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Network Pharmacology-Based Study on the Mechanism of Pinellia ternata in Asthma Treatment
BACKGROUND: Pinellia ternata (PT), a medicinal plant, has had an extensive application in the treatment of asthma in China, whereas its underlying pharmacological mechanisms remain unclear. METHODS: Firstly, a network pharmacology method was adopted to collect activated components of PT from Traditi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593714/ https://www.ncbi.nlm.nih.gov/pubmed/33133221 http://dx.doi.org/10.1155/2020/9732626 |
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author | Lyu, Yanmin Chen, Xiangjing Xia, Qing Zhang, Shanshan Yao, Chengfang |
author_facet | Lyu, Yanmin Chen, Xiangjing Xia, Qing Zhang, Shanshan Yao, Chengfang |
author_sort | Lyu, Yanmin |
collection | PubMed |
description | BACKGROUND: Pinellia ternata (PT), a medicinal plant, has had an extensive application in the treatment of asthma in China, whereas its underlying pharmacological mechanisms remain unclear. METHODS: Firstly, a network pharmacology method was adopted to collect activated components of PT from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Targets of PT were assessed by exploiting the PharmMapper website; asthma-related targets were collected from the OMIM website, and target-target interaction networks were built. Secondly, critical nodes exhibiting high possibility were identified as the hub nodes in the network, which were employed to conduct Gene Ontology (GO) comment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis. Finally, the tissue expression profiles of key candidate genes were identified by the Gene Expression Omnibus (GEO) database, and the therapeutic effect of PT was verified by an animal experiment. RESULTS: 57 achievable targets of PT on asthma were confirmed as hub nodes through using the network pharmacology method. As revealed from the KEGG enrichment analysis, the signaling pathways were notably enriched in pathways of the T-cell receptor signaling pathway, JAK-STAT signaling pathway, and cytokine-cytokine receptor interaction. The expression profiles of candidate genes including Mmp2, Nr3c1, il-10, il-4, il-13, il-17a, il-2, tlr4, tlr9, ccl2, csf2, and vefgα were identified. Moreover, according to transcriptome RNA sequencing data from lung tissues of allergic mice compared to normal mice, the mRNA level of Mmp2 and il-4 was upregulated (P < 0.001). In animal experiments, PT could alleviate the allergic response of mice by inhibiting the activation of T-helper type 2 (TH2) cells and the expression of Mmp2 and il-4. CONCLUSIONS: Our study provides candidate genes that may be either used for future studies related to diagnosis/prognosis or as targets for asthma management. Besides, animal experiments showed that PT could treat asthma by regulating the expression of Mmp2 and il-4. |
format | Online Article Text |
id | pubmed-7593714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-75937142020-10-30 Network Pharmacology-Based Study on the Mechanism of Pinellia ternata in Asthma Treatment Lyu, Yanmin Chen, Xiangjing Xia, Qing Zhang, Shanshan Yao, Chengfang Evid Based Complement Alternat Med Research Article BACKGROUND: Pinellia ternata (PT), a medicinal plant, has had an extensive application in the treatment of asthma in China, whereas its underlying pharmacological mechanisms remain unclear. METHODS: Firstly, a network pharmacology method was adopted to collect activated components of PT from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Targets of PT were assessed by exploiting the PharmMapper website; asthma-related targets were collected from the OMIM website, and target-target interaction networks were built. Secondly, critical nodes exhibiting high possibility were identified as the hub nodes in the network, which were employed to conduct Gene Ontology (GO) comment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis. Finally, the tissue expression profiles of key candidate genes were identified by the Gene Expression Omnibus (GEO) database, and the therapeutic effect of PT was verified by an animal experiment. RESULTS: 57 achievable targets of PT on asthma were confirmed as hub nodes through using the network pharmacology method. As revealed from the KEGG enrichment analysis, the signaling pathways were notably enriched in pathways of the T-cell receptor signaling pathway, JAK-STAT signaling pathway, and cytokine-cytokine receptor interaction. The expression profiles of candidate genes including Mmp2, Nr3c1, il-10, il-4, il-13, il-17a, il-2, tlr4, tlr9, ccl2, csf2, and vefgα were identified. Moreover, according to transcriptome RNA sequencing data from lung tissues of allergic mice compared to normal mice, the mRNA level of Mmp2 and il-4 was upregulated (P < 0.001). In animal experiments, PT could alleviate the allergic response of mice by inhibiting the activation of T-helper type 2 (TH2) cells and the expression of Mmp2 and il-4. CONCLUSIONS: Our study provides candidate genes that may be either used for future studies related to diagnosis/prognosis or as targets for asthma management. Besides, animal experiments showed that PT could treat asthma by regulating the expression of Mmp2 and il-4. Hindawi 2020-10-20 /pmc/articles/PMC7593714/ /pubmed/33133221 http://dx.doi.org/10.1155/2020/9732626 Text en Copyright © 2020 Yanmin Lyu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lyu, Yanmin Chen, Xiangjing Xia, Qing Zhang, Shanshan Yao, Chengfang Network Pharmacology-Based Study on the Mechanism of Pinellia ternata in Asthma Treatment |
title | Network Pharmacology-Based Study on the Mechanism of Pinellia ternata in Asthma Treatment |
title_full | Network Pharmacology-Based Study on the Mechanism of Pinellia ternata in Asthma Treatment |
title_fullStr | Network Pharmacology-Based Study on the Mechanism of Pinellia ternata in Asthma Treatment |
title_full_unstemmed | Network Pharmacology-Based Study on the Mechanism of Pinellia ternata in Asthma Treatment |
title_short | Network Pharmacology-Based Study on the Mechanism of Pinellia ternata in Asthma Treatment |
title_sort | network pharmacology-based study on the mechanism of pinellia ternata in asthma treatment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7593714/ https://www.ncbi.nlm.nih.gov/pubmed/33133221 http://dx.doi.org/10.1155/2020/9732626 |
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