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Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy
The biological function of high‐density lipoprotein (HDL) nanoparticles, the so‐called good cholesterol that is associated with a low risk of heart disease, depends on their composition, morphology, and size. The morphology of HDL particles composed of apolipoproteins, lipids and cholesterol is rout...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589421/ https://www.ncbi.nlm.nih.gov/pubmed/32542937 http://dx.doi.org/10.1002/anie.202004130 |
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author | Lau, Sophie Middleton, David A. |
author_facet | Lau, Sophie Middleton, David A. |
author_sort | Lau, Sophie |
collection | PubMed |
description | The biological function of high‐density lipoprotein (HDL) nanoparticles, the so‐called good cholesterol that is associated with a low risk of heart disease, depends on their composition, morphology, and size. The morphology of HDL particles composed of apolipoproteins, lipids and cholesterol is routinely visualised by transmission electron microscopy (TEM), but higher‐resolution tools are needed to observe more subtle structural differences between particles of different composition. Here, reconstituted HDL formulations are oriented on glass substrates and solid‐state (31)P NMR spectroscopy is shown to be highly sensitive to the surface curvature of the lipid headgroups. The spectra report potentially functionally important differences in the morphology of different HDL preparations that are not detected by TEM. This method provides new morphological insights into HDL comprising a naturally occurring apolipoprotein A‐I mutant, which may be linked to its atheroprotective properties, and holds promise as a future research tool in the clinical analysis of plasma HDL. |
format | Online Article Text |
id | pubmed-7589421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75894212020-10-30 Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy Lau, Sophie Middleton, David A. Angew Chem Int Ed Engl Communications The biological function of high‐density lipoprotein (HDL) nanoparticles, the so‐called good cholesterol that is associated with a low risk of heart disease, depends on their composition, morphology, and size. The morphology of HDL particles composed of apolipoproteins, lipids and cholesterol is routinely visualised by transmission electron microscopy (TEM), but higher‐resolution tools are needed to observe more subtle structural differences between particles of different composition. Here, reconstituted HDL formulations are oriented on glass substrates and solid‐state (31)P NMR spectroscopy is shown to be highly sensitive to the surface curvature of the lipid headgroups. The spectra report potentially functionally important differences in the morphology of different HDL preparations that are not detected by TEM. This method provides new morphological insights into HDL comprising a naturally occurring apolipoprotein A‐I mutant, which may be linked to its atheroprotective properties, and holds promise as a future research tool in the clinical analysis of plasma HDL. John Wiley and Sons Inc. 2020-08-17 2020-10-05 /pmc/articles/PMC7589421/ /pubmed/32542937 http://dx.doi.org/10.1002/anie.202004130 Text en © 2020 The Authors. Published by Wiley-VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Communications Lau, Sophie Middleton, David A. Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy |
title | Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy |
title_full | Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy |
title_fullStr | Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy |
title_full_unstemmed | Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy |
title_short | Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy |
title_sort | sensitive morphological characterization of oriented high‐density lipoprotein nanoparticles using (31)p nmr spectroscopy |
topic | Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589421/ https://www.ncbi.nlm.nih.gov/pubmed/32542937 http://dx.doi.org/10.1002/anie.202004130 |
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