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The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum

Paphiopedilum spicerianum (H. G. Reichenbach) Pfitzer is an endangered and threatened orchid species in China. The genetic and molecular data of this orchid species is deficient. With the aim to identify appropriate chloroplast markers for the use in a conservation biology study, the complete chloro...

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Autores principales: Ge, Li-Ping, Tang, Lu, Li, Lu, Luo, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594755/
https://www.ncbi.nlm.nih.gov/pubmed/33367030
http://dx.doi.org/10.1080/23802359.2020.1830727
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author Ge, Li-Ping
Tang, Lu
Li, Lu
Luo, Yan
author_facet Ge, Li-Ping
Tang, Lu
Li, Lu
Luo, Yan
author_sort Ge, Li-Ping
collection PubMed
description Paphiopedilum spicerianum (H. G. Reichenbach) Pfitzer is an endangered and threatened orchid species in China. The genetic and molecular data of this orchid species is deficient. With the aim to identify appropriate chloroplast markers for the use in a conservation biology study, the complete chloroplast genome of P. spicerianum was reported. We found that the chloroplast genome of P. spicerianum is 157,292 bp in length, containing a large single-copy region of 87,252 bp, a small single-copy region of 1828 bp, and a pair of inverted repeats of 34,106 bp. Genome annotation predicted 105 unique genes, including 71 protein-coding genes, 30 tRNAs, and four rRNAs. Fourteen genes contained one intron and two genes (clpP and ycf3) had two introns. The GC content of the P. spicerianum was 35.8%. Phylogenetic analysis indicated P. spicerianum was closely related to P. purpuratum.
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spelling pubmed-75947552020-12-22 The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum Ge, Li-Ping Tang, Lu Li, Lu Luo, Yan Mitochondrial DNA B Resour Mitogenome Announcement Paphiopedilum spicerianum (H. G. Reichenbach) Pfitzer is an endangered and threatened orchid species in China. The genetic and molecular data of this orchid species is deficient. With the aim to identify appropriate chloroplast markers for the use in a conservation biology study, the complete chloroplast genome of P. spicerianum was reported. We found that the chloroplast genome of P. spicerianum is 157,292 bp in length, containing a large single-copy region of 87,252 bp, a small single-copy region of 1828 bp, and a pair of inverted repeats of 34,106 bp. Genome annotation predicted 105 unique genes, including 71 protein-coding genes, 30 tRNAs, and four rRNAs. Fourteen genes contained one intron and two genes (clpP and ycf3) had two introns. The GC content of the P. spicerianum was 35.8%. Phylogenetic analysis indicated P. spicerianum was closely related to P. purpuratum. Taylor & Francis 2020-10-21 /pmc/articles/PMC7594755/ /pubmed/33367030 http://dx.doi.org/10.1080/23802359.2020.1830727 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mitogenome Announcement
Ge, Li-Ping
Tang, Lu
Li, Lu
Luo, Yan
The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum
title The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum
title_full The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum
title_fullStr The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum
title_full_unstemmed The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum
title_short The complete chloroplast genome of an endangered orchid Paphiopedilum spicerianum
title_sort complete chloroplast genome of an endangered orchid paphiopedilum spicerianum
topic Mitogenome Announcement
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594755/
https://www.ncbi.nlm.nih.gov/pubmed/33367030
http://dx.doi.org/10.1080/23802359.2020.1830727
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