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Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression

AIM: This study was designed to use in vivo and in vitro approaches to evaluate puerarin in diabetes-induced renal injury. MATERIALS AND METHODS: SD rats were divided into NC (normal control), Model (diabetic induced renal injury model), SP-L (model rats treated with low-dose standard puerarin), SP-...

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Autores principales: Xu, Yanmei, Xiong, Yan, Xu, Chen, Xu, Chuanwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591269/
https://www.ncbi.nlm.nih.gov/pubmed/33122931
http://dx.doi.org/10.2147/DMSO.S273952
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author Xu, Yanmei
Xiong, Yan
Xu, Chen
Xu, Chuanwen
author_facet Xu, Yanmei
Xiong, Yan
Xu, Chen
Xu, Chuanwen
author_sort Xu, Yanmei
collection PubMed
description AIM: This study was designed to use in vivo and in vitro approaches to evaluate puerarin in diabetes-induced renal injury. MATERIALS AND METHODS: SD rats were divided into NC (normal control), Model (diabetic induced renal injury model), SP-L (model rats treated with low-dose standard puerarin), SP-M (model rats treated with middle-dose standard puerarin), and SP-H (model rats treated with high-dose standard puerarin) groups. We evaluated fasting blood-glucose (FBG), urinary albumin/creatinine ratio (UACR), body weight, and kidney index (KI) in the different groups. TNF-α, IL-1β, and IL-6 concentrations were measured using Elisa assays. HE staining and TUNEL assays were used to evaluate pathology and apoptosis in kidney tissues, respectively. Relative gene and protein expression was measured using RT-qPCR and Western blot assays. Apoptosis was measured using flow cytometry. The correlation between miRNA-145-5p and TLR4 was assessed using dual-luciferase reporter gene assays. RESULTS: The pathology and apoptosis cell number were deteriorate in Model group; TNF-α, IL-1β and IL-6 concentrations, FGB, UACR and KI were increased and body weight was depressed; meanwhile, relative gene and proteins expressions (miRNA-145-5p, TLR4, MyD88 and NF-κB p65) were significantly different in Model group in vivo and vitro study compared with NC group. SP treatment significantly improved the pathology and apoptosis levels in the tissues, as well as TNF-α, IL-1β and IL-6 concentrations, FGB, UACR, body weight, and KI. In vitro cell studies revealed that SP could prevent renal injury induced by diabetes through the miRNA-145-5p/TLR4 axis. CONCLUSION: SP prevents diabetes-induced renal damage via miRNA-145-5p overexpression and reduces TLR4/MyD88/NF-κB (p65) pathway activation in vitro and in vivo.
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spelling pubmed-75912692020-10-28 Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression Xu, Yanmei Xiong, Yan Xu, Chen Xu, Chuanwen Diabetes Metab Syndr Obes Original Research AIM: This study was designed to use in vivo and in vitro approaches to evaluate puerarin in diabetes-induced renal injury. MATERIALS AND METHODS: SD rats were divided into NC (normal control), Model (diabetic induced renal injury model), SP-L (model rats treated with low-dose standard puerarin), SP-M (model rats treated with middle-dose standard puerarin), and SP-H (model rats treated with high-dose standard puerarin) groups. We evaluated fasting blood-glucose (FBG), urinary albumin/creatinine ratio (UACR), body weight, and kidney index (KI) in the different groups. TNF-α, IL-1β, and IL-6 concentrations were measured using Elisa assays. HE staining and TUNEL assays were used to evaluate pathology and apoptosis in kidney tissues, respectively. Relative gene and protein expression was measured using RT-qPCR and Western blot assays. Apoptosis was measured using flow cytometry. The correlation between miRNA-145-5p and TLR4 was assessed using dual-luciferase reporter gene assays. RESULTS: The pathology and apoptosis cell number were deteriorate in Model group; TNF-α, IL-1β and IL-6 concentrations, FGB, UACR and KI were increased and body weight was depressed; meanwhile, relative gene and proteins expressions (miRNA-145-5p, TLR4, MyD88 and NF-κB p65) were significantly different in Model group in vivo and vitro study compared with NC group. SP treatment significantly improved the pathology and apoptosis levels in the tissues, as well as TNF-α, IL-1β and IL-6 concentrations, FGB, UACR, body weight, and KI. In vitro cell studies revealed that SP could prevent renal injury induced by diabetes through the miRNA-145-5p/TLR4 axis. CONCLUSION: SP prevents diabetes-induced renal damage via miRNA-145-5p overexpression and reduces TLR4/MyD88/NF-κB (p65) pathway activation in vitro and in vivo. Dove 2020-10-23 /pmc/articles/PMC7591269/ /pubmed/33122931 http://dx.doi.org/10.2147/DMSO.S273952 Text en © 2020 Xu et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Xu, Yanmei
Xiong, Yan
Xu, Chen
Xu, Chuanwen
Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_full Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_fullStr Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_full_unstemmed Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_short Standard Puerarin Prevents Diabetic Renal Damage by Inhibiting miRNA-140-5p Expression
title_sort standard puerarin prevents diabetic renal damage by inhibiting mirna-140-5p expression
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591269/
https://www.ncbi.nlm.nih.gov/pubmed/33122931
http://dx.doi.org/10.2147/DMSO.S273952
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