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A new perfusion culture method with a self-organized capillary network

A lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial...

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Autores principales: Sugihara, Kei, Yamaguchi, Yoshimi, Usui, Shiori, Nashimoto, Yuji, Hanada, Sanshiro, Kiyokawa, Etsuko, Uemura, Akiyoshi, Yokokawa, Ryuji, Nishiyama, Koichi, Miura, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592787/
https://www.ncbi.nlm.nih.gov/pubmed/33112918
http://dx.doi.org/10.1371/journal.pone.0240552
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author Sugihara, Kei
Yamaguchi, Yoshimi
Usui, Shiori
Nashimoto, Yuji
Hanada, Sanshiro
Kiyokawa, Etsuko
Uemura, Akiyoshi
Yokokawa, Ryuji
Nishiyama, Koichi
Miura, Takashi
author_facet Sugihara, Kei
Yamaguchi, Yoshimi
Usui, Shiori
Nashimoto, Yuji
Hanada, Sanshiro
Kiyokawa, Etsuko
Uemura, Akiyoshi
Yokokawa, Ryuji
Nishiyama, Koichi
Miura, Takashi
author_sort Sugihara, Kei
collection PubMed
description A lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial cells to generate a capillary network and a static pressure difference for culture medium circulation, which can be easily introduced to standard biological laboratories and enables long-term cultivation of vascular structures. Using this culture system, we perfused the lumen of the self-organized capillary network and observed a flow-induced vascular remodeling process, cell shape changes, and collective cell migration. We also observed an increase in cell proliferation around the self-organized vasculature induced by flow, indicating functional perfusion of the culture medium. We also reconstructed extravasation of tumor and inflammatory cells, and circulation inside spheroids including endothelial cells and human lung fibroblasts. In conclusion, this system is a promising tool to elucidate the mechanisms of various biological processes related to vascular flow.
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spelling pubmed-75927872020-11-02 A new perfusion culture method with a self-organized capillary network Sugihara, Kei Yamaguchi, Yoshimi Usui, Shiori Nashimoto, Yuji Hanada, Sanshiro Kiyokawa, Etsuko Uemura, Akiyoshi Yokokawa, Ryuji Nishiyama, Koichi Miura, Takashi PLoS One Research Article A lack of perfusion has been one of the most significant obstacles for three-dimensional culture systems of organoids and embryonic tissues. Here, we developed a simple and reliable method to implement a perfusable capillary network in vitro. The method employed the self-organization of endothelial cells to generate a capillary network and a static pressure difference for culture medium circulation, which can be easily introduced to standard biological laboratories and enables long-term cultivation of vascular structures. Using this culture system, we perfused the lumen of the self-organized capillary network and observed a flow-induced vascular remodeling process, cell shape changes, and collective cell migration. We also observed an increase in cell proliferation around the self-organized vasculature induced by flow, indicating functional perfusion of the culture medium. We also reconstructed extravasation of tumor and inflammatory cells, and circulation inside spheroids including endothelial cells and human lung fibroblasts. In conclusion, this system is a promising tool to elucidate the mechanisms of various biological processes related to vascular flow. Public Library of Science 2020-10-28 /pmc/articles/PMC7592787/ /pubmed/33112918 http://dx.doi.org/10.1371/journal.pone.0240552 Text en © 2020 Sugihara et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sugihara, Kei
Yamaguchi, Yoshimi
Usui, Shiori
Nashimoto, Yuji
Hanada, Sanshiro
Kiyokawa, Etsuko
Uemura, Akiyoshi
Yokokawa, Ryuji
Nishiyama, Koichi
Miura, Takashi
A new perfusion culture method with a self-organized capillary network
title A new perfusion culture method with a self-organized capillary network
title_full A new perfusion culture method with a self-organized capillary network
title_fullStr A new perfusion culture method with a self-organized capillary network
title_full_unstemmed A new perfusion culture method with a self-organized capillary network
title_short A new perfusion culture method with a self-organized capillary network
title_sort new perfusion culture method with a self-organized capillary network
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592787/
https://www.ncbi.nlm.nih.gov/pubmed/33112918
http://dx.doi.org/10.1371/journal.pone.0240552
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