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Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3

BACKGROUND: Lung cancer (LC) is a malignant tumor originating in the bronchial mucosa or gland of the lung. Circular RNAs (circRNAs) are proved to be key regulators of tumor progression. However, the regulatory effect of circ_0001421 on lung cancer tumorigenesis remains unclear. METHODS: The express...

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Autores principales: Zhang, Koudong, Hu, Hang, Xu, Juan, Qiu, Limin, Chen, Haitao, Jiang, Xingzhi, Jiang, Yongqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592370/
https://www.ncbi.nlm.nih.gov/pubmed/33109222
http://dx.doi.org/10.1186/s13000-020-01048-1
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author Zhang, Koudong
Hu, Hang
Xu, Juan
Qiu, Limin
Chen, Haitao
Jiang, Xingzhi
Jiang, Yongqian
author_facet Zhang, Koudong
Hu, Hang
Xu, Juan
Qiu, Limin
Chen, Haitao
Jiang, Xingzhi
Jiang, Yongqian
author_sort Zhang, Koudong
collection PubMed
description BACKGROUND: Lung cancer (LC) is a malignant tumor originating in the bronchial mucosa or gland of the lung. Circular RNAs (circRNAs) are proved to be key regulators of tumor progression. However, the regulatory effect of circ_0001421 on lung cancer tumorigenesis remains unclear. METHODS: The expression levels of circ_0001421, microRNA-4677-3p (miR-4677-3p) and cell division cycle associated 3 (CDCA3) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Methyl thiazolyl tetrazolium (MTT), Transwell and Tumor formation assays were performed to explore the role of circ_0001421 in LC. Glucose consumption and lactate production were examined by a Glucose assay kit and a Lactic Acid assay kit. Western blot was utilized to examine the protein levels of Hexokinase 2 (HK2) and CDCA3. The interaction between miR-4677-3p and circ_0001421 or CDCA3 was confirmed by dual-luciferase reporter assay. RESULTS: Circ_0001421 was increased in LC tissues and cells, and knockdown of circ_0001421 repressed cell proliferation, migration, invasion and glycolysis in vitro. Meanwhile, circ_0001421 knockdown inhibited LC tumor growth in vivo. Mechanistically, circ_0001421 could bind to miR-4677-3p, and CDCA3 was a target of miR-4677-3p. Rescue assays manifested that silencing miR-4677-3p or CDCA3 overexpression reversed circ_0001421 knockdown-mediated suppression on cell proliferation, migration, invasion and glycolysis in LC cells. CONCLUSION: Circ_0001421 promoted cell proliferation, migration, invasion and glycolysis in LC by regulating the miR-4677-3p/CDCA3 axis, which providing a new mechanism for LC tumor progression.
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spelling pubmed-75923702020-10-29 Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3 Zhang, Koudong Hu, Hang Xu, Juan Qiu, Limin Chen, Haitao Jiang, Xingzhi Jiang, Yongqian Diagn Pathol Research BACKGROUND: Lung cancer (LC) is a malignant tumor originating in the bronchial mucosa or gland of the lung. Circular RNAs (circRNAs) are proved to be key regulators of tumor progression. However, the regulatory effect of circ_0001421 on lung cancer tumorigenesis remains unclear. METHODS: The expression levels of circ_0001421, microRNA-4677-3p (miR-4677-3p) and cell division cycle associated 3 (CDCA3) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Methyl thiazolyl tetrazolium (MTT), Transwell and Tumor formation assays were performed to explore the role of circ_0001421 in LC. Glucose consumption and lactate production were examined by a Glucose assay kit and a Lactic Acid assay kit. Western blot was utilized to examine the protein levels of Hexokinase 2 (HK2) and CDCA3. The interaction between miR-4677-3p and circ_0001421 or CDCA3 was confirmed by dual-luciferase reporter assay. RESULTS: Circ_0001421 was increased in LC tissues and cells, and knockdown of circ_0001421 repressed cell proliferation, migration, invasion and glycolysis in vitro. Meanwhile, circ_0001421 knockdown inhibited LC tumor growth in vivo. Mechanistically, circ_0001421 could bind to miR-4677-3p, and CDCA3 was a target of miR-4677-3p. Rescue assays manifested that silencing miR-4677-3p or CDCA3 overexpression reversed circ_0001421 knockdown-mediated suppression on cell proliferation, migration, invasion and glycolysis in LC cells. CONCLUSION: Circ_0001421 promoted cell proliferation, migration, invasion and glycolysis in LC by regulating the miR-4677-3p/CDCA3 axis, which providing a new mechanism for LC tumor progression. BioMed Central 2020-10-28 /pmc/articles/PMC7592370/ /pubmed/33109222 http://dx.doi.org/10.1186/s13000-020-01048-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Koudong
Hu, Hang
Xu, Juan
Qiu, Limin
Chen, Haitao
Jiang, Xingzhi
Jiang, Yongqian
Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3
title Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3
title_full Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3
title_fullStr Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3
title_full_unstemmed Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3
title_short Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3
title_sort circ_0001421 facilitates glycolysis and lung cancer development by regulating mir-4677-3p/cdca3
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592370/
https://www.ncbi.nlm.nih.gov/pubmed/33109222
http://dx.doi.org/10.1186/s13000-020-01048-1
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