Cargando…
Pre-exposure to mechanical ventilation and endotoxemia increases Pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia
BACKGROUND: Immune system suppression during critical care contributes to the risk of acquired bacterial infections with Pseudomonas (P.) aeruginosa. Repeated exposure to endotoxin can attenuate systemic inflammatory cytokine responses. Mechanical ventilation affects the systemic inflammatory respon...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591049/ https://www.ncbi.nlm.nih.gov/pubmed/33108383 http://dx.doi.org/10.1371/journal.pone.0240753 |
_version_ | 1783600916130168832 |
---|---|
author | Sperber, Jesper Nyberg, Axel Krifors, Anders Skorup, Paul Lipcsey, Miklós Castegren, Markus |
author_facet | Sperber, Jesper Nyberg, Axel Krifors, Anders Skorup, Paul Lipcsey, Miklós Castegren, Markus |
author_sort | Sperber, Jesper |
collection | PubMed |
description | BACKGROUND: Immune system suppression during critical care contributes to the risk of acquired bacterial infections with Pseudomonas (P.) aeruginosa. Repeated exposure to endotoxin can attenuate systemic inflammatory cytokine responses. Mechanical ventilation affects the systemic inflammatory response to various stimuli. AIM: To study the effect of pre-exposure to mechanical ventilation with and without endotoxin-induced systemic inflammation on P. aeruginosa growth and wet-to-dry weight measurements on lung tissue and plasma and bronchoalveolar lavage levels of tumor necrosis factor alpha, interleukins 6 and 10. METHODS: Two groups of pigs were exposed to mechanical ventilation for 24 hours before bacterial inoculation and six h of experimental pneumonia (total experimental time 30 h): A(30h+Etx) (n = 6, endotoxin 0.063 μg x kg(-1) x h(-1)) and B(30h) (n = 6, saline). A third group, C(6h) (n = 8), started the experiment at the bacterial inoculation unexposed to endotoxin or mechanical ventilation (total experimental time 6 h). Bacterial inoculation was performed by tracheal instillation of 1x10(11) colony-forming units of P. aeruginosa. Bacterial cultures and wet-to-dry weight ratio analyses were done on lung tissue samples postmortem. Separate group comparisons were done between A(30h+Etx) vs.B(30h) (Inflammation) and B(30h) vs. C(6h) (Ventilation Time) during the bacterial phase of 6 h. RESULTS: P. aeruginosa growth was highest in A(30h+Etx), and lowest in C(6h) (Inflammation and Ventilation Time both p<0.05). Lung wet-to-dry weight ratios were highest in A(30h+Etx) and lowest in B(30h) (Inflammation p<0.01, Ventilation Time p<0.05). C(6h) had the highest TNF-α levels in plasma (Ventilation Time p<0.01). No differences in bronchoalveolar lavage variables between the groups were observed. CONCLUSIONS: Mechanical ventilation and systemic inflammation before the onset of pneumonia increase the growth of P. aeruginosa in lung tissue. The attenuated growth of P. aeruginosa in the non-pre-exposed animals (C(6h)) was associated with a higher systemic TNF-α production elicited from the bacterial challenge. |
format | Online Article Text |
id | pubmed-7591049 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-75910492020-10-30 Pre-exposure to mechanical ventilation and endotoxemia increases Pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia Sperber, Jesper Nyberg, Axel Krifors, Anders Skorup, Paul Lipcsey, Miklós Castegren, Markus PLoS One Research Article BACKGROUND: Immune system suppression during critical care contributes to the risk of acquired bacterial infections with Pseudomonas (P.) aeruginosa. Repeated exposure to endotoxin can attenuate systemic inflammatory cytokine responses. Mechanical ventilation affects the systemic inflammatory response to various stimuli. AIM: To study the effect of pre-exposure to mechanical ventilation with and without endotoxin-induced systemic inflammation on P. aeruginosa growth and wet-to-dry weight measurements on lung tissue and plasma and bronchoalveolar lavage levels of tumor necrosis factor alpha, interleukins 6 and 10. METHODS: Two groups of pigs were exposed to mechanical ventilation for 24 hours before bacterial inoculation and six h of experimental pneumonia (total experimental time 30 h): A(30h+Etx) (n = 6, endotoxin 0.063 μg x kg(-1) x h(-1)) and B(30h) (n = 6, saline). A third group, C(6h) (n = 8), started the experiment at the bacterial inoculation unexposed to endotoxin or mechanical ventilation (total experimental time 6 h). Bacterial inoculation was performed by tracheal instillation of 1x10(11) colony-forming units of P. aeruginosa. Bacterial cultures and wet-to-dry weight ratio analyses were done on lung tissue samples postmortem. Separate group comparisons were done between A(30h+Etx) vs.B(30h) (Inflammation) and B(30h) vs. C(6h) (Ventilation Time) during the bacterial phase of 6 h. RESULTS: P. aeruginosa growth was highest in A(30h+Etx), and lowest in C(6h) (Inflammation and Ventilation Time both p<0.05). Lung wet-to-dry weight ratios were highest in A(30h+Etx) and lowest in B(30h) (Inflammation p<0.01, Ventilation Time p<0.05). C(6h) had the highest TNF-α levels in plasma (Ventilation Time p<0.01). No differences in bronchoalveolar lavage variables between the groups were observed. CONCLUSIONS: Mechanical ventilation and systemic inflammation before the onset of pneumonia increase the growth of P. aeruginosa in lung tissue. The attenuated growth of P. aeruginosa in the non-pre-exposed animals (C(6h)) was associated with a higher systemic TNF-α production elicited from the bacterial challenge. Public Library of Science 2020-10-27 /pmc/articles/PMC7591049/ /pubmed/33108383 http://dx.doi.org/10.1371/journal.pone.0240753 Text en © 2020 Sperber et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sperber, Jesper Nyberg, Axel Krifors, Anders Skorup, Paul Lipcsey, Miklós Castegren, Markus Pre-exposure to mechanical ventilation and endotoxemia increases Pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia |
title | Pre-exposure to mechanical ventilation and endotoxemia increases Pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia |
title_full | Pre-exposure to mechanical ventilation and endotoxemia increases Pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia |
title_fullStr | Pre-exposure to mechanical ventilation and endotoxemia increases Pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia |
title_full_unstemmed | Pre-exposure to mechanical ventilation and endotoxemia increases Pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia |
title_short | Pre-exposure to mechanical ventilation and endotoxemia increases Pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia |
title_sort | pre-exposure to mechanical ventilation and endotoxemia increases pseudomonas aeruginosa growth in lung tissue during experimental porcine pneumonia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591049/ https://www.ncbi.nlm.nih.gov/pubmed/33108383 http://dx.doi.org/10.1371/journal.pone.0240753 |
work_keys_str_mv | AT sperberjesper preexposuretomechanicalventilationandendotoxemiaincreasespseudomonasaeruginosagrowthinlungtissueduringexperimentalporcinepneumonia AT nybergaxel preexposuretomechanicalventilationandendotoxemiaincreasespseudomonasaeruginosagrowthinlungtissueduringexperimentalporcinepneumonia AT kriforsanders preexposuretomechanicalventilationandendotoxemiaincreasespseudomonasaeruginosagrowthinlungtissueduringexperimentalporcinepneumonia AT skoruppaul preexposuretomechanicalventilationandendotoxemiaincreasespseudomonasaeruginosagrowthinlungtissueduringexperimentalporcinepneumonia AT lipcseymiklos preexposuretomechanicalventilationandendotoxemiaincreasespseudomonasaeruginosagrowthinlungtissueduringexperimentalporcinepneumonia AT castegrenmarkus preexposuretomechanicalventilationandendotoxemiaincreasespseudomonasaeruginosagrowthinlungtissueduringexperimentalporcinepneumonia |