High Exposure to Toxoplasma gondii and Neospora Spp. in Donkeys in Israel: Serological Survey and Case Reports

SIMPLE SUMMARY: Toxoplasma gondii and Neospora spp. are major pathogenic parasites of animals worldwide, with the first also affecting humans. These parasites have two-host life cycles, with the cat and the dog being the definitive hosts of T. gondii and N. caninum, respectively. Both parasites can infect various animal species, as intermediate hosts, in which they form tissue cysts and may cause abortions and neurological disease. Both parasites have been reported in wild and domestic animals in Israel. This study aimed to evaluate the serologic exposure of donkeys to these parasites. A total of 98 donkeys were examined. Half of them (n = 49) were from animal shelters in Israel, and the rest (n = 49) were working donkeys from the Palestinian Authority. Anti-T. gondii antibodies and anti-Neospora spp. antibodies were found in 94% and in 70% of the donkeys, respectively. In addition, two cases of donkeys presenting N. caninum tissue cysts, which were detected during post-mortem examination, were described. This is the first report of the exposure of donkeys to Toxoplasma gondii and Neospora spp. in the area. The exposure of donkeys to both parasites was considerably higher than the exposure of other species in the area and may be the result of poor husbandry conditions and higher exposure to infection. These results indicate that donkeys may have an important role in the maintenance and transmission of these parasites. ABSTRACT: Toxoplasma gondii and Neospora spp. are closely related cyst-forming coccidian parasites, which infect various animal species and have considerable zoonotic and economic implications, respectively. Both parasites are endemic in Israel and have been reported to infect wild and domestic animals. This study was conceived to evaluate the serologic exposure of donkeys to these parasites. Serum samples were collected from 98 donkeys. Half of them (n = 49) were from animal shelters in Israel, and the rest (n = 49) were working donkeys from the Palestinian Authority. The donkeys were screened for the presence of anti-Toxoplasma and anti-Neospora antibodies by immunofluorescence antibody tests (IFATs). The seroprevalence of T. gondii and Neospora spp. was 94% and 70%, respectively, and 69% of the donkeys were exposed to both parasites. In addition, N. caninum tissue cysts were documented in two donkeys during post-mortem examination. This is the first report of the exposure of donkeys to T. gondii and Neospora spp. in the area. The high prevalence found in this study suggests that donkeys may have a role in the maintenance of these parasites in the area, thus serving as a source of infection for the definitive hosts.