Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species

Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes...

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Autores principales: Vetukuri, Ramesh R., Kalyandurg, Pruthvi B., Saripella, Ganapathi Varma, Sen, Diya, Gil, Jose Fernando, Lukhovitskaya, Nina I., Grenville-Briggs, Laura J., Savenkov, Eugene I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588395/
https://www.ncbi.nlm.nih.gov/pubmed/33040310
http://dx.doi.org/10.1007/s00705-020-04832-y
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author Vetukuri, Ramesh R.
Kalyandurg, Pruthvi B.
Saripella, Ganapathi Varma
Sen, Diya
Gil, Jose Fernando
Lukhovitskaya, Nina I.
Grenville-Briggs, Laura J.
Savenkov, Eugene I.
author_facet Vetukuri, Ramesh R.
Kalyandurg, Pruthvi B.
Saripella, Ganapathi Varma
Sen, Diya
Gil, Jose Fernando
Lukhovitskaya, Nina I.
Grenville-Briggs, Laura J.
Savenkov, Eugene I.
author_sort Vetukuri, Ramesh R.
collection PubMed
description Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5′-terminus, and changes in the expression of certain miRNAs, most of which were downregulated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-020-04832-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-75883952020-10-29 Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species Vetukuri, Ramesh R. Kalyandurg, Pruthvi B. Saripella, Ganapathi Varma Sen, Diya Gil, Jose Fernando Lukhovitskaya, Nina I. Grenville-Briggs, Laura J. Savenkov, Eugene I. Arch Virol Brief Report Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5′-terminus, and changes in the expression of certain miRNAs, most of which were downregulated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-020-04832-y) contains supplementary material, which is available to authorized users. Springer Vienna 2020-10-10 2020 /pmc/articles/PMC7588395/ /pubmed/33040310 http://dx.doi.org/10.1007/s00705-020-04832-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Report
Vetukuri, Ramesh R.
Kalyandurg, Pruthvi B.
Saripella, Ganapathi Varma
Sen, Diya
Gil, Jose Fernando
Lukhovitskaya, Nina I.
Grenville-Briggs, Laura J.
Savenkov, Eugene I.
Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species
title Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species
title_full Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species
title_fullStr Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species
title_full_unstemmed Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species
title_short Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species
title_sort effect of rna silencing suppression activity of chrysanthemum virus b p12 protein on small rna species
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588395/
https://www.ncbi.nlm.nih.gov/pubmed/33040310
http://dx.doi.org/10.1007/s00705-020-04832-y
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