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Verapamil Inhibits Mitochondria-Induced Reactive Oxygen Species and Dependent Apoptosis Pathways in Cerebral Transient Global Ischemia/Reperfusion

The prefrontal cortex is the largest lobe of the brain and is consequently involved in stroke. There is no comprehensive practical pharmacological strategy for ameliorating prefrontal cortex injury induced by cerebral ischemia. Therefore, we studied the neuroprotective properties of verapamil (Ver)...

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Autores principales: Jangholi, Ehsan, Sharifi, Zahra Nadia, Hoseinian, Mohammad, Zarrindast, Mohammad-Reza, Rahimi, Hamid Reza, Mowla, Ashkan, Aryan, Hoda, Javidi, Mohammad Amin, Parsa, Yekta, Ghaffarpasand, Fariborz, Yadollah-Damavandi, Soheila, Arani, Hamid Zaferani, Shahi, Farshad, Movassaghi, Shabnam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591985/
https://www.ncbi.nlm.nih.gov/pubmed/33133347
http://dx.doi.org/10.1155/2020/5872645
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author Jangholi, Ehsan
Sharifi, Zahra Nadia
Hoseinian, Mohammad
Zarrindast, Mohammad-Reza
Rahimi, Hamid Reza
Mowla, Ashkan
Aryan, Hoda
Javidi, Mohammad Amin
Parsa, Yekta
Ghaffarpasand, Fariborz
Yadollah-Damavandi, Soheila
Arani, Hamid Zaferani
Shahi, Farshad
Movassaghi, Shabnam
author_facet Jangholi, Ehsan
Sharifi, Zahra Nadia
Hoseinian, Mohammad
Zarrindast, Mohammad-Reza
Rahimi, Hamid Reza
Mowla, Ashkan
Aryan, Hoda
Javidi, Mohammad Amin
Parsa, Yekta
Ghaffarpasand, Fariborz
Yadollah-Damavandi, Soheila
Arani, Hamid Zaferani
Shahi, Farshad
Movassaghi, Shabnam
author_sort Jangholi, Ehsan
collection PubMed
description The prefrontal cortex is the largest lobe of the brain and is consequently involved in stroke. There is no comprehensive practical pharmacological strategy for ameliorating prefrontal cortex injury induced by cerebral ischemia. Therefore, we studied the neuroprotective properties of verapamil (Ver) on mitochondrial dysfunction and morphological features of apoptosis in transient global ischemia/reperfusion (I/R). Ninety-six Wistar rats were allocated into four groups: control, I/R, I/R+Ver (10 mg/kg twice 1 hour prior to ischemia and 1 hour after reperfusion phase), and I/R+NaCl (vehicle). Animals were sacrificed, and mitochondrial dysfunction parameters (i.e., mitochondrial swelling, mitochondrial membrane potential, ATP concentration, ROS production, and cytochrome c release), antioxidant defense (i.e., superoxide dismutase, malondialdehyde, glutathione peroxidase, catalase, and caspase-3 activation), and morphological features of apoptosis were determined. The results showed that mitochondrial damage, impairment of antioxidant defense system, and apoptosis were significantly more prevalent in the I/R group in comparison with the other groups. Ver decreased mitochondrial damage by reducing oxidative stress, augmented the activity of antioxidant enzymes in the brain, and decreased apoptosis in the I/R neurons. The current study confirmed the role of oxidative stress and mitochondrial dysfunction in I/R progression and indicated the possible antioxidative mechanism of the neuroprotective activities of Ver.
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spelling pubmed-75919852020-10-30 Verapamil Inhibits Mitochondria-Induced Reactive Oxygen Species and Dependent Apoptosis Pathways in Cerebral Transient Global Ischemia/Reperfusion Jangholi, Ehsan Sharifi, Zahra Nadia Hoseinian, Mohammad Zarrindast, Mohammad-Reza Rahimi, Hamid Reza Mowla, Ashkan Aryan, Hoda Javidi, Mohammad Amin Parsa, Yekta Ghaffarpasand, Fariborz Yadollah-Damavandi, Soheila Arani, Hamid Zaferani Shahi, Farshad Movassaghi, Shabnam Oxid Med Cell Longev Research Article The prefrontal cortex is the largest lobe of the brain and is consequently involved in stroke. There is no comprehensive practical pharmacological strategy for ameliorating prefrontal cortex injury induced by cerebral ischemia. Therefore, we studied the neuroprotective properties of verapamil (Ver) on mitochondrial dysfunction and morphological features of apoptosis in transient global ischemia/reperfusion (I/R). Ninety-six Wistar rats were allocated into four groups: control, I/R, I/R+Ver (10 mg/kg twice 1 hour prior to ischemia and 1 hour after reperfusion phase), and I/R+NaCl (vehicle). Animals were sacrificed, and mitochondrial dysfunction parameters (i.e., mitochondrial swelling, mitochondrial membrane potential, ATP concentration, ROS production, and cytochrome c release), antioxidant defense (i.e., superoxide dismutase, malondialdehyde, glutathione peroxidase, catalase, and caspase-3 activation), and morphological features of apoptosis were determined. The results showed that mitochondrial damage, impairment of antioxidant defense system, and apoptosis were significantly more prevalent in the I/R group in comparison with the other groups. Ver decreased mitochondrial damage by reducing oxidative stress, augmented the activity of antioxidant enzymes in the brain, and decreased apoptosis in the I/R neurons. The current study confirmed the role of oxidative stress and mitochondrial dysfunction in I/R progression and indicated the possible antioxidative mechanism of the neuroprotective activities of Ver. Hindawi 2020-10-17 /pmc/articles/PMC7591985/ /pubmed/33133347 http://dx.doi.org/10.1155/2020/5872645 Text en Copyright © 2020 Ehsan Jangholi et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jangholi, Ehsan
Sharifi, Zahra Nadia
Hoseinian, Mohammad
Zarrindast, Mohammad-Reza
Rahimi, Hamid Reza
Mowla, Ashkan
Aryan, Hoda
Javidi, Mohammad Amin
Parsa, Yekta
Ghaffarpasand, Fariborz
Yadollah-Damavandi, Soheila
Arani, Hamid Zaferani
Shahi, Farshad
Movassaghi, Shabnam
Verapamil Inhibits Mitochondria-Induced Reactive Oxygen Species and Dependent Apoptosis Pathways in Cerebral Transient Global Ischemia/Reperfusion
title Verapamil Inhibits Mitochondria-Induced Reactive Oxygen Species and Dependent Apoptosis Pathways in Cerebral Transient Global Ischemia/Reperfusion
title_full Verapamil Inhibits Mitochondria-Induced Reactive Oxygen Species and Dependent Apoptosis Pathways in Cerebral Transient Global Ischemia/Reperfusion
title_fullStr Verapamil Inhibits Mitochondria-Induced Reactive Oxygen Species and Dependent Apoptosis Pathways in Cerebral Transient Global Ischemia/Reperfusion
title_full_unstemmed Verapamil Inhibits Mitochondria-Induced Reactive Oxygen Species and Dependent Apoptosis Pathways in Cerebral Transient Global Ischemia/Reperfusion
title_short Verapamil Inhibits Mitochondria-Induced Reactive Oxygen Species and Dependent Apoptosis Pathways in Cerebral Transient Global Ischemia/Reperfusion
title_sort verapamil inhibits mitochondria-induced reactive oxygen species and dependent apoptosis pathways in cerebral transient global ischemia/reperfusion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591985/
https://www.ncbi.nlm.nih.gov/pubmed/33133347
http://dx.doi.org/10.1155/2020/5872645
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