Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories

The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Franci...

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Detalles Bibliográficos
Autores principales: Kesterson, Alexandria E., Craig, John E., Chuvala, Lara J., Heine, Henry S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588557/
https://www.ncbi.nlm.nih.gov/pubmed/33079040
http://dx.doi.org/10.3201/eid2611.191630
Descripción
Sumario:The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories.

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