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Laser Capture Microdissection-Based RNA Microsequencing Reveals Optic Nerve Crush-Related Early mRNA Alterations in Retinal Ganglion Cell Layer

PURPOSE: To establish a method of laser capture microdissection (LCM) and RNA microsequencing for exploring optic nerve crush (ONC)–related early mRNA alterations in retinal ganglion cell (RGC) layer. METHODS: An LCM protocol was developed using retinal tissue sections to obtain high-quality RNA for...

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Detalles Bibliográficos
Autores principales:	Pan, Dongyan, Xu, Mengqiao, Chang, Xin, Xia, Mao, Fang, Yibin, Fu, Yinghua, Shen, Wei, Wang, Yue, Sun, Xiaodong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Association for Research in Vision and Ophthalmology 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594581/ https://www.ncbi.nlm.nih.gov/pubmed/33173609 http://dx.doi.org/10.1167/tvst.9.11.30

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