Cargando…
Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass
γ-Aminobutyric acid (GABA) plays an important role in regulating stress tolerance in plants. Purposes of this study was to determine the effect of an exogenous supply of GABA on tolerance to water stress in creeping bentgrass (Agrostis stolonifera), and further reveal the GABA-induced key mechanisms...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589152/ https://www.ncbi.nlm.nih.gov/pubmed/33050389 http://dx.doi.org/10.3390/ijms21207460 |
_version_ | 1783600512847839232 |
---|---|
author | Tang, Mingyan Li, Zhou Luo, Ling Cheng, Bizhen Zhang, Youzhi Zeng, Weihang Peng, Yan |
author_facet | Tang, Mingyan Li, Zhou Luo, Ling Cheng, Bizhen Zhang, Youzhi Zeng, Weihang Peng, Yan |
author_sort | Tang, Mingyan |
collection | PubMed |
description | γ-Aminobutyric acid (GABA) plays an important role in regulating stress tolerance in plants. Purposes of this study was to determine the effect of an exogenous supply of GABA on tolerance to water stress in creeping bentgrass (Agrostis stolonifera), and further reveal the GABA-induced key mechanisms related to water balance, nitrogen (N) metabolism and nitric oxide (NO) production in response to water stress. Plants were pretreated with or without 0.5 mM GABA solution in the roots for 3 days, and then subjected to water stress induced by −0.52 MPa polyethylene glycol 6000 for 12 days. The results showed that water stress caused leaf water deficit, chlorophyll (Chl) loss, oxidative damage (increases in superoxide anion, hydrogen peroxide, malondialdehyde, and protein carbonyl content), N insufficiency, and metabolic disturbance. However, the exogenous addition of GABA significantly increased endogenous GABA content, osmotic adjustment and antioxidant enzyme activities (superoxide dismutase, catalase, dehydroascorbate reductase, glutathione reductase and monodehydroascorbate reductase), followed by effectively alleviating water stress damage, including declines in oxidative damage, photoinhibition, and water and Chl loss. GABA supply not only provided more available N, but also affected N metabolism through activating nitrite reductase and glutamine synthetase activities under water stress. The supply of GABA did not increase glutamate content and glutamate decarboxylase activity, but enhanced glutamate dehydrogenase activity, which might indicate that GABA promoted the conversion and utilization of glutamate for maintaining Chl synthesis and tricarboxylic acid cycle when creeping bentgrass underwent water stress. In addition, GABA-induced NO production, depending on nitrate reductase and NO-associated protein pathways, could be associated with the enhancement of antioxidant defense. Current findings reveal the critical role of GABA in regulating signal transduction and metabolic homeostasis in plants under water-limited condition. |
format | Online Article Text |
id | pubmed-7589152 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75891522020-10-29 Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass Tang, Mingyan Li, Zhou Luo, Ling Cheng, Bizhen Zhang, Youzhi Zeng, Weihang Peng, Yan Int J Mol Sci Article γ-Aminobutyric acid (GABA) plays an important role in regulating stress tolerance in plants. Purposes of this study was to determine the effect of an exogenous supply of GABA on tolerance to water stress in creeping bentgrass (Agrostis stolonifera), and further reveal the GABA-induced key mechanisms related to water balance, nitrogen (N) metabolism and nitric oxide (NO) production in response to water stress. Plants were pretreated with or without 0.5 mM GABA solution in the roots for 3 days, and then subjected to water stress induced by −0.52 MPa polyethylene glycol 6000 for 12 days. The results showed that water stress caused leaf water deficit, chlorophyll (Chl) loss, oxidative damage (increases in superoxide anion, hydrogen peroxide, malondialdehyde, and protein carbonyl content), N insufficiency, and metabolic disturbance. However, the exogenous addition of GABA significantly increased endogenous GABA content, osmotic adjustment and antioxidant enzyme activities (superoxide dismutase, catalase, dehydroascorbate reductase, glutathione reductase and monodehydroascorbate reductase), followed by effectively alleviating water stress damage, including declines in oxidative damage, photoinhibition, and water and Chl loss. GABA supply not only provided more available N, but also affected N metabolism through activating nitrite reductase and glutamine synthetase activities under water stress. The supply of GABA did not increase glutamate content and glutamate decarboxylase activity, but enhanced glutamate dehydrogenase activity, which might indicate that GABA promoted the conversion and utilization of glutamate for maintaining Chl synthesis and tricarboxylic acid cycle when creeping bentgrass underwent water stress. In addition, GABA-induced NO production, depending on nitrate reductase and NO-associated protein pathways, could be associated with the enhancement of antioxidant defense. Current findings reveal the critical role of GABA in regulating signal transduction and metabolic homeostasis in plants under water-limited condition. MDPI 2020-10-10 /pmc/articles/PMC7589152/ /pubmed/33050389 http://dx.doi.org/10.3390/ijms21207460 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tang, Mingyan Li, Zhou Luo, Ling Cheng, Bizhen Zhang, Youzhi Zeng, Weihang Peng, Yan Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass |
title | Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass |
title_full | Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass |
title_fullStr | Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass |
title_full_unstemmed | Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass |
title_short | Nitric Oxide Signal, Nitrogen Metabolism, and Water Balance Affected by γ-Aminobutyric Acid (GABA) in Relation to Enhanced Tolerance to Water Stress in Creeping Bentgrass |
title_sort | nitric oxide signal, nitrogen metabolism, and water balance affected by γ-aminobutyric acid (gaba) in relation to enhanced tolerance to water stress in creeping bentgrass |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589152/ https://www.ncbi.nlm.nih.gov/pubmed/33050389 http://dx.doi.org/10.3390/ijms21207460 |
work_keys_str_mv | AT tangmingyan nitricoxidesignalnitrogenmetabolismandwaterbalanceaffectedbygaminobutyricacidgabainrelationtoenhancedtolerancetowaterstressincreepingbentgrass AT lizhou nitricoxidesignalnitrogenmetabolismandwaterbalanceaffectedbygaminobutyricacidgabainrelationtoenhancedtolerancetowaterstressincreepingbentgrass AT luoling nitricoxidesignalnitrogenmetabolismandwaterbalanceaffectedbygaminobutyricacidgabainrelationtoenhancedtolerancetowaterstressincreepingbentgrass AT chengbizhen nitricoxidesignalnitrogenmetabolismandwaterbalanceaffectedbygaminobutyricacidgabainrelationtoenhancedtolerancetowaterstressincreepingbentgrass AT zhangyouzhi nitricoxidesignalnitrogenmetabolismandwaterbalanceaffectedbygaminobutyricacidgabainrelationtoenhancedtolerancetowaterstressincreepingbentgrass AT zengweihang nitricoxidesignalnitrogenmetabolismandwaterbalanceaffectedbygaminobutyricacidgabainrelationtoenhancedtolerancetowaterstressincreepingbentgrass AT pengyan nitricoxidesignalnitrogenmetabolismandwaterbalanceaffectedbygaminobutyricacidgabainrelationtoenhancedtolerancetowaterstressincreepingbentgrass |