation of Peptide/Protein Self-Assembly and Aggregation by Spectroscopic Methods
title_sort evaluation of peptide/protein self-assembly and aggregation by spectroscopic methods
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7587993/
https://www.ncbi.nlm.nih.gov/pubmed/33096797
http://dx.doi.org/10.3390/molecules25204854
work_keys_str_mv AT pignataromariaflorencia evaluationofpeptideproteinselfassemblyandaggregationbyspectroscopicmethods
AT herreramariageorgina evaluationofpeptideproteinselfassemblyandaggregationbyspectroscopicmethods
AT doderoveronicaisabel evaluationofpeptideproteinselfassemblyandaggregationbyspectroscopicmethods