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Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts

We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane biosynthesis) and vacuole formation during the enlar...

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Autores principales: Tsuchikado, Rintaro, Kami, Satoshi, Takahashi, Sawako, Nishida, Hiromi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shared Science Publishers OG 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590531/
https://www.ncbi.nlm.nih.gov/pubmed/33150162
http://dx.doi.org/10.15698/mic2020.11.735
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author Tsuchikado, Rintaro
Kami, Satoshi
Takahashi, Sawako
Nishida, Hiromi
author_facet Tsuchikado, Rintaro
Kami, Satoshi
Takahashi, Sawako
Nishida, Hiromi
author_sort Tsuchikado, Rintaro
collection PubMed
description We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane biosynthesis) and vacuole formation during the enlargement of the E. faecalis protoplasts. After novobiocin treatment prior to vacuole formation, the cell size of E. faecalis protoplasts was limited to 6 μm in diameter and the cells lacked vacuoles. When novobiocin was added after vacuole formation, E. faecalis protoplasts grew with vacuole enlargement; after novobiocin removal, protoplasts were enlarged again. Although cell size distribution of the protoplasts was similar following the 24 h and 48 h novobiocin treatments, after 72 h of novobiocin treatment there was a greater number of smaller sized protoplasts, suggesting that extended novobiocin treatment may inhibit the re-enlargement of E. faecalis protoplasts after novobiocin removal. Our findings demonstrate that novobiocin can control the enlargement of E. faecalis protoplasts due to inhibition of DNA replication.
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spelling pubmed-75905312020-11-03 Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts Tsuchikado, Rintaro Kami, Satoshi Takahashi, Sawako Nishida, Hiromi Microb Cell Research Article We demonstrate that plasma membrane biosynthesis and vacuole formation require DNA replication in Enterococcus faecalis protoplasts. The replication inhibitor novobiocin inhibited not only DNA replication but also cell enlargement (plasma membrane biosynthesis) and vacuole formation during the enlargement of the E. faecalis protoplasts. After novobiocin treatment prior to vacuole formation, the cell size of E. faecalis protoplasts was limited to 6 μm in diameter and the cells lacked vacuoles. When novobiocin was added after vacuole formation, E. faecalis protoplasts grew with vacuole enlargement; after novobiocin removal, protoplasts were enlarged again. Although cell size distribution of the protoplasts was similar following the 24 h and 48 h novobiocin treatments, after 72 h of novobiocin treatment there was a greater number of smaller sized protoplasts, suggesting that extended novobiocin treatment may inhibit the re-enlargement of E. faecalis protoplasts after novobiocin removal. Our findings demonstrate that novobiocin can control the enlargement of E. faecalis protoplasts due to inhibition of DNA replication. Shared Science Publishers OG 2020-08-10 /pmc/articles/PMC7590531/ /pubmed/33150162 http://dx.doi.org/10.15698/mic2020.11.735 Text en Copyright: © 2020 Tsuchikado et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
spellingShingle Research Article
Tsuchikado, Rintaro
Kami, Satoshi
Takahashi, Sawako
Nishida, Hiromi
Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts
title Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts
title_full Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts
title_fullStr Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts
title_full_unstemmed Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts
title_short Novobiocin inhibits membrane synthesis and vacuole formation of Enterococcus faecalis protoplasts
title_sort novobiocin inhibits membrane synthesis and vacuole formation of enterococcus faecalis protoplasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590531/
https://www.ncbi.nlm.nih.gov/pubmed/33150162
http://dx.doi.org/10.15698/mic2020.11.735
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