Cargando…
Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells
Adult human cardiac mesenchymal progenitor cells (hCmPC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Even if the mechanisms have not yet been fully elucidated, the stem cell differentiation is guided by the mitochondrial metabolism; however, mitochondrial a...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590175/ https://www.ncbi.nlm.nih.gov/pubmed/33050449 http://dx.doi.org/10.3390/ijms21207467 |
_version_ | 1783600747461476352 |
---|---|
author | Gambini, Elisa Martinelli, Ilenia Stadiotti, Ilaria Vinci, Maria Cristina Scopece, Alessandro Eramo, Luana Sommariva, Elena Resta, Jessica Benaouadi, Sabrina Cogliati, Elisa Paolin, Adolfo Parini, Angelo Pompilio, Giulio Savagner, Frederique |
author_facet | Gambini, Elisa Martinelli, Ilenia Stadiotti, Ilaria Vinci, Maria Cristina Scopece, Alessandro Eramo, Luana Sommariva, Elena Resta, Jessica Benaouadi, Sabrina Cogliati, Elisa Paolin, Adolfo Parini, Angelo Pompilio, Giulio Savagner, Frederique |
author_sort | Gambini, Elisa |
collection | PubMed |
description | Adult human cardiac mesenchymal progenitor cells (hCmPC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Even if the mechanisms have not yet been fully elucidated, the stem cell differentiation is guided by the mitochondrial metabolism; however, mitochondrial approaches to identify hCmPC with enhanced stemness and/or differentiation capability for cellular therapy are not established. Here we demonstrated that hCmPCs sorted for low and high mitochondrial membrane potential (using a lipophilic cationic dye tetramethylrhodamine methyl ester, TMRM), presented differences in energy metabolism from preferential glycolysis to oxidative rates. TMRM-high cells are highly efficient in terms of oxygen consumption rate, basal and maximal respiration, and spare respiratory capacity compared to TMRM-low cells. TMRM-high cells showed characteristics of pre-committed cells and were associated with higher in vitro differentiation capacity through endothelial, cardiac-like, and, to a lesser extent, adipogenic and chondro/osteogenic cell lineage, when compared with TMRM-low cells. Conversely, TMRM-low showed higher self-renewal potential. To conclude, we identified two hCmPC populations with different metabolic profile, stemness maturity, and differentiation potential. Our findings suggest that metabolic sorting can isolate cells with higher regenerative capacity and/or long-term survival. This metabolism-based strategy to select cells may be broadly applicable to therapies. |
format | Online Article Text |
id | pubmed-7590175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75901752020-10-29 Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells Gambini, Elisa Martinelli, Ilenia Stadiotti, Ilaria Vinci, Maria Cristina Scopece, Alessandro Eramo, Luana Sommariva, Elena Resta, Jessica Benaouadi, Sabrina Cogliati, Elisa Paolin, Adolfo Parini, Angelo Pompilio, Giulio Savagner, Frederique Int J Mol Sci Article Adult human cardiac mesenchymal progenitor cells (hCmPC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Even if the mechanisms have not yet been fully elucidated, the stem cell differentiation is guided by the mitochondrial metabolism; however, mitochondrial approaches to identify hCmPC with enhanced stemness and/or differentiation capability for cellular therapy are not established. Here we demonstrated that hCmPCs sorted for low and high mitochondrial membrane potential (using a lipophilic cationic dye tetramethylrhodamine methyl ester, TMRM), presented differences in energy metabolism from preferential glycolysis to oxidative rates. TMRM-high cells are highly efficient in terms of oxygen consumption rate, basal and maximal respiration, and spare respiratory capacity compared to TMRM-low cells. TMRM-high cells showed characteristics of pre-committed cells and were associated with higher in vitro differentiation capacity through endothelial, cardiac-like, and, to a lesser extent, adipogenic and chondro/osteogenic cell lineage, when compared with TMRM-low cells. Conversely, TMRM-low showed higher self-renewal potential. To conclude, we identified two hCmPC populations with different metabolic profile, stemness maturity, and differentiation potential. Our findings suggest that metabolic sorting can isolate cells with higher regenerative capacity and/or long-term survival. This metabolism-based strategy to select cells may be broadly applicable to therapies. MDPI 2020-10-10 /pmc/articles/PMC7590175/ /pubmed/33050449 http://dx.doi.org/10.3390/ijms21207467 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gambini, Elisa Martinelli, Ilenia Stadiotti, Ilaria Vinci, Maria Cristina Scopece, Alessandro Eramo, Luana Sommariva, Elena Resta, Jessica Benaouadi, Sabrina Cogliati, Elisa Paolin, Adolfo Parini, Angelo Pompilio, Giulio Savagner, Frederique Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells |
title | Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells |
title_full | Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells |
title_fullStr | Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells |
title_full_unstemmed | Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells |
title_short | Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells |
title_sort | differences in mitochondrial membrane potential identify distinct populations of human cardiac mesenchymal progenitor cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590175/ https://www.ncbi.nlm.nih.gov/pubmed/33050449 http://dx.doi.org/10.3390/ijms21207467 |
work_keys_str_mv | AT gambinielisa differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT martinelliilenia differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT stadiottiilaria differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT vincimariacristina differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT scopecealessandro differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT eramoluana differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT sommarivaelena differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT restajessica differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT benaouadisabrina differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT cogliatielisa differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT paolinadolfo differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT pariniangelo differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT pompiliogiulio differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells AT savagnerfrederique differencesinmitochondrialmembranepotentialidentifydistinctpopulationsofhumancardiacmesenchymalprogenitorcells |