Cargando…

A Novel HPLC-Based Method to Investigate on RNA after Fixation

RNA isolated from fixed and paraffin-embedded tissues is widely used in biomedical research and molecular pathology for diagnosis. In the present study, we have set-up a method based on high performance liquid chromatography (HPLC) to investigate the effects of different fixatives on RNA. By the app...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fattorini, Paolo, Forzato, Cristina, Tierno, Domenico, De Martino, Eleonora, Azzalini, Eros, Canzonieri, Vincenzo, Stanta, Giorgio, Bonin, Serena
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588918/ https://www.ncbi.nlm.nih.gov/pubmed/33066070 http://dx.doi.org/10.3390/ijms21207540

Ejemplares similares

Reliability of miRNA Analysis from Fixed and Paraffin-Embedded Tissues
por: Azzalini, Eros, et al.
Publicado: (2019)

Overview of Tumor Heterogeneity in High-Grade Serous Ovarian Cancers
por: Azzalini, Eros, et al.
Publicado: (2023)

AKT Isoforms Interplay in High-Grade Serous Ovarian Cancer Prognosis and Characterization
por: Azzalini, Eros, et al.
Publicado: (2022)

Nanomechanical Characterization of Ovarian Cancer Cell Lines as a Marker of Response to 2c Treatment
por: Tierno, Domenico, et al.
Publicado: (2023)

Overview on Clinical Relevance of Intra-Tumor Heterogeneity
por: Stanta, Giorgio, et al.
Publicado: (2018)

Evaluation of a Set of miRNAs in 26 Cases of Fatal Traumatic Brain Injuries
por: Bonin, Serena, et al.
Publicado: (2023)

A group of three miRNAs can act as candidate circulating biomarkers in liquid biopsies from melanoma patients
por: De Martino, Eleonora, et al.
Publicado: (2023)

Management of stage II colon cancer - the use of molecular biomarkers for adjuvant therapy decision
por: Donada, Marisa, et al.
Publicado: (2013)

The Association of Residential Altitude on the Molecular Profile and Survival of Melanoma: Results of an Interreg Study
por: De Martino, Eleonora, et al.
Publicado: (2020)

Oleocanthal Quantification Using (1)H NMR Spectroscopy and Polyphenols HPLC Analysis of Olive Oil from the Bianchera/Belica Cultivar
por: Starec, Martina, et al.
Publicado: (2021)

Is Human Papillomavirus Associated with Prostate Cancer Survival?
por: Pascale, Mariarosa, et al.
Publicado: (2013)

Are Breast Cancer Molecular Classes Predictive of Survival in Patients with Long Follow-Up?
por: Pracella, Danae, et al.
Publicado: (2013)

Primary intestinal-type adenocarcinoma of the vulva. A case report and review of the literature
por: Sopracordevole, Francesco, et al.
Publicado: (2023)

Reduced expression of α-L-Fucosidase-1 (FUCA-1) predicts recurrence and shorter cancer specific survival in luminal B LN+ breast cancer patients
por: Bonin, Serena, et al.
Publicado: (2018)

Mycosis fungoides: is it a Borrelia burgdorferi-associated disease?
por: Tothova, S Miertusova, et al.
Publicado: (2006)

The proliferation marker Ki67, but not neuroendocrine expression, is an independent factor in the prediction of prognosis of primary prostate cancer patients
por: Pascale, Mariarosa, et al.
Publicado: (2016)

PI3K/AKT Signaling in Breast Cancer Molecular Subtyping and Lymph Node Involvement
por: Bonin, S., et al.
Publicado: (2019)

New Diterpenes with Potential Antitumoral Activity Isolated from Plants in the Years 2017–2022
por: Forzato, Cristina, et al.
Publicado: (2022)

Fixation and staining methods for macroscopical investigation of the brain
por: Nardi, Leonardo, et al.
Publicado: (2023)

The human RECQ1 helicase is highly expressed in glioblastoma and plays an important role in tumor cell proliferation
por: Mendoza-Maldonado, Ramiro, et al.
Publicado: (2011)

Practical HPLC method development /
por: Snyder, Lloyd R.
Publicado: (1988)

Analysis of Chlorophylls/Chlorophyllins in Food Products Using HPLC and HPLC-MS Methods
por: Mandal, Badal Kumar, et al.
Publicado: (2023)

Biosensors and Sensing Systems for Rapid Analysis of Phenolic Compounds from Plants: A Comprehensive Review
por: Forzato, Cristina, et al.
Publicado: (2020)

Prolonged DNA hydrolysis in water: A study on DNA stability
por: Fattorini, Paolo, et al.
Publicado: (2018)

The Baron Pasquale Revoltella’s Will in the Forensic Genetics Era
por: Fattorini, Paolo, et al.
Publicado: (2023)

Development of Micellar HPLC-UV Method for Determination of Pharmaceuticals in Water Samples
por: da Silva, Danielle Cristina, et al.
Publicado: (2018)

A Novel HPLC-Assisted Method for Investigation of the Fe(2+)-Chelating Activity of Flavonoids and Plant Extracts
por: Olennikov, Daniil N., et al.
Publicado: (2014)

A Validated HPLC-PDA-HRMS Method to Investigate the Biological Stability and Metabolism of Antiparasitic Triterpenic Esters
por: Schioppa, Laura, et al.
Publicado: (2021)

Investigation of the transformations of a novel anti-cancer agent combining HPLC, HPLC–MS and direct ESI–HRMS analyses
por: Tietze, Lutz F., et al.
Publicado: (2009)

Hydroxycinnamoyl Amino Acids Conjugates: A Chiral Pool to Distinguish Commercially Exploited Coffea spp.
por: Berti, Federico, et al.
Publicado: (2020)

Validation of the HPLC Analytical Method for the Determination of Chemical and Radiochemical Purity of Ga-68-DOTATATE
por: Sammartano, Antonino, et al.
Publicado: (2023)

Microwave-Assisted Extraction and HPLC-UV-CD Determination of (S)-usnic Acid in Cladonia foliacea
por: Cavalloro, Valeria, et al.
Publicado: (2021)

Amanitins in Wild Mushrooms: The Development of HPLC-UV-EC and HPLC-DAD-MS Methods for Food Safety Purposes
por: Barbosa, Isabel, et al.
Publicado: (2022)

Lamalbid, Chlorogenic Acid, and Verbascoside as Tools for Standardization of Lamium album Flowers—Development and Validation of HPLC–DAD Method
por: Czerwińska, Monika E., et al.
Publicado: (2020)

Development and Validation of HPLC Method for the Determination of Pregabalin in Capsules
por: Kasawar, G. B., et al.
Publicado: (2010)

Development of Spectrofluorimetric and HPLC Methods for In vitro Analysis of Repaglinide
por: Kaushal, N., et al.
Publicado: (2010)

Stability-Indicating HPLC Method for Posaconazole Bulk Assay
por: Garcia, Cássia V., et al.
Publicado: (2012)

Stability-Indicating HPLC Method for the Determination of Cefcapene Pivoxil
por: Zalewski, Przemysław, et al.
Publicado: (2012)

Validation of Quantitative HPLC Method for Bacosides in KeenMind
por: Dowell, Ashley, et al.
Publicado: (2015)

A Specific HPLC Method to Determine Residual HEPES in [(68)Ga]Ga-Radiopharmaceuticals: Development and Validation
por: Migliari, Silvia, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_rc2epi50kr25ovstp87be6ba2u