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Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells

Vimentin, an intermediate filament protein present in leukocytes, blood vessel endothelial cells, and multiple mesenchymal cells, such as mouse embryonic fibroblasts (MEF 3T3), is crucial for various cellular processes, as well as for maintaining the integrity and durability (stability) of the cell...

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Autores principales: Feliksiak, Karolina, Witko, Tomasz, Solarz, Daria, Guzik, Maciej, Rajfur, Zenon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590159/
https://www.ncbi.nlm.nih.gov/pubmed/33050497
http://dx.doi.org/10.3390/ijms21207478
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author Feliksiak, Karolina
Witko, Tomasz
Solarz, Daria
Guzik, Maciej
Rajfur, Zenon
author_facet Feliksiak, Karolina
Witko, Tomasz
Solarz, Daria
Guzik, Maciej
Rajfur, Zenon
author_sort Feliksiak, Karolina
collection PubMed
description Vimentin, an intermediate filament protein present in leukocytes, blood vessel endothelial cells, and multiple mesenchymal cells, such as mouse embryonic fibroblasts (MEF 3T3), is crucial for various cellular processes, as well as for maintaining the integrity and durability (stability) of the cell cytoskeleton. Vimentin intermediate filaments (VIFs) adhere tightly to the nucleus and spread to the lamellipodium and tail of the cell, serving as a connector between the nucleus, and the cell’s edges, especially in terms of transferring mechanical signals throughout the cell. How these signals are transmitted exactly remains under investigation. In the presented work, we propose that vimentin is involved in that transition by influencing the shape of the nucleus through the formation of nuclear blebs and grooves, as demonstrated by microscopic observations of healthy MEF (3T3) cells. Grooved, or “coffee beans” nuclei, have, to date, been noticed in several healthy cells; however, these structures are especially frequent in cancer cells—they serve as a significant marker for recognition of multiple cancers. We observed 288 MEF3T3 cells cultured on polyhydroxyoctanoate (PHO), polylactide (PLA), and glass, and we identified grooves, coaligned with vimentin fibers in the nuclei of 47% of cells cultured on PHO, 50% of cells on glass, and 59% of cells growing on PLA. We also observed nuclear blebs and associated their occurrence with the type of substrate used for cell culture. We propose that the higher rate of blebs in the nuclei of cells, cultured on PLA, is related to the microenvironmental features of the substrate, pH in particular.
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spelling pubmed-75901592020-10-29 Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells Feliksiak, Karolina Witko, Tomasz Solarz, Daria Guzik, Maciej Rajfur, Zenon Int J Mol Sci Article Vimentin, an intermediate filament protein present in leukocytes, blood vessel endothelial cells, and multiple mesenchymal cells, such as mouse embryonic fibroblasts (MEF 3T3), is crucial for various cellular processes, as well as for maintaining the integrity and durability (stability) of the cell cytoskeleton. Vimentin intermediate filaments (VIFs) adhere tightly to the nucleus and spread to the lamellipodium and tail of the cell, serving as a connector between the nucleus, and the cell’s edges, especially in terms of transferring mechanical signals throughout the cell. How these signals are transmitted exactly remains under investigation. In the presented work, we propose that vimentin is involved in that transition by influencing the shape of the nucleus through the formation of nuclear blebs and grooves, as demonstrated by microscopic observations of healthy MEF (3T3) cells. Grooved, or “coffee beans” nuclei, have, to date, been noticed in several healthy cells; however, these structures are especially frequent in cancer cells—they serve as a significant marker for recognition of multiple cancers. We observed 288 MEF3T3 cells cultured on polyhydroxyoctanoate (PHO), polylactide (PLA), and glass, and we identified grooves, coaligned with vimentin fibers in the nuclei of 47% of cells cultured on PHO, 50% of cells on glass, and 59% of cells growing on PLA. We also observed nuclear blebs and associated their occurrence with the type of substrate used for cell culture. We propose that the higher rate of blebs in the nuclei of cells, cultured on PLA, is related to the microenvironmental features of the substrate, pH in particular. MDPI 2020-10-10 /pmc/articles/PMC7590159/ /pubmed/33050497 http://dx.doi.org/10.3390/ijms21207478 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feliksiak, Karolina
Witko, Tomasz
Solarz, Daria
Guzik, Maciej
Rajfur, Zenon
Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells
title Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells
title_full Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells
title_fullStr Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells
title_full_unstemmed Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells
title_short Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells
title_sort vimentin association with nuclear grooves in normal mef 3t3 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590159/
https://www.ncbi.nlm.nih.gov/pubmed/33050497
http://dx.doi.org/10.3390/ijms21207478
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