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Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus
A marine-derived actinomycete (Streptomyces sp. MBTI36) exhibiting antibacterial activities was investigated in the present study. The strain was identified using genetic techniques. The 16S rDNA sequence of the isolate indicated that it was most closely related to Streptomyces microflavus. Furtherm...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588889/ https://www.ncbi.nlm.nih.gov/pubmed/33096696 http://dx.doi.org/10.3390/md18100522 |
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author | Cho, Eunji Kwon, Oh-Seok Chung, Beomkoo Lee, Jayho Sun, Jeongyoon Shin, Jongheon Oh, Ki-Bong |
author_facet | Cho, Eunji Kwon, Oh-Seok Chung, Beomkoo Lee, Jayho Sun, Jeongyoon Shin, Jongheon Oh, Ki-Bong |
author_sort | Cho, Eunji |
collection | PubMed |
description | A marine-derived actinomycete (Streptomyces sp. MBTI36) exhibiting antibacterial activities was investigated in the present study. The strain was identified using genetic techniques. The 16S rDNA sequence of the isolate indicated that it was most closely related to Streptomyces microflavus. Furthermore, a new chromomycin A(9) (1), along with chromomycin Ap (2), chromomycin A(2) (3), and chromomycin A(3) (4), were isolated from the ethyl acetate extract. Their structures were determined using extensive spectroscopic methods including 1D and 2D NMR, and HRMS, as well as comparisons with previously reported data. Compounds 1–4 showed potent antibacterial activities against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). During a passage experiment, minimum inhibitory concentration (MIC) values for compounds 1–4 showed no more than a 4-fold increase from the starting MIC value, indicating that no resistance was detected over the 21 passages. |
format | Online Article Text |
id | pubmed-7588889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75888892020-10-29 Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus Cho, Eunji Kwon, Oh-Seok Chung, Beomkoo Lee, Jayho Sun, Jeongyoon Shin, Jongheon Oh, Ki-Bong Mar Drugs Article A marine-derived actinomycete (Streptomyces sp. MBTI36) exhibiting antibacterial activities was investigated in the present study. The strain was identified using genetic techniques. The 16S rDNA sequence of the isolate indicated that it was most closely related to Streptomyces microflavus. Furthermore, a new chromomycin A(9) (1), along with chromomycin Ap (2), chromomycin A(2) (3), and chromomycin A(3) (4), were isolated from the ethyl acetate extract. Their structures were determined using extensive spectroscopic methods including 1D and 2D NMR, and HRMS, as well as comparisons with previously reported data. Compounds 1–4 showed potent antibacterial activities against Gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA). During a passage experiment, minimum inhibitory concentration (MIC) values for compounds 1–4 showed no more than a 4-fold increase from the starting MIC value, indicating that no resistance was detected over the 21 passages. MDPI 2020-10-21 /pmc/articles/PMC7588889/ /pubmed/33096696 http://dx.doi.org/10.3390/md18100522 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cho, Eunji Kwon, Oh-Seok Chung, Beomkoo Lee, Jayho Sun, Jeongyoon Shin, Jongheon Oh, Ki-Bong Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus |
title | Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus |
title_full | Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus |
title_fullStr | Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus |
title_full_unstemmed | Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus |
title_short | Antibacterial Activity of Chromomycins from a Marine-Derived Streptomyces microflavus |
title_sort | antibacterial activity of chromomycins from a marine-derived streptomyces microflavus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588889/ https://www.ncbi.nlm.nih.gov/pubmed/33096696 http://dx.doi.org/10.3390/md18100522 |
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