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Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy

The biological function of high‐density lipoprotein (HDL) nanoparticles, the so‐called good cholesterol that is associated with a low risk of heart disease, depends on their composition, morphology, and size. The morphology of HDL particles composed of apolipoproteins, lipids and cholesterol is rout...

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Autores principales: Lau, Sophie, Middleton, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589421/
https://www.ncbi.nlm.nih.gov/pubmed/32542937
http://dx.doi.org/10.1002/anie.202004130
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author Lau, Sophie
Middleton, David A.
author_facet Lau, Sophie
Middleton, David A.
author_sort Lau, Sophie
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description The biological function of high‐density lipoprotein (HDL) nanoparticles, the so‐called good cholesterol that is associated with a low risk of heart disease, depends on their composition, morphology, and size. The morphology of HDL particles composed of apolipoproteins, lipids and cholesterol is routinely visualised by transmission electron microscopy (TEM), but higher‐resolution tools are needed to observe more subtle structural differences between particles of different composition. Here, reconstituted HDL formulations are oriented on glass substrates and solid‐state (31)P NMR spectroscopy is shown to be highly sensitive to the surface curvature of the lipid headgroups. The spectra report potentially functionally important differences in the morphology of different HDL preparations that are not detected by TEM. This method provides new morphological insights into HDL comprising a naturally occurring apolipoprotein A‐I mutant, which may be linked to its atheroprotective properties, and holds promise as a future research tool in the clinical analysis of plasma HDL.
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spelling pubmed-75894212020-10-30 Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy Lau, Sophie Middleton, David A. Angew Chem Int Ed Engl Communications The biological function of high‐density lipoprotein (HDL) nanoparticles, the so‐called good cholesterol that is associated with a low risk of heart disease, depends on their composition, morphology, and size. The morphology of HDL particles composed of apolipoproteins, lipids and cholesterol is routinely visualised by transmission electron microscopy (TEM), but higher‐resolution tools are needed to observe more subtle structural differences between particles of different composition. Here, reconstituted HDL formulations are oriented on glass substrates and solid‐state (31)P NMR spectroscopy is shown to be highly sensitive to the surface curvature of the lipid headgroups. The spectra report potentially functionally important differences in the morphology of different HDL preparations that are not detected by TEM. This method provides new morphological insights into HDL comprising a naturally occurring apolipoprotein A‐I mutant, which may be linked to its atheroprotective properties, and holds promise as a future research tool in the clinical analysis of plasma HDL. John Wiley and Sons Inc. 2020-08-17 2020-10-05 /pmc/articles/PMC7589421/ /pubmed/32542937 http://dx.doi.org/10.1002/anie.202004130 Text en © 2020 The Authors. Published by Wiley-VCH GmbH This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Lau, Sophie
Middleton, David A.
Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy
title Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy
title_full Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy
title_fullStr Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy
title_full_unstemmed Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy
title_short Sensitive Morphological Characterization of Oriented High‐Density Lipoprotein Nanoparticles Using (31)P NMR Spectroscopy
title_sort sensitive morphological characterization of oriented high‐density lipoprotein nanoparticles using (31)p nmr spectroscopy
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589421/
https://www.ncbi.nlm.nih.gov/pubmed/32542937
http://dx.doi.org/10.1002/anie.202004130
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