Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories

The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Franci...

Descripción completa

Detalles Bibliográficos
Autores principales: Kesterson, Alexandria E., Craig, John E., Chuvala, Lara J., Heine, Henry S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2020
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588557/
https://www.ncbi.nlm.nih.gov/pubmed/33079040
http://dx.doi.org/10.3201/eid2611.191630
_version_ 1783600396473729024
author Kesterson, Alexandria E.
Craig, John E.
Chuvala, Lara J.
Heine, Henry S.
author_facet Kesterson, Alexandria E.
Craig, John E.
Chuvala, Lara J.
Heine, Henry S.
author_sort Kesterson, Alexandria E.
collection PubMed
description The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories.
format Online
Article
Text
id pubmed-7588557
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-75885572020-11-01 Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories Kesterson, Alexandria E. Craig, John E. Chuvala, Lara J. Heine, Henry S. Emerg Infect Dis Research The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories. Centers for Disease Control and Prevention 2020-11 /pmc/articles/PMC7588557/ /pubmed/33079040 http://dx.doi.org/10.3201/eid2611.191630 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Kesterson, Alexandria E.
Craig, John E.
Chuvala, Lara J.
Heine, Henry S.
Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
title Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
title_full Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
title_fullStr Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
title_full_unstemmed Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
title_short Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
title_sort validated methods for removing select agent samples from biosafety level 3 laboratories
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588557/
https://www.ncbi.nlm.nih.gov/pubmed/33079040
http://dx.doi.org/10.3201/eid2611.191630
work_keys_str_mv AT kestersonalexandriae validatedmethodsforremovingselectagentsamplesfrombiosafetylevel3laboratories
AT craigjohne validatedmethodsforremovingselectagentsamplesfrombiosafetylevel3laboratories
AT chuvalalaraj validatedmethodsforremovingselectagentsamplesfrombiosafetylevel3laboratories
AT heinehenrys validatedmethodsforremovingselectagentsamplesfrombiosafetylevel3laboratories

Ejemplares similares