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Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA
Intestinal worms, or soil-transmitted helminths (STHs), affect hundreds of millions of people in all tropical and subtropical regions of the world. The most prevalent STH is Ascaris lumbricoides. Through large-scale deworming programs, World Health Organization aims to reduce morbidity, caused by mo...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591086/ https://www.ncbi.nlm.nih.gov/pubmed/33057357 http://dx.doi.org/10.1371/journal.pntd.0008807 |
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author | Lagatie, Ole Verheyen, Ann Van Hoof, Kim Lauwers, Dax Odiere, Maurice R. Vlaminck, Johnny Levecke, Bruno Stuyver, Lieven J. |
author_facet | Lagatie, Ole Verheyen, Ann Van Hoof, Kim Lauwers, Dax Odiere, Maurice R. Vlaminck, Johnny Levecke, Bruno Stuyver, Lieven J. |
author_sort | Lagatie, Ole |
collection | PubMed |
description | Intestinal worms, or soil-transmitted helminths (STHs), affect hundreds of millions of people in all tropical and subtropical regions of the world. The most prevalent STH is Ascaris lumbricoides. Through large-scale deworming programs, World Health Organization aims to reduce morbidity, caused by moderate-to-heavy intensity infections, below 2%. In order to monitor these control programs, stool samples are examined microscopically for the presence of worm eggs. This procedure requires well-trained personnel and is known to show variability between different operators interpreting the slides. We have investigated whether ABA-1, one of the excretory-secretory products of A. lumbricoides can be used as a coproantigen marker for infection with this parasite. Polyclonal antibodies were generated and a coproantigen ELISA was developed. Using this ELISA, it was found that ABA-1 in stool detected Ascaris infection with a sensitivity of 91.5% and a specificity of 95.3%. Our results also demonstrate that there is a correlation between ABA-1 levels in stool and A. lumbricoides DNA detected in stool. Using a threshold of 18.2 ng/g stool the ABA-1 ELISA correctly assigned 68.4% of infected individuals to the moderate-to-heavy intensity infection group, with a specificity of 97.1%. Furthermore, the levels of ABA-1 in stool were shown to rapidly and strongly decrease upon administration of a standard anthelminthic treatment (single oral dose of 400 mg albendazole). In an Ascaris suum infection model in pigs, it was found that ABA-1 remained undetectable until day 28 and was detected at day 42 or 56, concurrent with the appearance of worm eggs in the stool. This report demonstrates that ABA-1 can be considered an Ascaris -specific coproantigen marker that can be used to monitor infection intensity. It also opens the path for development of point-of-care immunoassay-based tests to determine A. lumbricoides infection in stool at the sample collection site. |
format | Online Article Text |
id | pubmed-7591086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-75910862020-10-30 Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA Lagatie, Ole Verheyen, Ann Van Hoof, Kim Lauwers, Dax Odiere, Maurice R. Vlaminck, Johnny Levecke, Bruno Stuyver, Lieven J. PLoS Negl Trop Dis Research Article Intestinal worms, or soil-transmitted helminths (STHs), affect hundreds of millions of people in all tropical and subtropical regions of the world. The most prevalent STH is Ascaris lumbricoides. Through large-scale deworming programs, World Health Organization aims to reduce morbidity, caused by moderate-to-heavy intensity infections, below 2%. In order to monitor these control programs, stool samples are examined microscopically for the presence of worm eggs. This procedure requires well-trained personnel and is known to show variability between different operators interpreting the slides. We have investigated whether ABA-1, one of the excretory-secretory products of A. lumbricoides can be used as a coproantigen marker for infection with this parasite. Polyclonal antibodies were generated and a coproantigen ELISA was developed. Using this ELISA, it was found that ABA-1 in stool detected Ascaris infection with a sensitivity of 91.5% and a specificity of 95.3%. Our results also demonstrate that there is a correlation between ABA-1 levels in stool and A. lumbricoides DNA detected in stool. Using a threshold of 18.2 ng/g stool the ABA-1 ELISA correctly assigned 68.4% of infected individuals to the moderate-to-heavy intensity infection group, with a specificity of 97.1%. Furthermore, the levels of ABA-1 in stool were shown to rapidly and strongly decrease upon administration of a standard anthelminthic treatment (single oral dose of 400 mg albendazole). In an Ascaris suum infection model in pigs, it was found that ABA-1 remained undetectable until day 28 and was detected at day 42 or 56, concurrent with the appearance of worm eggs in the stool. This report demonstrates that ABA-1 can be considered an Ascaris -specific coproantigen marker that can be used to monitor infection intensity. It also opens the path for development of point-of-care immunoassay-based tests to determine A. lumbricoides infection in stool at the sample collection site. Public Library of Science 2020-10-15 /pmc/articles/PMC7591086/ /pubmed/33057357 http://dx.doi.org/10.1371/journal.pntd.0008807 Text en © 2020 Lagatie et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lagatie, Ole Verheyen, Ann Van Hoof, Kim Lauwers, Dax Odiere, Maurice R. Vlaminck, Johnny Levecke, Bruno Stuyver, Lieven J. Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA |
title | Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA |
title_full | Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA |
title_fullStr | Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA |
title_full_unstemmed | Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA |
title_short | Detection of Ascaris lumbricoides infection by ABA-1 coproantigen ELISA |
title_sort | detection of ascaris lumbricoides infection by aba-1 coproantigen elisa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591086/ https://www.ncbi.nlm.nih.gov/pubmed/33057357 http://dx.doi.org/10.1371/journal.pntd.0008807 |
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