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Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics
Multiple sclerosis (MS) is a chronic, immune-mediated demyelinating disease of the central nervous system of unclear etiology, but there is some evidence that viral infections could be responsible for triggering autoimmune mechanisms against myelin. We searched for viral RNA and DNA in cerebrospinal...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592794/ https://www.ncbi.nlm.nih.gov/pubmed/33112911 http://dx.doi.org/10.1371/journal.pone.0240601 |
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author | Perlejewski, Karol Bukowska-Ośko, Iwona Rydzanicz, Małgorzata Dzieciątkowski, Tomasz Zakrzewska-Pniewska, Beata Podlecka-Piętowska, Aleksandra Filipiak, Agata Barć, Krzysztof Caraballo Cortés, Kamila Pawełczyk, Agnieszka Radkowski, Marek Laskus, Tomasz |
author_facet | Perlejewski, Karol Bukowska-Ośko, Iwona Rydzanicz, Małgorzata Dzieciątkowski, Tomasz Zakrzewska-Pniewska, Beata Podlecka-Piętowska, Aleksandra Filipiak, Agata Barć, Krzysztof Caraballo Cortés, Kamila Pawełczyk, Agnieszka Radkowski, Marek Laskus, Tomasz |
author_sort | Perlejewski, Karol |
collection | PubMed |
description | Multiple sclerosis (MS) is a chronic, immune-mediated demyelinating disease of the central nervous system of unclear etiology, but there is some evidence that viral infections could be responsible for triggering autoimmune mechanisms against myelin. We searched for viral RNA and DNA in cerebrospinal fluid (CSF) of 34 MS patients and 13 controls using RT-PCR/PCR against common neurotropic viruses. In addition, shotgun DNA- and RNA-based metagenomics were done in 13 MS patients and 4 controls. Specific quantitative real-time RT-PCR/PCR testing revealed the presence of viral nucleic acid in seven (20.59%) MS patients and in one (7.69%) control patient. In MS patients the most frequently detected was human herpesvirus type 6 (HHV-6; 3 cases; 8.82%); followed by Epstein-Barr virus (EBV; 2 cases; 5.88%), varicella zoster virus (VZV; 1 case; 2.94%) and Enterovirus (EV; 1 case; 2.94%). The single identified virus among controls was EBV (7.69%). DNA and RNA metagenomic assays did not identify any known eukaryotic viruses even though three of the analyzed samples were low-level positive by specific quantitative real-time PCR. In conclusion, we detected the presence of Herpesviridae and occasionally Enteroviridae in CSF from patients with MS but their prevalence was not significantly higher than among controls. Metagenomic analysis seems to be less sensitive than real-time RT-PCR/PCR and it did not detect any potential viral pathogens. |
format | Online Article Text |
id | pubmed-7592794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-75927942020-11-02 Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics Perlejewski, Karol Bukowska-Ośko, Iwona Rydzanicz, Małgorzata Dzieciątkowski, Tomasz Zakrzewska-Pniewska, Beata Podlecka-Piętowska, Aleksandra Filipiak, Agata Barć, Krzysztof Caraballo Cortés, Kamila Pawełczyk, Agnieszka Radkowski, Marek Laskus, Tomasz PLoS One Research Article Multiple sclerosis (MS) is a chronic, immune-mediated demyelinating disease of the central nervous system of unclear etiology, but there is some evidence that viral infections could be responsible for triggering autoimmune mechanisms against myelin. We searched for viral RNA and DNA in cerebrospinal fluid (CSF) of 34 MS patients and 13 controls using RT-PCR/PCR against common neurotropic viruses. In addition, shotgun DNA- and RNA-based metagenomics were done in 13 MS patients and 4 controls. Specific quantitative real-time RT-PCR/PCR testing revealed the presence of viral nucleic acid in seven (20.59%) MS patients and in one (7.69%) control patient. In MS patients the most frequently detected was human herpesvirus type 6 (HHV-6; 3 cases; 8.82%); followed by Epstein-Barr virus (EBV; 2 cases; 5.88%), varicella zoster virus (VZV; 1 case; 2.94%) and Enterovirus (EV; 1 case; 2.94%). The single identified virus among controls was EBV (7.69%). DNA and RNA metagenomic assays did not identify any known eukaryotic viruses even though three of the analyzed samples were low-level positive by specific quantitative real-time PCR. In conclusion, we detected the presence of Herpesviridae and occasionally Enteroviridae in CSF from patients with MS but their prevalence was not significantly higher than among controls. Metagenomic analysis seems to be less sensitive than real-time RT-PCR/PCR and it did not detect any potential viral pathogens. Public Library of Science 2020-10-28 /pmc/articles/PMC7592794/ /pubmed/33112911 http://dx.doi.org/10.1371/journal.pone.0240601 Text en © 2020 Perlejewski et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Perlejewski, Karol Bukowska-Ośko, Iwona Rydzanicz, Małgorzata Dzieciątkowski, Tomasz Zakrzewska-Pniewska, Beata Podlecka-Piętowska, Aleksandra Filipiak, Agata Barć, Krzysztof Caraballo Cortés, Kamila Pawełczyk, Agnieszka Radkowski, Marek Laskus, Tomasz Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics |
title | Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics |
title_full | Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics |
title_fullStr | Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics |
title_full_unstemmed | Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics |
title_short | Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics |
title_sort | search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time pcr and metagenomics |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7592794/ https://www.ncbi.nlm.nih.gov/pubmed/33112911 http://dx.doi.org/10.1371/journal.pone.0240601 |
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