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The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats

BACKGROUND: Incisional hernia is a frequent complication of abdominal wall incision and has a high rate of recurrence. Most of the studies stated that non-absorbable sutures decreased incisional hernia incidences, but some stated otherwise. We aimed to compare the collagen type I/III ratio between m...

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Autores principales: Sofii, Imam, Dipoyono, Wisnu, Prima, Heryu, Sari, Yessy Martha, Fauzi, Aditya Rifqi, Gunadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593262/
https://www.ncbi.nlm.nih.gov/pubmed/33145017
http://dx.doi.org/10.1016/j.amsu.2020.10.033
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author Sofii, Imam
Dipoyono, Wisnu
Prima, Heryu
Sari, Yessy Martha
Fauzi, Aditya Rifqi
Gunadi
author_facet Sofii, Imam
Dipoyono, Wisnu
Prima, Heryu
Sari, Yessy Martha
Fauzi, Aditya Rifqi
Gunadi
author_sort Sofii, Imam
collection PubMed
description BACKGROUND: Incisional hernia is a frequent complication of abdominal wall incision and has a high rate of recurrence. Most of the studies stated that non-absorbable sutures decreased incisional hernia incidences, but some stated otherwise. We aimed to compare the collagen type I/III ratio between monofilament non-absorbable sutures and multifilament absorbable sutures for abdominal fascia closure in Wistar albino rats. METHODS: Forty rats were divided into four groups. Groups 1 and 3 were sutured with monofilament non-absorbable (polyvinylidene fluoride). Groups 2 and 4 were sutured with multifilament absorbable (polyglycolide). Then, groups 1 and 2 were euthanized on day 4 (POD 4), while groups 3 and 4 were euthanized on day 7 (POD 7). Samples of fascia (1 × 0.5 cm) were taken for analysis. Collagen I/III ratios were measured using immunohistochemistry staining methods. RESULTS: While the expression of collagen I was not significantly different between monofilament non-absorbable and multifilament absorbable at POD 4 and 7 (p = 0.45 and 0.81, respectively), the expression of collagen III reached a significant level with p-values of 0.0003 and 0.0004 for POD 4 and 7, respectively. Moreover, the collagen I/III ratio was also significantly different between the two groups either at POD 4 (0.88 ± 0.23 vs. 0.53 ± 0.08; p = 0.0003) and 7 (1.77 ± 0.65 vs. 1.03 ± 0.28; p = 0.004). CONCLUSIONS: Monofilament non-absorbable sutures show a significantly higher collagen I/III ratio than multifilament absorbable sutures for abdominal fascia closure in rats. Our findings imply that the usage of monofilament non-absorbable sutures might have a beneficial effect on decreasing the incisional hernia occurrence.
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spelling pubmed-75932622020-11-02 The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats Sofii, Imam Dipoyono, Wisnu Prima, Heryu Sari, Yessy Martha Fauzi, Aditya Rifqi Gunadi Ann Med Surg (Lond) Experimental Research BACKGROUND: Incisional hernia is a frequent complication of abdominal wall incision and has a high rate of recurrence. Most of the studies stated that non-absorbable sutures decreased incisional hernia incidences, but some stated otherwise. We aimed to compare the collagen type I/III ratio between monofilament non-absorbable sutures and multifilament absorbable sutures for abdominal fascia closure in Wistar albino rats. METHODS: Forty rats were divided into four groups. Groups 1 and 3 were sutured with monofilament non-absorbable (polyvinylidene fluoride). Groups 2 and 4 were sutured with multifilament absorbable (polyglycolide). Then, groups 1 and 2 were euthanized on day 4 (POD 4), while groups 3 and 4 were euthanized on day 7 (POD 7). Samples of fascia (1 × 0.5 cm) were taken for analysis. Collagen I/III ratios were measured using immunohistochemistry staining methods. RESULTS: While the expression of collagen I was not significantly different between monofilament non-absorbable and multifilament absorbable at POD 4 and 7 (p = 0.45 and 0.81, respectively), the expression of collagen III reached a significant level with p-values of 0.0003 and 0.0004 for POD 4 and 7, respectively. Moreover, the collagen I/III ratio was also significantly different between the two groups either at POD 4 (0.88 ± 0.23 vs. 0.53 ± 0.08; p = 0.0003) and 7 (1.77 ± 0.65 vs. 1.03 ± 0.28; p = 0.004). CONCLUSIONS: Monofilament non-absorbable sutures show a significantly higher collagen I/III ratio than multifilament absorbable sutures for abdominal fascia closure in rats. Our findings imply that the usage of monofilament non-absorbable sutures might have a beneficial effect on decreasing the incisional hernia occurrence. Elsevier 2020-10-23 /pmc/articles/PMC7593262/ /pubmed/33145017 http://dx.doi.org/10.1016/j.amsu.2020.10.033 Text en © 2020 Published by Elsevier Ltd on behalf of IJS Publishing Group Ltd. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Experimental Research
Sofii, Imam
Dipoyono, Wisnu
Prima, Heryu
Sari, Yessy Martha
Fauzi, Aditya Rifqi
Gunadi
The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats
title The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats
title_full The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats
title_fullStr The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats
title_full_unstemmed The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats
title_short The effect of different suturing materials for abdominal fascia wound closure on the collagen I/III expression ratio in rats
title_sort effect of different suturing materials for abdominal fascia wound closure on the collagen i/iii expression ratio in rats
topic Experimental Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593262/
https://www.ncbi.nlm.nih.gov/pubmed/33145017
http://dx.doi.org/10.1016/j.amsu.2020.10.033
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