The pseudogene problem and RT-qPCR data normalization; SYMPK: a suitable reference gene for papillary thyroid carcinoma

In RT-qPCR, accuracy requires multiple levels of standardization, but results could be obfuscated by human errors and technical limitations. Data normalization against suitable reference genes is critical, yet their observed expression can be confounded by pseudogenes. Eight reference genes were sel...

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Detalles Bibliográficos
Autores principales: Javadirad, Seyed-Morteza, Mokhtari, Mohammad, Esfandiarpour, Ghazal, Kolahdouzan, Mohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592052/
https://www.ncbi.nlm.nih.gov/pubmed/33110161
http://dx.doi.org/10.1038/s41598-020-75495-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592052/
https://www.ncbi.nlm.nih.gov/pubmed/33110161
http://dx.doi.org/10.1038/s41598-020-75495-7

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