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Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts
We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane biosynthesis) and vacuole formation during the enlar...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shared Science Publishers OG
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590531/ https://www.ncbi.nlm.nih.gov/pubmed/33150162 http://dx.doi.org/10.15698/mic2020.11.735 |
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author | Tsuchikado, Rintaro Kami, Satoshi Takahashi, Sawako Nishida, Hiromi |
author_facet | Tsuchikado, Rintaro Kami, Satoshi Takahashi, Sawako Nishida, Hiromi |
author_sort | Tsuchikado, Rintaro |
collection | PubMed |
description | We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane biosynthesis) and vacuole formation during the enlargement of the E. faecalis protoplasts. After novobiocin treatment prior to vacuole formation, the cell size of E. faecalis protoplasts was limited to 6 μm in diameter and the cells lacked vacuoles. When novobiocin was added after vacuole formation, E. faecalis protoplasts grew with vacuole enlargement; after novobiocin removal, protoplasts were enlarged again. Although cell size distribution of the protoplasts was similar following the 24 h and 48 h novobiocin treatments, after 72 h of novobiocin treatment there was a greater number of smaller sized protoplasts, suggesting that extended novobiocin treatment may inhibit the re-enlargement of E. faecalis protoplasts after novobiocin removal. Our findings demonstrate that novobiocin can control the enlargement of E. faecalis protoplasts due to inhibition of DNA replication. |
format | Online Article Text |
id | pubmed-7590531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Shared Science Publishers OG |
record_format | MEDLINE/PubMed |
spelling | pubmed-75905312020-11-03 Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts Tsuchikado, Rintaro Kami, Satoshi Takahashi, Sawako Nishida, Hiromi Microb Cell Research Article We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane biosynthesis) and vacuole formation during the enlargement of the E. faecalis protoplasts. After novobiocin treatment prior to vacuole formation, the cell size of E. faecalis protoplasts was limited to 6 μm in diameter and the cells lacked vacuoles. When novobiocin was added after vacuole formation, E. faecalis protoplasts grew with vacuole enlargement; after novobiocin removal, protoplasts were enlarged again. Although cell size distribution of the protoplasts was similar following the 24 h and 48 h novobiocin treatments, after 72 h of novobiocin treatment there was a greater number of smaller sized protoplasts, suggesting that extended novobiocin treatment may inhibit the re-enlargement of E. faecalis protoplasts after novobiocin removal. Our findings demonstrate that novobiocin can control the enlargement of E. faecalis protoplasts due to inhibition of DNA replication. Shared Science Publishers OG 2020-08-10 /pmc/articles/PMC7590531/ /pubmed/33150162 http://dx.doi.org/10.15698/mic2020.11.735 Text en Copyright: © 2020 Tsuchikado et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged. |
spellingShingle | Research Article Tsuchikado, Rintaro Kami, Satoshi Takahashi, Sawako Nishida, Hiromi Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts |
title | Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts |
title_full | Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts |
title_fullStr | Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts |
title_full_unstemmed | Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts |
title_short | Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts |
title_sort | novobiocin inhibits membrane synthesis and vacuole formation of enterococcus faecalis protoplasts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590531/ https://www.ncbi.nlm.nih.gov/pubmed/33150162 http://dx.doi.org/10.15698/mic2020.11.735 |
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