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Impact of Polyallylamine Hydrochloride on Gene Expression and Karyotypic Stability of Multidrug Resistant Transformed Cells

The synthetic polymer, polyallylamine hydrochloride (PAA), is found in a variety of applications in biotechnology and medicine. It is used in gene and siRNA transfer, to form microcapsules for targeted drug delivery to damaged and tumor cells. Conventional chemotherapy often does not kill all cancer...

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Autores principales: Alekseenko, Larisa, Shilina, Mariia, Kozhukharova, Irina, Lyublinskaya, Olga, Fridlyanskaya, Irina, Nikolsky, Nikolay, Grinchuk, Tatiana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589997/
https://www.ncbi.nlm.nih.gov/pubmed/33096691
http://dx.doi.org/10.3390/cells9102332
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author Alekseenko, Larisa
Shilina, Mariia
Kozhukharova, Irina
Lyublinskaya, Olga
Fridlyanskaya, Irina
Nikolsky, Nikolay
Grinchuk, Tatiana
author_facet Alekseenko, Larisa
Shilina, Mariia
Kozhukharova, Irina
Lyublinskaya, Olga
Fridlyanskaya, Irina
Nikolsky, Nikolay
Grinchuk, Tatiana
author_sort Alekseenko, Larisa
collection PubMed
description The synthetic polymer, polyallylamine hydrochloride (PAA), is found in a variety of applications in biotechnology and medicine. It is used in gene and siRNA transfer, to form microcapsules for targeted drug delivery to damaged and tumor cells. Conventional chemotherapy often does not kill all cancer cells and leads to multidrug resistance (MDR). Until recently, studies of the effects of PAA on cells have mainly focused on their morphological and genetic characteristics immediately or several hours after exposure to the polymer. The properties of the cell progeny which survived the sublethal effects of PAA and resumed their proliferation, were not monitored. The present study demonstrated that treatment of immortalized Chinese hamster cells CHLV-79 RJK sensitive (RJK) and resistant (RJKEB) to ethidium bromide (EB) with cytotoxic doses of PAA, selected cells with increased karyotypic instability, were accompanied by changes in the expression of p53 genes c-fos, topo2-α, hsp90, hsc70. These changes did not contribute to the progression of MDR, accompanied by the increased sensitivity of these cells to the toxic effects of doxorubicin (DOX). Our results showed that PAA does not increase the oncogenic potential of immortalized cells and confirmed that it can be used for intracellular drug delivery for anticancer therapy.
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spelling pubmed-75899972020-10-29 Impact of Polyallylamine Hydrochloride on Gene Expression and Karyotypic Stability of Multidrug Resistant Transformed Cells Alekseenko, Larisa Shilina, Mariia Kozhukharova, Irina Lyublinskaya, Olga Fridlyanskaya, Irina Nikolsky, Nikolay Grinchuk, Tatiana Cells Article The synthetic polymer, polyallylamine hydrochloride (PAA), is found in a variety of applications in biotechnology and medicine. It is used in gene and siRNA transfer, to form microcapsules for targeted drug delivery to damaged and tumor cells. Conventional chemotherapy often does not kill all cancer cells and leads to multidrug resistance (MDR). Until recently, studies of the effects of PAA on cells have mainly focused on their morphological and genetic characteristics immediately or several hours after exposure to the polymer. The properties of the cell progeny which survived the sublethal effects of PAA and resumed their proliferation, were not monitored. The present study demonstrated that treatment of immortalized Chinese hamster cells CHLV-79 RJK sensitive (RJK) and resistant (RJKEB) to ethidium bromide (EB) with cytotoxic doses of PAA, selected cells with increased karyotypic instability, were accompanied by changes in the expression of p53 genes c-fos, topo2-α, hsp90, hsc70. These changes did not contribute to the progression of MDR, accompanied by the increased sensitivity of these cells to the toxic effects of doxorubicin (DOX). Our results showed that PAA does not increase the oncogenic potential of immortalized cells and confirmed that it can be used for intracellular drug delivery for anticancer therapy. MDPI 2020-10-21 /pmc/articles/PMC7589997/ /pubmed/33096691 http://dx.doi.org/10.3390/cells9102332 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alekseenko, Larisa
Shilina, Mariia
Kozhukharova, Irina
Lyublinskaya, Olga
Fridlyanskaya, Irina
Nikolsky, Nikolay
Grinchuk, Tatiana
Impact of Polyallylamine Hydrochloride on Gene Expression and Karyotypic Stability of Multidrug Resistant Transformed Cells
title Impact of Polyallylamine Hydrochloride on Gene Expression and Karyotypic Stability of Multidrug Resistant Transformed Cells
title_full Impact of Polyallylamine Hydrochloride on Gene Expression and Karyotypic Stability of Multidrug Resistant Transformed Cells
title_fullStr Impact of Polyallylamine Hydrochloride on Gene Expression and Karyotypic Stability of Multidrug Resistant Transformed Cells
title_full_unstemmed Impact of Polyallylamine Hydrochloride on Gene Expression and Karyotypic Stability of Multidrug Resistant Transformed Cells
title_short Impact of Polyallylamine Hydrochloride on Gene Expression and Karyotypic Stability of Multidrug Resistant Transformed Cells
title_sort impact of polyallylamine hydrochloride on gene expression and karyotypic stability of multidrug resistant transformed cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7589997/
https://www.ncbi.nlm.nih.gov/pubmed/33096691
http://dx.doi.org/10.3390/cells9102332
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