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Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recomb...

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Autores principales: Pedersen, Tobias Bruun, Nielsen, Mikkel Rank, Kristensen, Sebastian Birkedal, Spedtsberg, Eva Mie Lang, Yasmine, Wafaa, Matthiesen, Rikke, Kaniki, Samba Evelyne Kabemba, Sørensen, Trine, Petersen, Celine, Muff, Jens, Sondergaard, Teis Esben, Nielsen, Kåre Lehmann, Wimmer, Reinhard, Sørensen, Jens Laurids
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589453/
https://www.ncbi.nlm.nih.gov/pubmed/33066643
http://dx.doi.org/10.3390/ijms21207601
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author Pedersen, Tobias Bruun
Nielsen, Mikkel Rank
Kristensen, Sebastian Birkedal
Spedtsberg, Eva Mie Lang
Yasmine, Wafaa
Matthiesen, Rikke
Kaniki, Samba Evelyne Kabemba
Sørensen, Trine
Petersen, Celine
Muff, Jens
Sondergaard, Teis Esben
Nielsen, Kåre Lehmann
Wimmer, Reinhard
Sørensen, Jens Laurids
author_facet Pedersen, Tobias Bruun
Nielsen, Mikkel Rank
Kristensen, Sebastian Birkedal
Spedtsberg, Eva Mie Lang
Yasmine, Wafaa
Matthiesen, Rikke
Kaniki, Samba Evelyne Kabemba
Sørensen, Trine
Petersen, Celine
Muff, Jens
Sondergaard, Teis Esben
Nielsen, Kåre Lehmann
Wimmer, Reinhard
Sørensen, Jens Laurids
author_sort Pedersen, Tobias Bruun
collection PubMed
description Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1–4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.
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spelling pubmed-75894532020-10-29 Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani Pedersen, Tobias Bruun Nielsen, Mikkel Rank Kristensen, Sebastian Birkedal Spedtsberg, Eva Mie Lang Yasmine, Wafaa Matthiesen, Rikke Kaniki, Samba Evelyne Kabemba Sørensen, Trine Petersen, Celine Muff, Jens Sondergaard, Teis Esben Nielsen, Kåre Lehmann Wimmer, Reinhard Sørensen, Jens Laurids Int J Mol Sci Communication Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1–4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L. MDPI 2020-10-14 /pmc/articles/PMC7589453/ /pubmed/33066643 http://dx.doi.org/10.3390/ijms21207601 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Pedersen, Tobias Bruun
Nielsen, Mikkel Rank
Kristensen, Sebastian Birkedal
Spedtsberg, Eva Mie Lang
Yasmine, Wafaa
Matthiesen, Rikke
Kaniki, Samba Evelyne Kabemba
Sørensen, Trine
Petersen, Celine
Muff, Jens
Sondergaard, Teis Esben
Nielsen, Kåre Lehmann
Wimmer, Reinhard
Sørensen, Jens Laurids
Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani
title Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani
title_full Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani
title_fullStr Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani
title_full_unstemmed Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani
title_short Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani
title_sort heterologous expression of the core genes in the complex fusarubin gene cluster of fusarium solani
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589453/
https://www.ncbi.nlm.nih.gov/pubmed/33066643
http://dx.doi.org/10.3390/ijms21207601
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