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Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures
Recently, the microbiome-derived trimethylamine-N-oxide (TMAO) was shown to be present in human cerebrospinal fluid (CSF). However, data on the potential of TMAO crossing the blood-CSF barrier are still lacking. This retrospective study aimed at investigating possible associations between the CSF/se...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Leibniz Research Centre for Working Environment and Human Factors
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590830/ https://www.ncbi.nlm.nih.gov/pubmed/33122976 http://dx.doi.org/10.17179/excli2020-2763 |
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author | Enko, Dietmar Zelzer, Sieglinde Niedrist, Tobias Holasek, Sandra Baranyi, Andreas Schnedl, Wolfgang J. Herrmann, Markus Meinitzer, Andreas |
author_facet | Enko, Dietmar Zelzer, Sieglinde Niedrist, Tobias Holasek, Sandra Baranyi, Andreas Schnedl, Wolfgang J. Herrmann, Markus Meinitzer, Andreas |
author_sort | Enko, Dietmar |
collection | PubMed |
description | Recently, the microbiome-derived trimethylamine-N-oxide (TMAO) was shown to be present in human cerebrospinal fluid (CSF). However, data on the potential of TMAO crossing the blood-CSF barrier are still lacking. This retrospective study aimed at investigating possible associations between the CSF/serum albumin (Q(ALB)) and TMAO (Q(TMAO)) quotient and evaluating Q(TMAO )values in individuals with and without blood-CSF barrier dysfunction. A total of 290 patients, who underwent diagnostic lumbar puncture with Q(ALB) and Q(TMAO )determination, were evaluated. Serum and CSF TMAO measurements were performed on a tandem mass spectrometry SCIEX QTRAP 4500 (Applied Biosystems, Framingham, MA, USA) coupled with an Agilent 1260 Infinity HPLC system (Agilent Technologies Santa Clara, CA, USA). Serum and CSF albumin were measured on the Atellica(® )NEPH 630 system (Siemens Healthineers, Erlangen, Germany). CSF TMAO levels were positively correlated with serum TMAO levels (ρ = 0.709, p < 0.001). The Q(ALB )was significantly associated with the Q(TMAO )(ß-coefficient = 0.312; p < 0.001). A total of 117 patients with blood-CSF barrier dysfunction had significantly higher median (Q1 - Q3) Q(TMAO )values (4.7 (2.8 - 7.5) vs. 3.8 (2.5 - 5.7) x 10(-1), p = 0.002) compared to 173 individuals with normal blood-CSF barrier function. CSF and serum TMAO concentrations were significantly associated in 290 CSF/serum pairs from lumbar punctures of clinical routine. Q(ALB )showed a relevant influence on Q(TMAO). Present results indicate that TMAO may cross the blood-CSF barrier. |
format | Online Article Text |
id | pubmed-7590830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Leibniz Research Centre for Working Environment and Human Factors |
record_format | MEDLINE/PubMed |
spelling | pubmed-75908302020-10-28 Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures Enko, Dietmar Zelzer, Sieglinde Niedrist, Tobias Holasek, Sandra Baranyi, Andreas Schnedl, Wolfgang J. Herrmann, Markus Meinitzer, Andreas EXCLI J Original Article Recently, the microbiome-derived trimethylamine-N-oxide (TMAO) was shown to be present in human cerebrospinal fluid (CSF). However, data on the potential of TMAO crossing the blood-CSF barrier are still lacking. This retrospective study aimed at investigating possible associations between the CSF/serum albumin (Q(ALB)) and TMAO (Q(TMAO)) quotient and evaluating Q(TMAO )values in individuals with and without blood-CSF barrier dysfunction. A total of 290 patients, who underwent diagnostic lumbar puncture with Q(ALB) and Q(TMAO )determination, were evaluated. Serum and CSF TMAO measurements were performed on a tandem mass spectrometry SCIEX QTRAP 4500 (Applied Biosystems, Framingham, MA, USA) coupled with an Agilent 1260 Infinity HPLC system (Agilent Technologies Santa Clara, CA, USA). Serum and CSF albumin were measured on the Atellica(® )NEPH 630 system (Siemens Healthineers, Erlangen, Germany). CSF TMAO levels were positively correlated with serum TMAO levels (ρ = 0.709, p < 0.001). The Q(ALB )was significantly associated with the Q(TMAO )(ß-coefficient = 0.312; p < 0.001). A total of 117 patients with blood-CSF barrier dysfunction had significantly higher median (Q1 - Q3) Q(TMAO )values (4.7 (2.8 - 7.5) vs. 3.8 (2.5 - 5.7) x 10(-1), p = 0.002) compared to 173 individuals with normal blood-CSF barrier function. CSF and serum TMAO concentrations were significantly associated in 290 CSF/serum pairs from lumbar punctures of clinical routine. Q(ALB )showed a relevant influence on Q(TMAO). Present results indicate that TMAO may cross the blood-CSF barrier. Leibniz Research Centre for Working Environment and Human Factors 2020-09-09 /pmc/articles/PMC7590830/ /pubmed/33122976 http://dx.doi.org/10.17179/excli2020-2763 Text en Copyright © 2020 Enko et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/) You are free to copy, distribute and transmit the work, provided the original author and source are credited. |
spellingShingle | Original Article Enko, Dietmar Zelzer, Sieglinde Niedrist, Tobias Holasek, Sandra Baranyi, Andreas Schnedl, Wolfgang J. Herrmann, Markus Meinitzer, Andreas Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures |
title | Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures |
title_full | Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures |
title_fullStr | Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures |
title_full_unstemmed | Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures |
title_short | Assessment of trimethylamine-N-oxide at the blood-cerebrospinal fluid barrier: Results from 290 lumbar punctures |
title_sort | assessment of trimethylamine-n-oxide at the blood-cerebrospinal fluid barrier: results from 290 lumbar punctures |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590830/ https://www.ncbi.nlm.nih.gov/pubmed/33122976 http://dx.doi.org/10.17179/excli2020-2763 |
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