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GAGA Regulates Border Cell Migration in Drosophila

Collective cell migration is a complex process that happens during normal development of many multicellular organisms, as well as during oncological transformations. In Drosophila oogenesis, a small set of follicle cells originally located at the anterior tip of each egg chamber become motile and mi...

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Autores principales: Ogienko, Anna A., Yarinich, Lyubov A., Fedorova, Elena V., Dorogova, Natalya V., Bayborodin, Sergey I., Baricheva, Elina M., Pindyurin, Alexey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589894/
https://www.ncbi.nlm.nih.gov/pubmed/33050455
http://dx.doi.org/10.3390/ijms21207468
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author Ogienko, Anna A.
Yarinich, Lyubov A.
Fedorova, Elena V.
Dorogova, Natalya V.
Bayborodin, Sergey I.
Baricheva, Elina M.
Pindyurin, Alexey V.
author_facet Ogienko, Anna A.
Yarinich, Lyubov A.
Fedorova, Elena V.
Dorogova, Natalya V.
Bayborodin, Sergey I.
Baricheva, Elina M.
Pindyurin, Alexey V.
author_sort Ogienko, Anna A.
collection PubMed
description Collective cell migration is a complex process that happens during normal development of many multicellular organisms, as well as during oncological transformations. In Drosophila oogenesis, a small set of follicle cells originally located at the anterior tip of each egg chamber become motile and migrate as a cluster through nurse cells toward the oocyte. These specialized cells are referred to as border cells (BCs) and provide a simple and convenient model system to study collective cell migration. The process is known to be complexly regulated at different levels and the product of the slow border cells (slbo) gene, the C/EBP transcription factor, is one of the key elements in this process. However, little is known about the regulation of slbo expression. On the other hand, the ubiquitously expressed transcription factor GAGA, which is encoded by the Trithorax-like (Trl) gene was previously demonstrated to be important for Drosophila oogenesis. Here, we found that Trl mutations cause substantial defects in BC migration. Partially, these defects are explained by the reduced level of slbo expression in BCs. Additionally, a strong genetic interaction between Trl and slbo mutants, along with the presence of putative GAGA binding sites within the slbo promoter and enhancer, suggests the direct regulation of this gene by GAGA. This idea is supported by the reduction in the slbo-Gal4-driven GFP expression within BC clusters in Trl mutant background. However, the inability of slbo overexpression to compensate defects in BC migration caused by Trl mutations suggests that there are other GAGA target genes contributing to this process. Taken together, the results define GAGA as another important regulator of BC migration in Drosophila oogenesis.
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spelling pubmed-75898942020-10-29 GAGA Regulates Border Cell Migration in Drosophila Ogienko, Anna A. Yarinich, Lyubov A. Fedorova, Elena V. Dorogova, Natalya V. Bayborodin, Sergey I. Baricheva, Elina M. Pindyurin, Alexey V. Int J Mol Sci Article Collective cell migration is a complex process that happens during normal development of many multicellular organisms, as well as during oncological transformations. In Drosophila oogenesis, a small set of follicle cells originally located at the anterior tip of each egg chamber become motile and migrate as a cluster through nurse cells toward the oocyte. These specialized cells are referred to as border cells (BCs) and provide a simple and convenient model system to study collective cell migration. The process is known to be complexly regulated at different levels and the product of the slow border cells (slbo) gene, the C/EBP transcription factor, is one of the key elements in this process. However, little is known about the regulation of slbo expression. On the other hand, the ubiquitously expressed transcription factor GAGA, which is encoded by the Trithorax-like (Trl) gene was previously demonstrated to be important for Drosophila oogenesis. Here, we found that Trl mutations cause substantial defects in BC migration. Partially, these defects are explained by the reduced level of slbo expression in BCs. Additionally, a strong genetic interaction between Trl and slbo mutants, along with the presence of putative GAGA binding sites within the slbo promoter and enhancer, suggests the direct regulation of this gene by GAGA. This idea is supported by the reduction in the slbo-Gal4-driven GFP expression within BC clusters in Trl mutant background. However, the inability of slbo overexpression to compensate defects in BC migration caused by Trl mutations suggests that there are other GAGA target genes contributing to this process. Taken together, the results define GAGA as another important regulator of BC migration in Drosophila oogenesis. MDPI 2020-10-10 /pmc/articles/PMC7589894/ /pubmed/33050455 http://dx.doi.org/10.3390/ijms21207468 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ogienko, Anna A.
Yarinich, Lyubov A.
Fedorova, Elena V.
Dorogova, Natalya V.
Bayborodin, Sergey I.
Baricheva, Elina M.
Pindyurin, Alexey V.
GAGA Regulates Border Cell Migration in Drosophila
title GAGA Regulates Border Cell Migration in Drosophila
title_full GAGA Regulates Border Cell Migration in Drosophila
title_fullStr GAGA Regulates Border Cell Migration in Drosophila
title_full_unstemmed GAGA Regulates Border Cell Migration in Drosophila
title_short GAGA Regulates Border Cell Migration in Drosophila
title_sort gaga regulates border cell migration in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589894/
https://www.ncbi.nlm.nih.gov/pubmed/33050455
http://dx.doi.org/10.3390/ijms21207468
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