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The pseudogene problem and RT-qPCR data normalization; SYMPK: a suitable reference gene for papillary thyroid carcinoma
In RT-qPCR, accuracy requires multiple levels of standardization, but results could be obfuscated by human errors and technical limitations. Data normalization against suitable reference genes is critical, yet their observed expression can be confounded by pseudogenes. Eight reference genes were sel...
Autores principales: | Javadirad, Seyed-Morteza, Mokhtari, Mohammad, Esfandiarpour, Ghazal, Kolahdouzan, Mohsen |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592052/ https://www.ncbi.nlm.nih.gov/pubmed/33110161 http://dx.doi.org/10.1038/s41598-020-75495-7 |
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