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Chromatin accessibility dynamics of Chlamydia-infected epithelial cells

Chlamydia are Gram-negative, obligate intracellular bacterial pathogens responsible for a broad spectrum of human and animal diseases. In humans, Chlamydia trachomatis is the most prevalent bacterial sexually transmitted infection worldwide and is the causative agent of trachoma (infectious blindnes...

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Autores principales: Hayward, Regan J., Marsh, James W., Humphrys, Michael S., Huston, Wilhelmina M., Myers, Garry S. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590614/
https://www.ncbi.nlm.nih.gov/pubmed/33109274
http://dx.doi.org/10.1186/s13072-020-00368-2
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author Hayward, Regan J.
Marsh, James W.
Humphrys, Michael S.
Huston, Wilhelmina M.
Myers, Garry S. A.
author_facet Hayward, Regan J.
Marsh, James W.
Humphrys, Michael S.
Huston, Wilhelmina M.
Myers, Garry S. A.
author_sort Hayward, Regan J.
collection PubMed
description Chlamydia are Gram-negative, obligate intracellular bacterial pathogens responsible for a broad spectrum of human and animal diseases. In humans, Chlamydia trachomatis is the most prevalent bacterial sexually transmitted infection worldwide and is the causative agent of trachoma (infectious blindness) in disadvantaged populations. Over the course of its developmental cycle, Chlamydia extensively remodels its intracellular niche and parasitises the host cell for nutrients, with substantial resulting changes to the host cell transcriptome and proteome. However, little information is available on the impact of chlamydial infection on the host cell epigenome and global gene regulation. Regions of open eukaryotic chromatin correspond to nucleosome-depleted regions, which in turn are associated with regulatory functions and transcription factor binding. We applied formaldehyde-assisted isolation of regulatory elements enrichment followed by sequencing (FAIRE-Seq) to generate temporal chromatin maps of C. trachomatis-infected human epithelial cells in vitro over the chlamydial developmental cycle. We detected both conserved and distinct temporal changes to genome-wide chromatin accessibility associated with C. trachomatis infection. The observed differentially accessible chromatin regions include temporally-enriched sets of transcription factors, which may help shape the host cell response to infection. These regions and motifs were linked to genomic features and genes associated with immune responses, re-direction of host cell nutrients, intracellular signalling, cell–cell adhesion, extracellular matrix, metabolism and apoptosis. This work provides another perspective to the complex response to chlamydial infection, and will inform further studies of transcriptional regulation and the epigenome in Chlamydia-infected human cells and tissues.
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spelling pubmed-75906142020-10-27 Chromatin accessibility dynamics of Chlamydia-infected epithelial cells Hayward, Regan J. Marsh, James W. Humphrys, Michael S. Huston, Wilhelmina M. Myers, Garry S. A. Epigenetics Chromatin Research Chlamydia are Gram-negative, obligate intracellular bacterial pathogens responsible for a broad spectrum of human and animal diseases. In humans, Chlamydia trachomatis is the most prevalent bacterial sexually transmitted infection worldwide and is the causative agent of trachoma (infectious blindness) in disadvantaged populations. Over the course of its developmental cycle, Chlamydia extensively remodels its intracellular niche and parasitises the host cell for nutrients, with substantial resulting changes to the host cell transcriptome and proteome. However, little information is available on the impact of chlamydial infection on the host cell epigenome and global gene regulation. Regions of open eukaryotic chromatin correspond to nucleosome-depleted regions, which in turn are associated with regulatory functions and transcription factor binding. We applied formaldehyde-assisted isolation of regulatory elements enrichment followed by sequencing (FAIRE-Seq) to generate temporal chromatin maps of C. trachomatis-infected human epithelial cells in vitro over the chlamydial developmental cycle. We detected both conserved and distinct temporal changes to genome-wide chromatin accessibility associated with C. trachomatis infection. The observed differentially accessible chromatin regions include temporally-enriched sets of transcription factors, which may help shape the host cell response to infection. These regions and motifs were linked to genomic features and genes associated with immune responses, re-direction of host cell nutrients, intracellular signalling, cell–cell adhesion, extracellular matrix, metabolism and apoptosis. This work provides another perspective to the complex response to chlamydial infection, and will inform further studies of transcriptional regulation and the epigenome in Chlamydia-infected human cells and tissues. BioMed Central 2020-10-27 /pmc/articles/PMC7590614/ /pubmed/33109274 http://dx.doi.org/10.1186/s13072-020-00368-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Hayward, Regan J.
Marsh, James W.
Humphrys, Michael S.
Huston, Wilhelmina M.
Myers, Garry S. A.
Chromatin accessibility dynamics of Chlamydia-infected epithelial cells
title Chromatin accessibility dynamics of Chlamydia-infected epithelial cells
title_full Chromatin accessibility dynamics of Chlamydia-infected epithelial cells
title_fullStr Chromatin accessibility dynamics of Chlamydia-infected epithelial cells
title_full_unstemmed Chromatin accessibility dynamics of Chlamydia-infected epithelial cells
title_short Chromatin accessibility dynamics of Chlamydia-infected epithelial cells
title_sort chromatin accessibility dynamics of chlamydia-infected epithelial cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590614/
https://www.ncbi.nlm.nih.gov/pubmed/33109274
http://dx.doi.org/10.1186/s13072-020-00368-2
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